Filters
Clonality
Type
Reactivity
Gene Name
Isotype
Host
Application
Clone
53 results for " Kidney Development" - showing 1-50
IHC (Immunohistchemistry)
(At 1/100 staining Human colorectal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
E-cadherin, Polyclonal Antibody (Cat# AAA31444)
Full Name
Phospho-E-cadherin (Ser844) Antibody
Gene Names
CDH1; UVO; CDHE; ECAD; LCAM; Arc-1; CD324
Reactivity
Human, Mouse, Rat, Monkey
Predicted Reactivity: Pig (100%), Bovine (91%), Horse (91%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (83%), Xenopus (100%)
Predicted Reactivity: Pig (100%), Bovine (91%), Horse (91%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (83%), Xenopus (100%)
Applications
Western Blot, Immunohistochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
FCM (Flow Cytometry)
(HAS2 Antibody (Center) (AAA28714) flow cytometric analysis of K562 cells (bottom histogram) compared to a Rabbit IgG Isotype control (negative control-top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
HAS2, Polyclonal Antibody (Cat# AAA28714)
Full Name
HAS2 Antibody (Center)
Reactivity
Human, mouse
Applications
Western Blot, Immunohistochemistry, Flow Cytometry
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
WB (Western Blot)
(CDADC1 monoclonal antibody. Western Blot analysis of CDADC1 expression in NIH/3T3.)
CDADC1, Monoclonal Antibody (Cat# AAA24752)
Full Name
CDADC1 (Cytidine and dCMP Deaminase Domain-containing Protein 1, Testis Development Protein NYD-SP15, MGC150615, MGC41774, MGC57136, NYD-SP15, BA103J18.1) (Biotin)
Gene Names
CDADC1; NYD-SP15; bA103J18.1
Reactivity
Human, Mouse
Applications
EIA, IHC, WB
Purity
Purified by Protein A Affinity Chromatography.
Pricing
WB (Western Blot)
(CDADC1 monoclonal antibody. Western Blot analysis of CDADC1 expression in NIH/3T3.)
CDADC1, Monoclonal Antibody (Cat# AAA24102)
Full Name
CDADC1 (Cytidine and dCMP Deaminase Domain-containing Protein 1, Testis Development Protein NYD-SP15, MGC150615, MGC41774, MGC57136, NYD-SP15, BA103J18.1)
Gene Names
CDADC1; NYD-SP15; bA103J18.1; RP11-103J18.2
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry
Purity
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Pricing
WB (Western Blot)
(CDADC1 monoclonal antibody. Western Blot analysis of CDADC1 expression in NIH/3T3.)
CDADC1, Monoclonal Antibody (Cat# AAA25638)
Full Name
CDADC1 (Cytidine and dCMP Deaminase Domain-containing Protein 1, Testis Development Protein NYD-SP15, MGC150615, MGC41774, MGC57136, NYD-SP15, BA103J18.1) (PE)
Gene Names
CDADC1; NYD-SP15; bA103J18.1
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Pricing
WB (Western Blot)
(CDADC1 monoclonal antibody. Western Blot analysis of CDADC1 expression in NIH/3T3.)
CDADC1, Monoclonal Antibody (Cat# AAA24162)
Full Name
CDADC1 (Cytidine and dCMP Deaminase Domain-containing Protein 1, Testis Development Protein NYD-SP15, MGC150615, MGC41774, MGC57136, NYD-SP15, BA103J18.1) (AP)
Gene Names
CDADC1; NYD-SP15; bA103J18.1
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Pricing
WB (Western Blot)
(CDADC1 monoclonal antibody. Western Blot analysis of CDADC1 expression in NIH/3T3.)
CDADC1, Monoclonal Antibody (Cat# AAA25049)
Full Name
CDADC1 (Cytidine and dCMP Deaminase Domain-containing Protein 1, Testis Development Protein NYD-SP15, MGC150615, MGC41774, MGC57136, NYD-SP15, BA103J18.1) (FITC)
Gene Names
CDADC1; NYD-SP15; bA103J18.1
Reactivity
Human, Mouse
Applications
EIA, IHC, WB
Purity
Purified by Protein A Affinity Chromatography.
Pricing
WB (Western Blot)
(CDADC1 monoclonal antibody. Western Blot analysis of CDADC1 expression in NIH/3T3.)
CDADC1, Monoclonal Antibody (Cat# AAA24457)
Full Name
CDADC1 (Cytidine and dCMP Deaminase Domain-containing Protein 1, Testis Development Protein NYD-SP15, MGC150615, MGC41774, MGC57136, NYD-SP15, BA103J18.1) APC
Gene Names
CDADC1; NYD-SP15; bA103J18.1
Reactivity
Human, Mouse
Applications
EIA, IHC, WB
Purity
Purified by Protein A Affinity Chromatography.
Pricing
IHC (Immunohistchemistry)
(Figure 6. IHC analysis of Thrombopoietin using anti- Thrombopoietin antibody (AAA19166).Thrombopoietin was detected in paraffin-embedded section of rat kidney tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti- Thrombopoietin Antibody (AAA19166) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
Thrombopoietin, Polyclonal Antibody (Cat# AAA19166)
Full Name
Anti-Thrombopoietin Picoband Antibody
Gene Names
Thpo; Ml; Tpo; Mgdf; Mpllg
Reactivity
Mouse, Rat
No cross reactivity with other proteins
No cross reactivity with other proteins
Applications
EIA, IHC, WB
Purity
Immunogen affinity purified
Pricing
SDS-PAGE
(SDS-PAGE Analysis Purified Podocalyxin Mouse Monoclonal Antibody (3D3). Confirmation of Integrity and Purity of Antibody.)
Podocalyxin (PODXL), Monoclonal Antibody (Cat# AAA13811)
Full Name
Podocalyxin (PODXL) (Hematopoietic Stem Cell Marker) Mouse Monoclonal Antibody
Gene Names
PODXL; PC; PCLP; Gp200; PCLP-1
Reactivity
Human, Rabbit, Rat
Applications
Flow Cytometry, Immunofluorescence, Immunoprecipitation, Western Blot, Immunohistochemistry
Pricing
IP (Immunoprecipitation)
(Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 11H10. Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts of (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degree C and next incubated with 250ug of MEF lysate for 2 hours at 4 degree C.)
VPS35, Monoclonal Antibody (Cat# AAA27707)
Full Name
VPS35 Antibody, Clone 11H10: PerCP
Gene Names
VPS35; MEM3; PARK17
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunoprecipitation
Purity
Protein G Purified
Pricing
IP (Immunoprecipitation)
(Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 8A3. Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts of (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degree C and next incubated with 250ug of MEF lysate for 2 hours at 4 degree C.)
VPS35, Monoclonal Antibody (Cat# AAA27689)
Full Name
VPS35 Antibody, Clone 8A3: PerCP
Gene Names
VPS35; MEM3; PARK17
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
Purity
Protein G Purified
Pricing
IP (Immunoprecipitation)
(Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 8A3. Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts of (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degree C and next incubated with 250ug of MEF lysate for 2 hours at 4 degree C.)
VPS35, Monoclonal Antibody (Cat# AAA27682)
Full Name
VPS35 Antibody, Clone 8A3
Gene Names
VPS35; MEM3; PARK17
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
Purity
Protein G Purified
Pricing
IP (Immunoprecipitation)
(Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 11H10. Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts of (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degree C and next incubated with 250ug of MEF lysate for 2 hours at 4 degree C.)
VPS35, Monoclonal Antibody (Cat# AAA27705)
Full Name
VPS35 Antibody, Clone 11H10: FITC
Gene Names
VPS35; MEM3; PARK17
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunoprecipitation
Purity
Protein G Purified
Pricing
IP (Immunoprecipitation)
(Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 8A3. Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts of (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degree C and next incubated with 250ug of MEF lysate for 2 hours at 4 degree C.)
VPS35, Monoclonal Antibody (Cat# AAA27685)
Full Name
VPS35 Antibody, Clone 8A3: ATTO 594
Gene Names
VPS35; MEM3; PARK17
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
Purity
Protein G Purified
Pricing
Application Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for the detection of V5 tagged WEEV_nsP3 protein by western blotting and immunofluorescenceImage caption: WEEV nsP3 interaction with host IKKbeta. A) U87MGs were transfected in a 6-well plate with 5 ug of pUC19 and WEEV_nsP3_HA for 24 hours. Cell lysates were resolved using SDS-PAGE and subsequently immunoblotted with V5 antibody and beta-actin served as a loading control. B) U87MGs were transfected with WEEV_nsP3_V5; cells were fixed after 24 hours and stained with antibodies against the endogenous IKKbeta and the V5 tag. Cells were incubated with appropriate secondary Alexa Fluor antibodies and the nuclei stained with DAPI. Co-localization of IKKbeta with WEEV_nsP3_V5 (yellow) was observed as shown by the arrows. B) Panels E -H serve as an example of transfected cells in a given field of view that show co-localization of IKKbeta and WEEV_nsP3_V5 24 hours post transfection. Panels I-L represent magnified images of other cells showing co-localization of IKKbeta and WEEV_nsP3_V5. Panel M is a magnified image of panel L. The co-localization was confirmed by Z-stack analysis. Co-localization was calculated to be approximately in 61% of cells (163 cells were counted of which 44% demonstrated expression of nsP3. Of those cells that expressed nsP3, 61% showed co-localization of both proteins). Images were taken using Nikon Eclipse TE2000-U at 60x magnification and are representative of 2 independent experiments.From: Amaya M, Voss K, Sampey G, Senina S, de la Fuente C, et al. (2014) The Role of IKKbeta in Venezuelan Equine Encephalitis Virus Infection. PLoS ONE 9(2): e86745.)
V5-TAG, Monoclonal Antibody (Cat# AAA12081)
Full Name
MOUSE ANTI V5-TAG:HRP
Applications
Western Blot
Pricing
IP (Immunoprecipitation)
(Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 11H10. Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts of (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degree C and next incubated with 250ug of MEF lysate for 2 hours at 4 degree C.)
VPS35, Monoclonal Antibody (Cat# AAA27704)
Full Name
VPS35 Antibody, Clone 11H10: Biotin
Gene Names
VPS35; MEM3; PARK17
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunoprecipitation
Purity
Protein G Purified
Pricing
IP (Immunoprecipitation)
(Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 11H10. Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts of (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degree C and next incubated with 250ug of MEF lysate for 2 hours at 4 degree C.)
VPS35, Monoclonal Antibody (Cat# AAA27703)
Full Name
VPS35 Antibody, Clone 11H10: ATTO 594
Gene Names
VPS35; MEM3; PARK17
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunoprecipitation
Purity
Protein G Purified
Pricing
IP (Immunoprecipitation)
(Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 7E4. Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degree C and next incubated with 250ug of MEF lysate for 2 hours at 4 degree C.)
VPS35, Monoclonal Antibody (Cat# AAA27671)
Full Name
VPS35 Antibody, Clone 7E4: PerCP
Gene Names
VPS35; MEM3; PARK17
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
Purity
Protein G Purified
Pricing
IP (Immunoprecipitation)
(Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 5A9. Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts of (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degree C and next incubated with 250ug of MEF lysate for 2 hours at 4 degree C.)
VPS35, Monoclonal Antibody (Cat# AAA27680)
Full Name
VPS35 Antibody, Clone 5A9: PerCP
Gene Names
VPS35; MEM3; PARK17
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
Purity
Protein G Purified
Pricing
Application Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for the detection of V5 tagged WEEV_nsP3 protein by western blotting and immunofluorescenceImage caption: WEEV nsP3 interaction with host IKKbeta. A) U87MGs were transfected in a 6-well plate with 5 ug of pUC19 and WEEV_nsP3_HA for 24 hours. Cell lysates were resolved using SDS-PAGE and subsequently immunoblotted with V5 antibody and beta-actin served as a loading control. B) U87MGs were transfected with WEEV_nsP3_V5; cells were fixed after 24 hours and stained with antibodies against the endogenous IKKbeta and the V5 tag. Cells were incubated with appropriate secondary Alexa Fluor antibodies and the nuclei stained with DAPI. Co-localization of IKKbeta with WEEV_nsP3_V5 (yellow) was observed as shown by the arrows. B) Panels E -H serve as an example of transfected cells in a given field of view that show co-localization of IKKbeta and WEEV_nsP3_V5 24 hours post transfection. Panels I-L represent magnified images of other cells showing co-localization of IKKbeta and WEEV_nsP3_V5. Panel M is a magnified image of panel L. The co-localization was confirmed by Z-stack analysis. Co-localization was calculated to be approximately in 61% of cells (163 cells were counted of which 44% demonstrated expression of nsP3. Of those cells that expressed nsP3, 61% showed co-localization of both proteins). Images were taken using Nikon Eclipse TE2000-U at 60x magnification and are representative of 2 independent experiments.From: Amaya M, Voss K, Sampey G, Senina S, de la Fuente C, et al. (2014) The Role of IKKbeta in Venezuelan Equine Encephalitis Virus Infection. PLoS ONE 9(2): e86745.)
V5-TAG, Monoclonal Antibody (Cat# AAA11864)
Full Name
MOUSE ANTI V5-TAG:FITC
Applications
Immunofluorescence
Pricing
Application Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for the detection of V5 tagged WEEV_nsP3 protein by western blotting and immunofluorescenceImage caption: WEEV nsP3 interaction with host IKKbeta. A) U87MGs were transfected in a 6-well plate with 5 ug of pUC19 and WEEV_nsP3_HA for 24 hours. Cell lysates were resolved using SDS-PAGE and subsequently immunoblotted with V5 antibody and beta-actin served as a loading control. B) U87MGs were transfected with WEEV_nsP3_V5; cells were fixed after 24 hours and stained with antibodies against the endogenous IKKbeta and the V5 tag. Cells were incubated with appropriate secondary Alexa Fluor antibodies and the nuclei stained with DAPI. Co-localization of IKKbeta with WEEV_nsP3_V5 (yellow) was observed as shown by the arrows. B) Panels E -H serve as an example of transfected cells in a given field of view that show co-localization of IKKbeta and WEEV_nsP3_V5 24 hours post transfection. Panels I-L represent magnified images of other cells showing co-localization of IKKbeta and WEEV_nsP3_V5. Panel M is a magnified image of panel L. The co-localization was confirmed by Z-stack analysis. Co-localization was calculated to be approximately in 61% of cells (163 cells were counted of which 44% demonstrated expression of nsP3. Of those cells that expressed nsP3, 61% showed co-localization of both proteins). Images were taken using Nikon Eclipse TE2000-U at 60x magnification and are representative of 2 independent experiments.From: Amaya M, Voss K, Sampey G, Senina S, de la Fuente C, et al. (2014) The Role of IKKbeta in Venezuelan Equine Encephalitis Virus Infection. PLoS ONE 9(2): e86745.)
V5-TAG, Monoclonal Antibody (Cat# AAA11930)
Full Name
MOUSE ANTI V5-TAG
Applications
Immunohistochemistry, Flow Cytometry, Immunofluorescence, Immunoprecipitation, Western Blot, Radioimmunoassay
Pricing
IP (Immunoprecipitation)
(Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 11H10. Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts of (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degree C and next incubated with 250ug of MEF lysate for 2 hours at 4 degree C.)
VPS35, Monoclonal Antibody (Cat# AAA27700)
Full Name
VPS35 Antibody, Clone 11H10
Gene Names
VPS35; MEM3; PARK17
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunoprecipitation
Purity
Protein G Purified
Pricing
IP (Immunoprecipitation)
(Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 11H10. Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts of (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degree C and next incubated with 250ug of MEF lysate for 2 hours at 4 degree C.)
VPS35, Monoclonal Antibody (Cat# AAA27702)
Full Name
VPS35 Antibody, Clone 11H10: ATTO 488
Gene Names
VPS35; MEM3; PARK17
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunoprecipitation
Purity
Protein G Purified
Pricing
IP (Immunoprecipitation)
(Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 8A3. Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts of (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degree C and next incubated with 250ug of MEF lysate for 2 hours at 4 degree C.)
VPS35, Monoclonal Antibody (Cat# AAA27687)
Full Name
VPS35 Antibody, Clone 8A3: FITC
Gene Names
VPS35; MEM3; PARK17
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
Purity
Protein G Purified
Pricing
IP (Immunoprecipitation)
(Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 8A3. Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts of (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degree C and next incubated with 250ug of MEF lysate for 2 hours at 4 degree C.)
VPS35, Monoclonal Antibody (Cat# AAA27683)
Full Name
VPS35 Antibody, Clone 8A3: ATTO 390
Gene Names
VPS35; MEM3; PARK17
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
Purity
Protein G Purified
Pricing
Application Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for the detection of V5 tagged WEEV_nsP3 protein by western blotting and immunofluorescenceImage caption: WEEV nsP3 interaction with host IKKbeta. A) U87MGs were transfected in a 6-well plate with 5 ug of pUC19 and WEEV_nsP3_HA for 24 hours. Cell lysates were resolved using SDS-PAGE and subsequently immunoblotted with V5 antibody and beta-actin served as a loading control. B) U87MGs were transfected with WEEV_nsP3_V5; cells were fixed after 24 hours and stained with antibodies against the endogenous IKKbeta and the V5 tag. Cells were incubated with appropriate secondary Alexa Fluor antibodies and the nuclei stained with DAPI. Co-localization of IKKbeta with WEEV_nsP3_V5 (yellow) was observed as shown by the arrows. B) Panels E -H serve as an example of transfected cells in a given field of view that show co-localization of IKKbeta and WEEV_nsP3_V5 24 hours post transfection. Panels I-L represent magnified images of other cells showing co-localization of IKKbeta and WEEV_nsP3_V5. Panel M is a magnified image of panel L. The co-localization was confirmed by Z-stack analysis. Co-localization was calculated to be approximately in 61% of cells (163 cells were counted of which 44% demonstrated expression of nsP3. Of those cells that expressed nsP3, 61% showed co-localization of both proteins). Images were taken using Nikon Eclipse TE2000-U at 60x magnification and are representative of 2 independent experiments.From: Amaya M, Voss K, Sampey G, Senina S, de la Fuente C, et al. (2014) The Role of IKKbeta in Venezuelan Equine Encephalitis Virus Infection. PLoS ONE 9(2): e86745.)
V5-TAG, Monoclonal Antibody (Cat# AAA12211)
Full Name
MOUSE ANTI V5-TAG
Applications
Immunohistochemistry, Flow Cytometry, Immunofluorescence, Immunoprecipitation, Western Blot, Radioimmunoassay
Pricing
WB (Western Blot)
(Western Blot using anti-polysialic acid antibody Rat brain lysate was resolved on a 10% SDS PAGE gel and blots probed with at 2 µg/ml before being detected by a secondary antibody.)
Polysialic acid, Recombinant Antibody (Cat# AAA14183)
Full Name
Anti-Polysialic acid [735]
Reactivity
Human, Mouse, Rat
Applications
Immunoblot, Immunoprecipitation, Western Blot, Flow Cytometry, Immunohistochemistry
Purity
Protein A Affinity Purified
Pricing
IP (Immunoprecipitation)
(Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 11H10. Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts of (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degree C and next incubated with 250ug of MEF lysate for 2 hours at 4 degree C.)
VPS35, Monoclonal Antibody (Cat# AAA27706)
Full Name
VPS35 Antibody, Clone 11H10: HRP
Gene Names
VPS35; MEM3; PARK17
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunoprecipitation
Purity
Protein G Purified
Pricing
IP (Immunoprecipitation)
(Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 11H10. Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts of (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degree C and next incubated with 250ug of MEF lysate for 2 hours at 4 degree C.)
VPS35, Monoclonal Antibody (Cat# AAA27708)
Full Name
VPS35 Antibody, Clone 11H10: RPE
Gene Names
VPS35; MEM3; PARK17
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunoprecipitation
Purity
Protein G Purified
Pricing
IP (Immunoprecipitation)
(Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 11H10. Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts of (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degree C and next incubated with 250ug of MEF lysate for 2 hours at 4 degree C.)
VPS35, Monoclonal Antibody (Cat# AAA27701)
Full Name
VPS35 Antibody, Clone 11H10: ATTO 390
Gene Names
VPS35; MEM3; PARK17
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunoprecipitation
Purity
Protein G Purified
Pricing
IP (Immunoprecipitation)
(Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 5A9. Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts of (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degree C and next incubated with 250ug of MEF lysate for 2 hours at 4 degree C.)
VPS35, Monoclonal Antibody (Cat# AAA27677)
Full Name
VPS35 Antibody, Clone 5A9: Biotin
Gene Names
VPS35; MEM3; PARK17
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
Purity
Protein G Purified
Pricing
IP (Immunoprecipitation)
(Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 5A9. Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts of (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degree C and next incubated with 250ug of MEF lysate for 2 hours at 4 degree C.)
VPS35, Monoclonal Antibody (Cat# AAA27678)
Full Name
VPS35 Antibody, Clone 5A9: FITC
Gene Names
VPS35; MEM3; PARK17
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
Purity
Protein G Purified
Pricing
IP (Immunoprecipitation)
(Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 5A9. Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts of (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degree C and next incubated with 250ug of MEF lysate for 2 hours at 4 degree C.)
VPS35, Monoclonal Antibody (Cat# AAA27674)
Full Name
VPS35 Antibody, Clone 5A9: ATTO 390
Gene Names
VPS35; MEM3; PARK17
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
Purity
Protein G Purified
Pricing
Application Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for the detection of V5 tagged WEEV_nsP3 protein by western blotting and immunofluorescenceImage caption: WEEV nsP3 interaction with host IKKbeta. A) U87MGs were transfected in a 6-well plate with 5 ug of pUC19 and WEEV_nsP3_HA for 24 hours. Cell lysates were resolved using SDS-PAGE and subsequently immunoblotted with V5 antibody and beta-actin served as a loading control. B) U87MGs were transfected with WEEV_nsP3_V5; cells were fixed after 24 hours and stained with antibodies against the endogenous IKKbeta and the V5 tag. Cells were incubated with appropriate secondary Alexa Fluor antibodies and the nuclei stained with DAPI. Co-localization of IKKbeta with WEEV_nsP3_V5 (yellow) was observed as shown by the arrows. B) Panels E -H serve as an example of transfected cells in a given field of view that show co-localization of IKKbeta and WEEV_nsP3_V5 24 hours post transfection. Panels I-L represent magnified images of other cells showing co-localization of IKKbeta and WEEV_nsP3_V5. Panel M is a magnified image of panel L. The co-localization was confirmed by Z-stack analysis. Co-localization was calculated to be approximately in 61% of cells (163 cells were counted of which 44% demonstrated expression of nsP3. Of those cells that expressed nsP3, 61% showed co-localization of both proteins). Images were taken using Nikon Eclipse TE2000-U at 60x magnification and are representative of 2 independent experiments.From: Amaya M, Voss K, Sampey G, Senina S, de la Fuente C, et al. (2014) The Role of IKKbeta in Venezuelan Equine Encephalitis Virus Infection. PLoS ONE 9(2): e86745.)
V5-TAG, Monoclonal Antibody (Cat# AAA11850)
Full Name
MOUSE ANTI V5-TAG:Biotin
Applications
Immunohistochemistry, Western Blot
Pricing
IP (Immunoprecipitation)
(Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 5A9. Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts of (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degree C and next incubated with 250ug of MEF lysate for 2 hours at 4 degree C.)
VPS35, Monoclonal Antibody (Cat# AAA27681)
Full Name
VPS35 Antibody, Clone 5A9: RPE
Gene Names
VPS35; MEM3; PARK17
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
Purity
Protein G Purified
Pricing
IP (Immunoprecipitation)
(Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 7E4. Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degree C and next incubated with 250ug of MEF lysate for 2 hours at 4 degree C.)
VPS35, Monoclonal Antibody (Cat# AAA27670)
Full Name
VPS35 Antibody, Clone 7E4: HRP
Gene Names
VPS35; MEM3; PARK17
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
Purity
Protein G Purified
Pricing
IP (Immunoprecipitation)
(Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 8A3. Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts of (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degree C and next incubated with 250ug of MEF lysate for 2 hours at 4 degree C.)
VPS35, Monoclonal Antibody (Cat# AAA27688)
Full Name
VPS35 Antibody, Clone 8A3: HRP
Gene Names
VPS35; MEM3; PARK17
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
Purity
Protein G Purified
Pricing
IP (Immunoprecipitation)
(Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 8A3. Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts of (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degree C and next incubated with 250ug of MEF lysate for 2 hours at 4 degree C.)
VPS35, Monoclonal Antibody (Cat# AAA27686)
Full Name
VPS35 Antibody, Clone 8A3: Biotin
Gene Names
VPS35; MEM3; PARK17
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
Purity
Protein G Purified
Pricing
IP (Immunoprecipitation)
(Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 5A9. Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts of (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degree C and next incubated with 250ug of MEF lysate for 2 hours at 4 degree C.)
VPS35, Monoclonal Antibody (Cat# AAA27675)
Full Name
VPS35 Antibody, Clone 5A9: ATTO 488
Gene Names
VPS35; MEM3; PARK17
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
Purity
Protein G Purified
Pricing
IP (Immunoprecipitation)
(Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 8A3. Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts of (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degree C and next incubated with 250ug of MEF lysate for 2 hours at 4 degree C.)
VPS35, Monoclonal Antibody (Cat# AAA27690)
Full Name
VPS35 Antibody, Clone 8A3: RPE
Gene Names
VPS35; MEM3; PARK17
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
Purity
Protein G Purified
Pricing
IP (Immunoprecipitation)
(Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 7E4. Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degree C and next incubated with 250ug of MEF lysate for 2 hours at 4 degree C.)
VPS35, Monoclonal Antibody (Cat# AAA27664)
Full Name
VPS35 Antibody, Clone 7E4
Gene Names
VPS35; MEM3; PARK17
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
Purity
Protein G Purified
Pricing
IP (Immunoprecipitation)
(Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 7E4. Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degree C and next incubated with 250ug of MEF lysate for 2 hours at 4 degree C.)
VPS35, Monoclonal Antibody (Cat# AAA27669)
Full Name
VPS35 Antibody, Clone 7E4: FITC
Gene Names
VPS35; MEM3; PARK17
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
Purity
Protein G Purified
Pricing
IP (Immunoprecipitation)
(Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 7E4. Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degree C and next incubated with 250ug of MEF lysate for 2 hours at 4 degree C.)
VPS35, Monoclonal Antibody (Cat# AAA27672)
Full Name
VPS35 Antibody, Clone 7E4: RPE
Gene Names
VPS35; MEM3; PARK17
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
Purity
Protein G Purified
Pricing
IP (Immunoprecipitation)
(Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 5A9. Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts of (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degree C and next incubated with 250ug of MEF lysate for 2 hours at 4 degree C.)
VPS35, Monoclonal Antibody (Cat# AAA27673)
Full Name
VPS35 Antibody, Clone 5A9
Gene Names
VPS35; MEM3; PARK17
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
Purity
Protein G Purified
Pricing
IP (Immunoprecipitation)
(Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 5A9. Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts of (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degree C and next incubated with 250ug of MEF lysate for 2 hours at 4 degree C.)
VPS35, Monoclonal Antibody (Cat# AAA27679)
Full Name
VPS35 Antibody, Clone 5A9: HRP
Gene Names
VPS35; MEM3; PARK17
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
Purity
Protein G Purified
Pricing
IP (Immunoprecipitation)
(Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 7E4. Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degree C and next incubated with 250ug of MEF lysate for 2 hours at 4 degree C.)
VPS35, Monoclonal Antibody (Cat# AAA27665)
Full Name
VPS35 Antibody, Clone 7E4: ATTO 390
Gene Names
VPS35; MEM3; PARK17
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
Purity
Protein G Purified
Pricing
IP (Immunoprecipitation)
(Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 7E4. Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degree C and next incubated with 250ug of MEF lysate for 2 hours at 4 degree C.)
VPS35, Monoclonal Antibody (Cat# AAA27666)
Full Name
VPS35 Antibody, Clone 7E4: ATTO 488
Gene Names
VPS35; MEM3; PARK17
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
Purity
Protein G Purified
Pricing
IP (Immunoprecipitation)
(Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 7E4. Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degree C and next incubated with 250ug of MEF lysate for 2 hours at 4 degree C.)
VPS35, Monoclonal Antibody (Cat# AAA27667)
Full Name
VPS35 Antibody, Clone 7E4: ATTO 594
Gene Names
VPS35; MEM3; PARK17
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
Purity
Protein G Purified
Pricing
IP (Immunoprecipitation)
(Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 7E4. Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degree C and next incubated with 250ug of MEF lysate for 2 hours at 4 degree C.)
VPS35, Monoclonal Antibody (Cat# AAA27668)
Full Name
VPS35 Antibody, Clone 7E4: Biotin
Gene Names
VPS35; MEM3; PARK17
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
Purity
Protein G Purified
Pricing