Search results for "Cytoplasm"

453 results for "Cytoplasm" - showing 450-453

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IF (Immunofluorescence) (Immunofluorescence analysis of paraformaldehyde fixed CaCo-2 cells immobilized on Shi-fix™ coverslip, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).)

IF (Immunofluorescence) (Immunofluorescence analysis of paraformaldehyde fixed U937 cells immobilized on Shi-fix coverslip, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).)

IF (Immunofluorescence) (AAA13659 Immunofluorescence analysis of paraformaldehyde fixed Caco-2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).)

FCM (Flow Cytometry) (Flow cytometric analysis of paraformaldehyde fixed K562 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.)

IHC (Immunohistochemistry) (Negative Control showing staining of paraffin embedded Human Lung, with no primary antibody.)

IHC (Immunohistochemistry) ((6µg/ml) staining of paraffin embedded Human Lung. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.)

WB (Western Blot) ((0.5ug/ml) staining of Human Frontal Cortex lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

AIF1/IBA1, Polyclonal Antibody (Cat# AAA13659)

Full Name

Goat anti-AIF1/IBA1 (isoform 3) Antibody

Gene Names

AIF1; IBA1; IRT1; AIF-1; IRT-1

Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Dog

Applications

Immunohistochemistry, Immunofluorescence, Flow Cytometry

Purity

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

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IHC (Immunohistochemistry) (AAA30943 at 1/100 staining rat testicular tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30943 at 1/100 staining rat ovarian tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30943 at 1/100 staining mouse testis tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30943 at 1/100 staining mouse spleen tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistchemistry) (AAA30943 at 1/100 staining mouse kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30943 at 1/100 staining human liver tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30943 at 1/100 staining human appendiceal tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistchemistry) (AAA30943 at 1/100 staining human skin tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA30943 at 1/100 staining human glioma tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

WB (Western Blot) (Western blot analysis of I kappaB epsilon phosphorylation expression in TNF-a treated Jurkat whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

WB (Western Blot) (Western blot analysis of Phospho-I kappaB epsilon (Ser22) expression in various lysates)

IF (Immunofluorescence) (AAA30943 staining HeLa cells by ICC/IF. Cells were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 minutes at 37 degree C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37 degree C. A Alexa Fluor 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.)

I kappaB epsilon, Polyclonal Antibody (Cat# AAA30943)

Full Name

Phospho-I kappaB epsilon (Ser22) Antibody

Gene Names

NFKBIE; IKBE

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

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IF (Immunofluorescence) (Immunofluorescent staining of paraformaldehyde-fixed Ramos cells using CD22-Monospecific Mouse Monoclonal Antibody (BLCAM/1795) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Reddot.)

ELISA (Analysis of Protein Array containing more than 19,000 full-length human proteins using CD22-Monospecific Mouse Monoclonal Antibody (BLCAM/1795) Z-and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descendingorder of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)

FCM (Flow Cytometry) (Flow Cytometric Analysis of paraformaldehyde-fixed MOLT4 cells. CD22-Monospecific Mouse Monoclonal Antibody (BLCAM/1795) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).)

SDS-PAGE (SDS-PAGE Analysis Purified CD22-Monospecific Mouse Monoclonal Antibody (BLCAM/1795). Confirmation of Purity and Integrity of Antibody)

WB (Western Blot) (Western Blot Analysis of Raji cell lysate using CD22-Monospecific Mouse Monoclonal Antibody (BLCAM/1795).)

WB (Western Blot) (Western Blot Analysis of Raji and Ramos Cell Lysates using CD22 Mouse Monoclonal Antibody (BLCAM/1795).)

IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Tonsil stained with CD22 Mouse Monoclonal Antibody (BLCAM/1795).)

IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Spleen stained with CD22 Mouse Monoclonal Antibody (BLCAM/1795).)

CD22/BL-CAM, Monoclonal Antibody (Cat# AAA23895)

Full Name

CD22/BL-CAM (B-Cell Marker)

Gene Names

CD22; SIGLEC2; SIGLEC-2

Reactivity

Human. Others not known.

Applications

Western Blot, Immunohistochemistry

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453 results for "Cytoplasm" - showing 450-453

AIF1/IBA1, Polyclonal Antibody (Cat# AAA13659)

I kappaB epsilon, Polyclonal Antibody (Cat# AAA30943)

CD22/BL-CAM, Monoclonal Antibody (Cat# AAA23895)

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453 results for "Cytoplasm" - showing 450-453

AIF1/IBA1, Polyclonal Antibody (Cat# AAA13659)

I kappaB epsilon, Polyclonal Antibody (Cat# AAA30943)

CD22/BL-CAM, Monoclonal Antibody (Cat# AAA23895)

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