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Type
Reactivity
Gene Name
Isotype
Host
Application
Clone
115 results for " T cell activation" - showing 1-50
Application Data
(At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
Histone H3, Polyclonal Antibody (Cat# AAA31345)
Full Name
Acetyl-Histone H3 (Lys14) Antibody
Gene Names
HIST1H3A; H3/A; H3FA
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Bovine (100%)
Predicted Reactivity: Bovine (100%)
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Pricing
WB (Western Blot)
(HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAPRE2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPRE2.)
MAPRE2 / EB2, Monoclonal Antibody (Cat# AAA12371)
Full Name
Mouse Monoclonal [clone 1F3] (IgG2a) to Human MAPRE2 / EB2
Gene Names
MAPRE2; EB1; EB2; RP1
Reactivity
Human
Applications
Immunohistochemistry, Immunofluorescence, Western Blot, Flow Cytometry
Purity
Protein A/G Purified
Pricing
Application Data
(At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)
Bub1, Polyclonal Antibody (Cat# AAA31312)
Full Name
Phospho-Bub1 (Ser459) Antibody
Gene Names
BUB1; BUB1A; BUB1L; hBUB1
Reactivity
Human, Rat
Applications
Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistochemistry)
(At 1/100 staining Human colorectal cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Histone H3, Polyclonal Antibody (Cat# AAA31356)
Full Name
Acetyl-Histone H3 (Lys9/14/18/23/27) Antibody
Gene Names
HIST1H3A; H3/A; H3FA
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Bovine (100%)
Predicted Reactivity: Bovine (100%)
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Pricing
IHC (Immunohistochemistry)
(At 1/100 staining Human esophageal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
p53, Polyclonal Antibody (Cat# AAA31354)
Full Name
Acetyl-p53 (Lys373) Antibody
Gene Names
TP53; P53; BCC7; LFS1; TRP53
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (91%), Sheep (80%), Rabbit (91%), Dog (91%)
Predicted Reactivity: Pig (91%), Sheep (80%), Rabbit (91%), Dog (91%)
Applications
Western Blot, Immunohistochemistry, Peptide ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Pricing
Application Data
(Published customer image: Increased accumulation of repair-associated macrophages surrounding collaterals in ischemic hind limbs is PAR2-dependent. (A) Stainings of CD206-positive macrophages (green) and SMA-positive vessels (red) in non-ischemic (control) and ischemic (ligated) hind limbs of WT, PAR1-/- and PAR2-/- mice are shown. Nuclei were visualized with DAPI (blue). Arrows indicate single macrophages in the non-ischemic adductor. Quantification of the average number of repair-associated macrophages per vessel is indicated on the right. (B) Correlation between the number of CD206-positive macrophages in the ischemic tissues and the expression of CD11b and (C) CD115 on monocytes. ** p)
CD206, Monoclonal Antibody (Cat# AAA12117)
Full Name
RAT ANTI MOUSE CD206:Biotin
Gene Names
Mrc1; MR; CD206; AW259686
Applications
Flow Cytometry
Pricing
IHC (Immunohistochemistry)
(At 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
IRF7, Polyclonal Antibody (Cat# AAA31458)
Full Name
Phospho-IRF7 (Ser477) Antibody
Gene Names
IRF7; IRF7A; IRF7B; IRF7C; IRF7H; IRF-7H
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%)
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%)
Applications
Western Blot, Immunohistochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
Application Data
(Published customer image: Representative images of the inflammatory changes in the facial nucleus during axonal regeneration, one week following facial nerve transaction. a, b: CD11b immunoreactivity for microglia is increased in the axotomized facial nucleus, and microglia enwrap the facial motor neurons, e.g. at arrows. The regenerating neurons were retrogradely labelled with fluorogold. c, d: CD6- positive T-cells accumulated in the injured motor nucleus (arrows). They had little cytoplasm but dense nuclei (c) and were sometimes clustered around neurons retrogradely labelled with fluorogold (d). The scale bar in (a) also applies to (b) and that in (c) also applies to (d).From: Shokouhi et al. BMC Neuroscience 2010 11:13.)
CD11b, Monoclonal Antibody (Cat# AAA11853)
Full Name
MOUSE ANTI RAT CD11b:Biotin
Gene Names
ITGAM; CD11B
Applications
Immunohistochemistry, Flow Cytometry
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of THP-1 cells with CD40L antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
CD40L, Monoclonal Antibody (Cat# AAA30328)
Full Name
CD40L Antibody
Gene Names
CD40LG; IGM; IMD3; TRAP; gp39; CD154; CD40L; HIGM1; T-BAM; TNFSF5; hCD40L
Reactivity
Human, Mouse
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry
Purity
ProA affinity purified
Pricing
Application Data
(Published customer image: Increased accumulation of repair-associated macrophages surrounding collaterals in ischemic hind limbs is PAR2-dependent. (A) Stainings of CD206-positive macrophages (green) and SMA-positive vessels (red) in non-ischemic (control) and ischemic (ligated) hind limbs of WT, PAR1-/- and PAR2-/- mice are shown. Nuclei were visualized with DAPI (blue). Arrows indicate single macrophages in the non-ischemic adductor. Quantification of the average number of repair-associated macrophages per vessel is indicated on the right. (B) Correlation between the number of CD206-positive macrophages in the ischemic tissues and the expression of CD11b and (C) CD115 on monocytes. ** p)
CD206, Monoclonal Antibody (Cat# AAA12122)
Full Name
RAT ANTI MOUSE CD206:FITC
Gene Names
Mrc1; MR; CD206; AW259686
Applications
Flow Cytometry
Pricing
IHC (Immunohistochemistry)
(At 1/100 staining Human colorectal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
NFAT2, Polyclonal Antibody (Cat# AAA31381)
Full Name
Phospho-NFAT2 (Ser294) Antibody
Gene Names
NFATC1; NFAT2; NFATc; NF-ATC
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Bovine (85%), Rabbit (100%)
Predicted Reactivity: Bovine (85%), Rabbit (100%)
Applications
Western Blot, Immunohistochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
Application Data
(Published customer image: Increased accumulation of repair-associated macrophages surrounding collaterals in ischemic hind limbs is PAR2-dependent. (A) Stainings of CD206-positive macrophages (green) and SMA-positive vessels (red) in non-ischemic (control) and ischemic (ligated) hind limbs of WT, PAR1-/- and PAR2-/- mice are shown. Nuclei were visualized with DAPI (blue). Arrows indicate single macrophages in the non-ischemic adductor. Quantification of the average number of repair-associated macrophages per vessel is indicated on the right. (B) Correlation between the number of CD206-positive macrophages in the ischemic tissues and the expression of CD11b and (C) CD115 on monocytes. ** p)
CD206, Monoclonal Antibody (Cat# AAA12125)
Full Name
RAT ANTI MOUSE CD206:RPE
Gene Names
Mrc1; MR; CD206; AW259686
Applications
Flow Cytometry
Pricing
IHC (Immunohistochemistry)
(At 1/100 staining Human esophageal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
p53, Polyclonal Antibody (Cat# AAA31377)
Full Name
p53 Antibody
Gene Names
TP53; P53; BCC7; LFS1; TRP53
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (91%), Sheep (80%), Rabbit (91%), Dog (91%)
Predicted Reactivity: Pig (91%), Sheep (80%), Rabbit (91%), Dog (91%)
Applications
Western Blot, Immunohistochemistry, Peptide ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Pricing
Application Data
(Published customer image: Increased accumulation of repair-associated macrophages surrounding collaterals in ischemic hind limbs is PAR2-dependent. (A) Stainings of CD206-positive macrophages (green) and SMA-positive vessels (red) in non-ischemic (control) and ischemic (ligated) hind limbs of WT, PAR1-/- and PAR2-/- mice are shown. Nuclei were visualized with DAPI (blue). Arrows indicate single macrophages in the non-ischemic adductor. Quantification of the average number of repair-associated macrophages per vessel is indicated on the right. (B) Correlation between the number of CD206-positive macrophages in the ischemic tissues and the expression of CD11b and (C) CD115 on monocytes. ** p)
CD206, Monoclonal Antibody (Cat# AAA12120)
Full Name
RAT ANTI MOUSE CD206:FITC
Gene Names
Mrc1; MR; CD206; AW259686
Applications
Flow Cytometry
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of Jurkat cells with CD137 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).)
CD137, Monoclonal Antibody (Cat# AAA29865)
Full Name
CD137 Antibody
Gene Names
TNFRSF9; ILA; 4-1BB; CD137; CDw137
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Flow Cytometry
Purity
ProA affinity purified
Pricing
Application Data
(Published customer image Infiltration of GFP+ BM-cells in infarct and peri-infarct regions. (A-B) Dot plots of viable macrophages/granulocytes (CD11b+CD45high, top right quadrants) and microglia (CD11b+CD45dim, bottom right quadrants) in cortex from BM-chimeric unmanipulated mice and mice exposed to pMCAO. (C) Bar graph showing mean numbers of CD11b+CD45dim microglia and CD11b+CD45high macrophages/granulocytes in BM-chimeric mice 24 hours after pMCAO, subdivided based on expression of GFP (n = 5). Approximately 92% of of the CD45high population were GFP+. (D) Estimation and comparison of mean numbers of CD11b+CD45dim microglia in non-chimeric (n = 10) versus BM-chimeric mice (n = 5) 24 hours after of pMCAO shows significantly fewer CD11b+CD45dim microglial cells in irradiated mice. (E) Overview, showing distribution of infiltrating GFP+ BM-derived cells into infarct (IF) and peri-infarct (P-IF) regions 24 hours after pMCAO. (E-G) By 24 hours, GFP+ single cells (F) and vessel-associated aggregates of GFP+ cells (arrows in G) were observed in infarct and peri-infarct regions. Some of the vessel-associated cells were round, leukocyte-like cells (arrows) while others were elongated cells lining the vasculature (arrow heads in G and in insert). (H) Bar graph showing mean numbers of single GFP+ cells and vessel-associated aggregates of GFP+ cells in ipsi- and contralateral cortex 24 hours after surgery (n = 10). (I-P) Immunohistochemical staining of CD45.1 (I, K), CD45.2 (J, L), IgG2a (M, O) and CD45 (N, P) in ischemic tissue in BM-chimeric (I, J, M, N) and non-chimeric mice (K, L, O, P) 24 hours after pMCAO. N.D, none detected. Scale bars: 200 um (A), 10 um (B, C). 50 um (I-P) *P < 0.05, **P < 0.01, and ***P < 0.001.From: Clausen BH, Lambertsen KL, Babcock AA, Holm TH, Dagnaes-Hansen F, Finsen B. Interleukin-1beta and tumor necrosis factor-alpha are expressed by different subsets of microglia and macrophages after ischemic stroke in mice. J Neuroinflammation. 2008 Oct 23;5:46.)
CD11b, Monoclonal Antibody (Cat# AAA12182)
Full Name
RAT ANTI MOUSE CD11b:FITC
Gene Names
Itgam; CR3; CR3A; MAC1; Cd11b; Ly-40; Mac-1; Mac-1a; CD11b/CD18; F730045J24Rik
Applications
Flow Cytometry
Pricing
Application Data
(Published customer image: Representative images of the inflammatory changes in the facial nucleus during axonal regeneration, one week following facial nerve transaction. a, b: CD11b immunoreactivity for microglia is increased in the axotomized facial nucleus, and microglia enwrap the facial motor neurons, e.g. at arrows. The regenerating neurons were retrogradely labelled with fluorogold. c, d: CD6- positive T-cells accumulated in the injured motor nucleus (arrows). They had little cytoplasm but dense nuclei (c) and were sometimes clustered around neurons retrogradely labelled with fluorogold (d). The scale bar in (a) also applies to (b) and that in (c) also applies to (d).From: Shokouhi et al. BMC Neuroscience 2010 11:13.)
CD11b, Monoclonal Antibody (Cat# AAA12146)
Full Name
MOUSE ANTI RAT CD11b:FITC
Gene Names
ITGAM; CD11B
Applications
Flow Cytometry
Pricing
IHC (Immunohistochemistry)
(At 1/100 staining Human pancreatic cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
p53, Polyclonal Antibody (Cat# AAA31355)
Full Name
Acetyl-p53 (Lys381) Antibody
Gene Names
TP53; P53; BCC7; LFS1; TRP53
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Rabbit (90%), Dog (100%)
Predicted Reactivity: Rabbit (90%), Dog (100%)
Applications
Western Blot, Immunohistochemistry, Peptide ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Pricing
Application Data
(At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
p53, Polyclonal Antibody (Cat# AAA31429)
Full Name
Phospho-p53 (Ser392) Antibody
Gene Names
TP53; P53; BCC7; LFS1; TRP53
Reactivity
Human, Mouse
Predicted Reactivity: Pig (88%), Bovine (88%), Sheep (88%), Rabbit (88%)
Predicted Reactivity: Pig (88%), Bovine (88%), Sheep (88%), Rabbit (88%)
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
ELISA
(Titration curve analysis of VISTA antibody to detect recombinant VISTA in ELISA at decreasing concentrations.)
VISTA, Monoclonal Antibody (Cat# AAA11015)
Full Name
VISTA Antibody [9E4]
Gene Names
VSIR; B7H5; GI24; B7-H5; PD-1H; SISP1; VISTA; PP2135; C10orf54; DD1alpha
Reactivity
Human
Applications
Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry
Purity
Protein A purified
Pricing
Application Data
(Published customer image Infiltration of GFP+ BM-cells in infarct and peri-infarct regions. (A-B) Dot plots of viable macrophages/granulocytes (CD11b+CD45high, top right quadrants) and microglia (CD11b+CD45dim, bottom right quadrants) in cortex from BM-chimeric unmanipulated mice and mice exposed to pMCAO. (C) Bar graph showing mean numbers of CD11b+CD45dim microglia and CD11b+CD45high macrophages/granulocytes in BM-chimeric mice 24 hours after pMCAO, subdivided based on expression of GFP (n = 5). Approximately 92% of of the CD45high population were GFP+. (D) Estimation and comparison of mean numbers of CD11b+CD45dim microglia in non-chimeric (n = 10) versus BM-chimeric mice (n = 5) 24 hours after of pMCAO shows significantly fewer CD11b+CD45dim microglial cells in irradiated mice. (E) Overview, showing distribution of infiltrating GFP+ BM-derived cells into infarct (IF) and peri-infarct (P-IF) regions 24 hours after pMCAO. (E-G) By 24 hours, GFP+ single cells (F) and vessel-associated aggregates of GFP+ cells (arrows in G) were observed in infarct and peri-infarct regions. Some of the vessel-associated cells were round, leukocyte-like cells (arrows) while others were elongated cells lining the vasculature (arrow heads in G and in insert). (H) Bar graph showing mean numbers of single GFP+ cells and vessel-associated aggregates of GFP+ cells in ipsi- and contralateral cortex 24 hours after surgery (n = 10). (I-P) Immunohistochemical staining of CD45.1 (I, K), CD45.2 (J, L), IgG2a (M, O) and CD45 (N, P) in ischemic tissue in BM-chimeric (I, J, M, N) and non-chimeric mice (K, L, O, P) 24 hours after pMCAO. N.D, none detected. Scale bars: 200 um (A), 10 um (B, C). 50 um (I-P) *P < 0.05, **P < 0.01, and ***P < 0.001.From: Clausen BH, Lambertsen KL, Babcock AA, Holm TH, Dagnaes-Hansen F, Finsen B. Interleukin-1beta and tumor necrosis factor-alpha are expressed by different subsets of microglia and macrophages after ischemic stroke in mice. J Neuroinflammation. 2008 Oct 23;5:46.)
CD11b, Monoclonal Antibody (Cat# AAA12184)
Full Name
RAT ANTI MOUSE CD11b
Gene Names
Itgam; CR3; CR3A; MAC1; Cd11b; Ly-40; Mac-1; Mac-1a; CD11b/CD18; F730045J24Rik
Applications
Immunohistochemistry, Flow Cytometry, Immunofluorescence, Immunoprecipitation
Pricing
Application Data
(Published customer image: Representative images of the inflammatory changes in the facial nucleus during axonal regeneration, one week following facial nerve transaction. a, b: CD11b immunoreactivity for microglia is increased in the axotomized facial nucleus, and microglia enwrap the facial motor neurons, e.g. at arrows. The regenerating neurons were retrogradely labelled with fluorogold. c, d: CD6- positive T-cells accumulated in the injured motor nucleus (arrows). They had little cytoplasm but dense nuclei (c) and were sometimes clustered around neurons retrogradely labelled with fluorogold (d). The scale bar in (a) also applies to (b) and that in (c) also applies to (d).From: Shokouhi et al. BMC Neuroscience 2010 11:13.)
CD11b, Monoclonal Antibody (Cat# AAA12045)
Full Name
MOUSE ANTI RAT CD11b:RPE
Gene Names
ITGAM; CD11B
Applications
Flow Cytometry
Pricing
Application Data
(Published customer image: Representative images of the inflammatory changes in the facial nucleus during axonal regeneration, one week following facial nerve transaction. a, b: CD11b immunoreactivity for microglia is increased in the axotomized facial nucleus, and microglia enwrap the facial motor neurons, e.g. at arrows. The regenerating neurons were retrogradely labelled with fluorogold. c, d: CD6- positive T-cells accumulated in the injured motor nucleus (arrows). They had little cytoplasm but dense nuclei (c) and were sometimes clustered around neurons retrogradely labelled with fluorogold (d). The scale bar in (a) also applies to (b) and that in (c) also applies to (d).From: Shokouhi et al. BMC Neuroscience 2010 11:13.)
CD11b, Monoclonal Antibody (Cat# AAA11969)
Full Name
MOUSE ANTI RAT CD11b
Gene Names
ITGAM; CD11B
Applications
Immunohistochemistry, Flow Cytometry, Immunofluorescence, Immunoprecipitation
Pricing
Application Data
(At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
LAT, Polyclonal Antibody (Cat# AAA31413)
Full Name
Phospho-LAT (Tyr161) Antibody
Gene Names
LAT; LAT1; pp36
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (91%), Zebrafish (83%), Bovine (100%), Horse (91%), Sheep (100%), Rabbit (80%), Dog (82%)
Predicted Reactivity: Pig (91%), Zebrafish (83%), Bovine (100%), Horse (91%), Sheep (100%), Rabbit (80%), Dog (82%)
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
Application Data
(Published customer image: Increased accumulation of repair-associated macrophages surrounding collaterals in ischemic hind limbs is PAR2-dependent. (A) Stainings of CD206-positive macrophages (green) and SMA-positive vessels (red) in non-ischemic (control) and ischemic (ligated) hind limbs of WT, PAR1-/- and PAR2-/- mice are shown. Nuclei were visualized with DAPI (blue). Arrows indicate single macrophages in the non-ischemic adductor. Quantification of the average number of repair-associated macrophages per vessel is indicated on the right. (B) Correlation between the number of CD206-positive macrophages in the ischemic tissues and the expression of CD11b and (C) CD115 on monocytes. ** p)
CD206, Monoclonal Antibody (Cat# AAA12123)
Full Name
RAT ANTI MOUSE CD206
Gene Names
Mrc1; MR; CD206; AW259686
Applications
Immunohistochemistry, Flow Cytometry, Immunofluorescence, Immunoprecipitation
Pricing
Application Data
(Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD86 antibody, clone 42F followed by horseradish peroxidase conjugated Goat anti Mouse IgG . Low power)
CD86, Monoclonal Antibody (Cat# AAA12226)
Full Name
MOUSE ANTI RAT CD86
Gene Names
CD86; B70; B7-2; B7.2; LAB72; CD28LG2
Applications
Immunohistochemistry, Flow Cytometry, Immunoprecipitation
Pricing
WB (Western Blot)
(Western blot analysis of PAK1 expression in Etoposide treated 293 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)
PAK1, Polyclonal Antibody (Cat# AAA31096)
Full Name
PAK1 Antibody
Gene Names
PAK1; PAKalpha
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
Application Data
(At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
CHK2, Polyclonal Antibody (Cat# AAA31398)
Full Name
Phospho-CHK2 (Ser33+Ser35) Antibody
Gene Names
CHEK2; CDS1; CHK2; LFS2; RAD53; hCds1; HuCds1; PP1425
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Bovine (82%)
Predicted Reactivity: Bovine (82%)
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
Application Data
(At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)
NFAT2, Polyclonal Antibody (Cat# AAA31428)
Full Name
Phospho-NFAT2 (Ser172) Antibody
Gene Names
NFATC1; NFAT2; NFATc; NF-ATC
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
Application Data
(Published customer image: Representative images of the inflammatory changes in the facial nucleus during axonal regeneration, one week following facial nerve transaction. a, b: CD11b immunoreactivity for microglia is increased in the axotomized facial nucleus, and microglia enwrap the facial motor neurons, e.g. at arrows. The regenerating neurons were retrogradely labelled with fluorogold. c, d: CD6- positive T-cells accumulated in the injured motor nucleus (arrows). They had little cytoplasm but dense nuclei (c) and were sometimes clustered around neurons retrogradely labelled with fluorogold (d). The scale bar in (a) also applies to (b) and that in (c) also applies to (d).From: Shokouhi et al. BMC Neuroscience 2010 11:13.)
CD11b, Monoclonal Antibody (Cat# AAA11971)
Full Name
MOUSE ANTI RAT CD11b
Gene Names
ITGAM; CD11B
Applications
Immunohistochemistry, Flow Cytometry, Immunofluorescence, Immunoprecipitation
Pricing
IHC (Immunohistochemistry)
(At 1/200 staining Human kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)
RhoA, Polyclonal Antibody (Cat# AAA31383)
Full Name
Phospho-RhoA (Ser188) Antibody
Gene Names
RHOA; ARHA; ARH12; RHO12; RHOH12
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Sheep (100%), Dog (100%)
Predicted Reactivity: Pig (100%), Bovine (100%), Sheep (100%), Dog (100%)
Applications
Western Blot, Immunohistochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
Application Data
(Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD86 antibody, clone 42F followed by horseradish peroxidase conjugated Goat anti Mouse IgG . Low power)
CD86, Monoclonal Antibody (Cat# AAA12229)
Full Name
MOUSE ANTI RAT CD86:FITC
Gene Names
CD86; B70; B7-2; B7.2; LAB72; CD28LG2
Applications
Flow Cytometry
Pricing
Application Data
(Published customer imageSLAMF3 blockade in human hepatocytes is associated with lower susceptibility to HCV. (A) SLAMF3 was stained in primary human hepatocytes (PHHs) and cells from the Huh-7 human hepatoma cell line with a specific antibody (HLy9.1.25 clone; grey) and an isotype-matched control (empty). One of four independent experiments is shown. Huh-7 cells were transfected with scrambled control (sc) siRNA or three specific siRNAs (#1, #2 and #3) targeting SLAMF3, prior to infection with HCVcc; siRNA efficiency was checked by quantifying SLAMF3 mRNA (B) and the CD81 expression level (C) by flow cytometry analysis at 48 h post-transfection. Results are presented as the mean +/-SD (n = 3). Intracellular viral RNA was quantified at 72 h p.i. (D) and the infection was measured at 72 h p.i. by focus-forming units FFUs counting (E) (as inhibition percent; mean of three independent experiments; error bars: SD. **p)
CD229, Monoclonal Antibody (Cat# AAA11951)
Full Name
MOUSE ANTI HUMAN CD229
Gene Names
LY9; hly9; mLY9; CD229; SLAMF3
Applications
Flow Cytometry, Immunoprecipitation
Pricing
Application Data
(Published customer image Infiltration of GFP+ BM-cells in infarct and peri-infarct regions. (A-B) Dot plots of viable macrophages/granulocytes (CD11b+CD45high, top right quadrants) and microglia (CD11b+CD45dim, bottom right quadrants) in cortex from BM-chimeric unmanipulated mice and mice exposed to pMCAO. (C) Bar graph showing mean numbers of CD11b+CD45dim microglia and CD11b+CD45high macrophages/granulocytes in BM-chimeric mice 24 hours after pMCAO, subdivided based on expression of GFP (n = 5). Approximately 92% of of the CD45high population were GFP+. (D) Estimation and comparison of mean numbers of CD11b+CD45dim microglia in non-chimeric (n = 10) versus BM-chimeric mice (n = 5) 24 hours after of pMCAO shows significantly fewer CD11b+CD45dim microglial cells in irradiated mice. (E) Overview, showing distribution of infiltrating GFP+ BM-derived cells into infarct (IF) and peri-infarct (P-IF) regions 24 hours after pMCAO. (E-G) By 24 hours, GFP+ single cells (F) and vessel-associated aggregates of GFP+ cells (arrows in G) were observed in infarct and peri-infarct regions. Some of the vessel-associated cells were round, leukocyte-like cells (arrows) while others were elongated cells lining the vasculature (arrow heads in G and in insert). (H) Bar graph showing mean numbers of single GFP+ cells and vessel-associated aggregates of GFP+ cells in ipsi- and contralateral cortex 24 hours after surgery (n = 10). (I-P) Immunohistochemical staining of CD45.1 (I, K), CD45.2 (J, L), IgG2a (M, O) and CD45 (N, P) in ischemic tissue in BM-chimeric (I, J, M, N) and non-chimeric mice (K, L, O, P) 24 hours after pMCAO. N.D, none detected. Scale bars: 200 um (A), 10 um (B, C). 50 um (I-P) *P < 0.05, **P < 0.01, and ***P < 0.001.From: Clausen BH, Lambertsen KL, Babcock AA, Holm TH, Dagnaes-Hansen F, Finsen B. Interleukin-1beta and tumor necrosis factor-alpha are expressed by different subsets of microglia and macrophages after ischemic stroke in mice. J Neuroinflammation. 2008 Oct 23;5:46.)
CD11b, Monoclonal Antibody (Cat# AAA12185)
Full Name
RAT ANTI MOUSE CD11b
Gene Names
Itgam; CR3; CR3A; MAC1; Cd11b; Ly-40; Mac-1; Mac-1a; CD11b/CD18; F730045J24Rik
Applications
Immunohistochemistry, Flow Cytometry, Immunofluorescence, Immunoprecipitation
Pricing
Application Data
(Published customer image Infiltration of GFP+ BM-cells in infarct and peri-infarct regions. (A-B) Dot plots of viable macrophages/granulocytes (CD11b+CD45high, top right quadrants) and microglia (CD11b+CD45dim, bottom right quadrants) in cortex from BM-chimeric unmanipulated mice and mice exposed to pMCAO. (C) Bar graph showing mean numbers of CD11b+CD45dim microglia and CD11b+CD45high macrophages/granulocytes in BM-chimeric mice 24 hours after pMCAO, subdivided based on expression of GFP (n = 5). Approximately 92% of of the CD45high population were GFP+. (D) Estimation and comparison of mean numbers of CD11b+CD45dim microglia in non-chimeric (n = 10) versus BM-chimeric mice (n = 5) 24 hours after of pMCAO shows significantly fewer CD11b+CD45dim microglial cells in irradiated mice. (E) Overview, showing distribution of infiltrating GFP+ BM-derived cells into infarct (IF) and peri-infarct (P-IF) regions 24 hours after pMCAO. (E-G) By 24 hours, GFP+ single cells (F) and vessel-associated aggregates of GFP+ cells (arrows in G) were observed in infarct and peri-infarct regions. Some of the vessel-associated cells were round, leukocyte-like cells (arrows) while others were elongated cells lining the vasculature (arrow heads in G and in insert). (H) Bar graph showing mean numbers of single GFP+ cells and vessel-associated aggregates of GFP+ cells in ipsi- and contralateral cortex 24 hours after surgery (n = 10). (I-P) Immunohistochemical staining of CD45.1 (I, K), CD45.2 (J, L), IgG2a (M, O) and CD45 (N, P) in ischemic tissue in BM-chimeric (I, J, M, N) and non-chimeric mice (K, L, O, P) 24 hours after pMCAO. N.D, none detected. Scale bars: 200 um (A), 10 um (B, C). 50 um (I-P) *P < 0.05, **P < 0.01, and ***P < 0.001.From: Clausen BH, Lambertsen KL, Babcock AA, Holm TH, Dagnaes-Hansen F, Finsen B. Interleukin-1beta and tumor necrosis factor-alpha are expressed by different subsets of microglia and macrophages after ischemic stroke in mice. J Neuroinflammation. 2008 Oct 23;5:46.)
CD11b, Monoclonal Antibody (Cat# AAA12183)
Full Name
RAT ANTI MOUSE CD11b:FITC
Gene Names
Itgam; CR3; CR3A; MAC1; Cd11b; Ly-40; Mac-1; Mac-1a; CD11b/CD18; F730045J24Rik
Applications
Flow Cytometry
Pricing
Application Data
(Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD163 antibody, clone EDHu-1 followed by the Histar detection system . Low power)
CD163, Monoclonal Antibody (Cat# AAA12100)
Full Name
MOUSE ANTI HUMAN CD163
Gene Names
CD163; M130; MM130
Applications
Immunohistochemistry, Flow Cytometry, Immunofluorescence, Immunoassay, Immunohistochemistry, Western Blot
Pricing
Application Data
(Published customer image: Representative images of the inflammatory changes in the facial nucleus during axonal regeneration, one week following facial nerve transaction. a, b: CD11b immunoreactivity for microglia is increased in the axotomized facial nucleus, and microglia enwrap the facial motor neurons, e.g. at arrows. The regenerating neurons were retrogradely labelled with fluorogold. c, d: CD6- positive T-cells accumulated in the injured motor nucleus (arrows). They had little cytoplasm but dense nuclei (c) and were sometimes clustered around neurons retrogradely labelled with fluorogold (d). The scale bar in (a) also applies to (b) and that in (c) also applies to (d).From: Shokouhi et al. BMC Neuroscience 2010 11:13.)
CD11b, Monoclonal Antibody (Cat# AAA11876)
Full Name
MOUSE ANTI RAT CD11b:FITC
Gene Names
ITGAM; CD11B
Applications
Flow Cytometry
Pricing
Application Data
(Analysis of Protein Array containing more than 19, 000 full-length human proteins using Oct-2 Mouse Monoclonal Antibody (OCT2/2137) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
OCT-2 (POU2F2), Monoclonal Antibody (Cat# AAA23902)
Full Name
OCT-2 (POU2F2) (B-Cell Marker)
Gene Names
POU2F2; OCT2; OTF2; Oct-2
Reactivity
Human. Others not known.
Applications
Western Blot, Immunohistochemistry
Pricing
ELISA
(Titration curve analysis of VISTA antibody to detect recombinant VISTA in ELISA at decreasing concentrations.)
VISTA, Monoclonal Antibody (Cat# AAA11014)
Full Name
VISTA Antibody [8E11]
Gene Names
VSIR; B7H5; GI24; B7-H5; PD-1H; SISP1; VISTA; PP2135; C10orf54; DD1alpha
Reactivity
Human
Applications
Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry
Purity
Protein A purified
Pricing
Application Data
(Published customer image: Increased accumulation of repair-associated macrophages surrounding collaterals in ischemic hind limbs is PAR2-dependent. (A) Stainings of CD206-positive macrophages (green) and SMA-positive vessels (red) in non-ischemic (control) and ischemic (ligated) hind limbs of WT, PAR1-/- and PAR2-/- mice are shown. Nuclei were visualized with DAPI (blue). Arrows indicate single macrophages in the non-ischemic adductor. Quantification of the average number of repair-associated macrophages per vessel is indicated on the right. (B) Correlation between the number of CD206-positive macrophages in the ischemic tissues and the expression of CD11b and (C) CD115 on monocytes. ** p)
CD206, Monoclonal Antibody (Cat# AAA12118)
Full Name
RAT ANTI MOUSE CD206:Biotin
Gene Names
Mrc1; MR; CD206; AW259686
Applications
Flow Cytometry
Pricing
ELISA
(Titration curve analysis of VISTA antibody to detect recombinant VISTA in ELISA at decreasing concentrations.)
VISTA, Monoclonal Antibody (Cat# AAA11012)
Full Name
VISTA Antibody [4C4]
Gene Names
VSIR; B7H5; GI24; B7-H5; PD-1H; SISP1; VISTA; PP2135; C10orf54; DD1alpha
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry
Purity
Protein A purified
Pricing
IHC (Immunohistochemistry)
(At 1/100 staining Human colorectal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
CD227/MUC1, Polyclonal Antibody (Cat# AAA31426)
Full Name
Phospho-CD227/MUC1 (Ser1227) Antibody
Gene Names
MUC1; EMA; PEM; PUM; KL-6; MAM6; PEMT; CD227; H23AG; MUC-1; CA 15-3; MUC-1/X; MUC1/ZD; MUC-1/SEC
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
Application Data
(Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD25 followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 as a detection reagent. High power)
CD25, Monoclonal Antibody (Cat# AAA11965)
Full Name
MOUSE ANTI RAT CD25
Gene Names
Il2ra; IL2RAC
Applications
Immunohistochemistry, Flow Cytometry, Immunoprecipitation, Immunohistochemistry
Pricing
WB (Western Blot)
(Western Blot analysis using AAA14675 (1:1000)Lane 1: Human Interleukin 23, p40 fusion proteinLane 2: Human Interleukin 23, p19 fusion protein. Lane 3: Recombinant human single chain Interleukin 23 (DYKDDDDK-tagged))
Interleukin 23, p40, Monoclonal Antibody (Cat# AAA14675)
Full Name
Interleukin 23, p40 (IL-23)
Gene Names
IL23A; P19; SGRF; IL-23; IL-23A; IL23P19
Reactivity
Human
Applications
Western Blot
Purity
Purified
Purified
Purified
Pricing
Application Data
(Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD25 followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 as a detection reagent. High power)
CD25, Monoclonal Antibody (Cat# AAA11966)
Full Name
MOUSE ANTI RAT CD25
Gene Names
Il2ra; IL2RAC
Applications
Immunohistochemistry, Flow Cytometry, Immunoprecipitation, Immunohistochemistry
Pricing
Application Data
(Published customer image: Representative images of the inflammatory changes in the facial nucleus during axonal regeneration, one week following facial nerve transaction. a, b: CD11b immunoreactivity for microglia is increased in the axotomized facial nucleus, and microglia enwrap the facial motor neurons, e.g. at arrows. The regenerating neurons were retrogradely labelled with fluorogold. c, d: CD6- positive T-cells accumulated in the injured motor nucleus (arrows). They had little cytoplasm but dense nuclei (c) and were sometimes clustered around neurons retrogradely labelled with fluorogold (d). The scale bar in (a) also applies to (b) and that in (c) also applies to (d).From: Shokouhi et al. BMC Neuroscience 2010 11:13.)
CD11b, Monoclonal Antibody (Cat# AAA11970)
Full Name
MOUSE ANTI RAT CD11b
Gene Names
ITGAM; CD11B
Applications
Immunohistochemistry, Flow Cytometry, Immunofluorescence, Immunoprecipitation
Pricing
Application Data
(Published customer image: Increased accumulation of repair-associated macrophages surrounding collaterals in ischemic hind limbs is PAR2-dependent. (A) Stainings of CD206-positive macrophages (green) and SMA-positive vessels (red) in non-ischemic (control) and ischemic (ligated) hind limbs of WT, PAR1-/- and PAR2-/- mice are shown. Nuclei were visualized with DAPI (blue). Arrows indicate single macrophages in the non-ischemic adductor. Quantification of the average number of repair-associated macrophages per vessel is indicated on the right. (B) Correlation between the number of CD206-positive macrophages in the ischemic tissues and the expression of CD11b and (C) CD115 on monocytes. ** p)
CD206, Monoclonal Antibody (Cat# AAA12124)
Full Name
RAT ANTI MOUSE CD206:RPE
Gene Names
Mrc1; MR; CD206; AW259686
Applications
Flow Cytometry
Pricing
Standard Curve (Sample)
chemokine (C-C motif) ligand 17, ELISA Kit (Cat# AAA18087)
Full Name
Monkey C-C motif chemokine 17, CCL17 ELISA Kit
Reactivity
Monkey
Pricing
ELISA
(Titration curve analysis of VISTA antibody to detect recombinant VISTA in ELISA at decreasing concentrations.)
VISTA, Monoclonal Antibody (Cat# AAA11013)
Full Name
VISTA Antibody [6D2]
Gene Names
VSIR; B7H5; GI24; B7-H5; PD-1H; SISP1; VISTA; PP2135; C10orf54; DD1alpha
Reactivity
Human
Applications
Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry
Purity
Protein A purified
Pricing
Application Data
(Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD25 followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 as a detection reagent. High power)
CD25, Monoclonal Antibody (Cat# AAA12044)
Full Name
MOUSE ANTI RAT CD25:RPE
Gene Names
Il2ra; IL2RAC
Applications
Flow Cytometry
Pricing