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471 results for " Goat Anti Human Whole Serum" - showing 1-50
ICC (Immunocytochemistry)
(Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-MARS mAb. [Lot No. MARSD10B4-2])
MARS, Monoclonal Antibody (Cat# AAA10600)
Full Name
Mouse monoclonal antibody Anti-Human MARS
Gene Names
MARS; MRS; METRS; MTRNS; SPG70
Reactivity
Human
Applications
Dot Blot, Immunocytochemistry, Immunoprecipitation, Western Blot, Flow Cytometry
Pricing
ICC (Immunocytochemistry)
(Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-STAT3 mAb. [Lot No. STAAD22A-1])
STAT3, Monoclonal Antibody (Cat# AAA10599)
Full Name
Mouse monoclonal antibody Anti-Human STAT3
Gene Names
STAT3; APRF; HIES; ADMIO
Reactivity
Human
Applications
Immunocytochemistry, Immunoprecipitation, Western Blot, Flow Cytometry
Pricing
ICC (Immunocytochemistry)
(Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-BTAF1 mAb. [Lot No. BTA3D61-1])
BTAF1, Monoclonal Antibody (Cat# AAA10597)
Full Name
Mouse monoclonal antibody Anti-Human BTAF1
Gene Names
BTAF1; MOT1; TAF172; TAFII170; TAF(II)170
Reactivity
Human
Applications
Dot Blot, Immunocytochemistry, Immunoprecipitation, Western Blot, Flow Cytometry
Pricing
Application Data
(At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
MAX, Polyclonal Antibody (Cat# AAA31386)
Full Name
Phospho-MAX (Ser11) Antibody
Gene Names
MAX; bHLHd4
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Zebrafish (83%), Bovine (100%), Horse (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (100%)
Predicted Reactivity: Pig (100%), Zebrafish (83%), Bovine (100%), Horse (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (100%)
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
Application Data
(At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody(Red), diluted at 1/600, was used as secondary antibody.)
HSL, Polyclonal Antibody (Cat# AAA31384)
Full Name
Phospho-HSL (Ser660) Antibody
Gene Names
LIPE; HSL; LHS
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (88%), Horse (89%), Dog (100%)
Predicted Reactivity: Pig (100%), Bovine (88%), Horse (89%), Dog (100%)
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
Application Data
(At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
Nucleophosmin, Polyclonal Antibody (Cat# AAA31463)
Full Name
Phospho-Nucleophosmin (Thr237) Antibody
Gene Names
NPM1; B23; NPM
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (89%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%)
Predicted Reactivity: Pig (100%), Bovine (89%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%)
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
Application Data
(At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
RBL2, Polyclonal Antibody (Cat# AAA31451)
Full Name
Phospho-RBL2 (Thr642) Antibody
Gene Names
RBL2; Rb2; P130
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (89%), Sheep (100%), Rabbit (89%), Dog (100%)
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (89%), Sheep (100%), Rabbit (89%), Dog (100%)
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IF (Immunofluorescence)
(Immunofluorescence staining of Raw264.7 cells with AAA27029 at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).)
CD14, Monoclonal Antibody (Cat# AAA27029)
Full Name
CD14 Monoclonal Antibody
Reactivity
Human, Mouse, Rat, Rabbit
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
>95%, Protein A Purified
Pricing
IHC (Immunohistchemistry)
(AAA31095 at 1/100 staining Human kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Histone H3, Polyclonal Antibody (Cat# AAA31095)
Full Name
Histone H3 Antibody
Gene Names
HIST1H3A; H3/A; H3FA
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
FCM (Flow Cytometry)
(Figure 10. Flow Cytometry analysis of THP-1 cells using anti-HDAC5 antibody (AAA19236).Overlay histogram showing THP-1 cells stained with AAA19236 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HDAC5 Antibody (AAA19236, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
HDAC5, Polyclonal Antibody (Cat# AAA19236)
Full Name
Anti-HDAC5 Antibody
Gene Names
HDAC5; HD5; NY-CO-9
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Direct ELISA
Purity
Immunogen affinity purified.
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of HepG2 cells using AAA26922 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
14-3-3 protein beta/alpha, Polyclonal Antibody (Cat# AAA26922)
Full Name
Rabbit anti-human 14-3-3 protein beta/alpha polyclonal Antibody
Gene Names
YWHAB; HS1; GW128; YWHAA; KCIP-1; HEL-S-1
Reactivity
Human, mouse, rat
Applications
Western Blot, Immunohistochemistry
Purity
Caprylic Acid Ammonium Sulfate Precipitation purified
Pricing
IP (Immunoprecipitation)
(Figure 11 Immunoprecipitation Validation in HEK293 cells (Kawai et al., 2004)HEK293 cells were transiently transfected with DYKDDDDK-IRF7. Ccell lysates were immunoprecipitated with control rabbit anti-mouse immunoglobulin serum (IgG) or anti-MyD88 (Ab1 and Ab2), followed by immunoblotting with anti-DYKDDDDK.)
MYD88, Polyclonal Antibody (Cat# AAA10944)
Full Name
MYD88 Antibody
Gene Names
MYD88; MYD88D
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
MYD88 Antibody is affinity chromatography purified via peptide column.
Pricing
IHC (Immunohistchemistry)
(At 1/200 staining Human kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)
BRCA1, Polyclonal Antibody (Cat# AAA31415)
Full Name
Phospho-BRCA1 (Ser1497) Antibody
Gene Names
BRCA1; IRIS; PSCP; BRCAI; BRCC1; PNCA4; RNF53; BROVCA1; PPP1R53
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Bovine (80%), Dog (80%)
Predicted Reactivity: Bovine (80%), Dog (80%)
Applications
Western Blot, Immunohistochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
WB (Western Blot)
(Western blot analysis of HDAC5 expression in mouse brain tissue lysates, The lane on the right is treated with the antigen-specific peptide.)
HDAC5, Polyclonal Antibody (Cat# AAA31097)
Full Name
HDAC5 Antibody
Gene Names
HDAC5; HD5; NY-CO-9
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
FCM (Flow Cytometry)
(Overlay histogram showing Hela cells stained with CSB-MA878942A1m (red line) at 1:300. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4 degree C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4 degree C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.)
PD-L1, Monoclonal Antibody (Cat# AAA27017)
Full Name
PD-L1 Monoclonal Antibody
Gene Names
CD274; B7-H; B7H1; PDL1; PD-L1; PDCD1L1; PDCD1LG1
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry
Purity
>95%
Protein G Purified
Protein G Purified
Pricing
Application Data
(Figure 10 KD Validation in HeLa cells (Horinaka et al., 2005)HeLa cells were transfected with DR4siRNA or LacZ control siRNA. At 24 h after transfection, the cells were treated with or without 20 ?M luteolin for 24 h. Western blot analysis was carried out with anti-DR4 antibodies (1139). DR4 expression was markedly reduced after DR4 knockdown.)
DR4, Polyclonal Antibody (Cat# AAA10952)
Full Name
DR4 Antibody
Gene Names
TNFRSF10A; DR4; APO2; CD261; TRAILR1; TRAILR-1
Reactivity
Human
Applications
Western Blot, Immunocytochemistry
Purity
DR4 Antibody is Antibody is affinity chromatography purified via peptide column.
Pricing
IF (Immunofluorescence)
(AAA31089 staining 293 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
AKT, Polyclonal Antibody (Cat# AAA31089)
Full Name
AKT Antibody
Gene Names
AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Immunoprecipitation
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
Application Data
(At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
FOXO4, Polyclonal Antibody (Cat# AAA31400)
Full Name
Phospho-FOXO4 (Thr455) Antibody
Gene Names
FOXO4; AFX; AFX1; MLLT7
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Bovine (91%), Horse (83%), Sheep (91%), Dog (91%)
Predicted Reactivity: Bovine (91%), Horse (83%), Sheep (91%), Dog (91%)
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistochemistry)
(At 1/200 staining Human lung cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)
Caveolin 2, Polyclonal Antibody (Cat# AAA31397)
Full Name
Phospho-Caveolin 2 (Ser36) Antibody
Gene Names
CAV2; CAV
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
Application Data
(At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
EGFR, Polyclonal Antibody (Cat# AAA31399)
Full Name
Phospho-EGFR (Tyr998) Antibody
Gene Names
EGFR; ERBB; HER1; mENA; ERBB1; PIG61
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Sheep (100%), Rabbit (100%)
Predicted Reactivity: Pig (100%), Bovine (100%), Sheep (100%), Rabbit (100%)
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
Application Data
(At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
KIF1C, Polyclonal Antibody (Cat# AAA31396)
Full Name
Phospho-KIF1C (Ser1092) Antibody
Gene Names
KIF1C; SAX2; LTXS1; SATX2; SPAX2; SPG58
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%)
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%)
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
WB (Western Blot)
(Western blot analysis of extracts of various sample, using Catenin-beta antibody.)
Catenin-beta, Polyclonal Antibody (Cat# AAA31090)
Full Name
Catenin-beta Antibody
Gene Names
CTNNB1; EVR7; CTNNB; MRD19; armadillo
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
Application Data
(At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
p70 S6 Kinase, Polyclonal Antibody (Cat# AAA31408)
Full Name
Phospho-p70 S6 Kinase (Ser427) Antibody
Gene Names
RPS6KB1; S6K; PS6K; S6K1; STK14A; p70-S6K; p70 S6KA; p70-alpha; S6K-beta-1; p70(S6K)-alpha
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (100%)
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (100%)
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
Application Data
(At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
AXL, Polyclonal Antibody (Cat# AAA31469)
Full Name
Phospho-AXL (Tyr702) Antibody
Gene Names
AXL; UFO; JTK11
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Zebrafish (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Xenopus (100%)
Predicted Reactivity: Pig (100%), Zebrafish (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Xenopus (100%)
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
Application Data
(At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody(Red), diluted at 1/600, was used as secondary antibody.)
ATP1A1, Polyclonal Antibody (Cat# AAA31454)
Full Name
Phospho-ATP1A1 (Tyr260) Antibody
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (90%), Dog (100%), Xenopus (90%)
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (90%), Dog (100%), Xenopus (90%)
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
FCM (Flow Cytometry)
(Figure 7. Flow Cytometry analysis of U251 cells using anti-CGKI/PRKG1 antibody (AAA19246).Overlay histogram showing U251 cells stained with AAA19246 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CGKI/PRKG1 Antibody (AAA19246, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
cGKI/PRKG1, Polyclonal Antibody (Cat# AAA19246)
Full Name
Anti-cGKI/PRKG1 Antibody
Gene Names
PRKG1; 1; PKG; cGK; AAT8; cGK1; cGKI; cGK 1; PRKG1B; PRKGR1B; cGKI-BETA; cGKI-alpha
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Direct ELISA
Purity
Immunogen affinity purified.
Pricing
Application Data
(At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Smad2, Polyclonal Antibody (Cat# AAA31390)
Full Name
Phospho-Smad2 (Ser255) Antibody
Gene Names
SMAD2; JV18; MADH2; MADR2; JV18-1; hMAD-2; hSMAD2
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Zebrafish (89%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Xenopus (89%)
Predicted Reactivity: Zebrafish (89%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Xenopus (89%)
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistchemistry)
(AAA31057 at 1/200 staining human colon tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Acetyl-Histone H3, Polyclonal Antibody (Cat# AAA31057)
Full Name
Acetyl-Histone H3 (Lys9) Antibody
Gene Names
HIST1H3A; H3/A; H3FA
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
Immunogen affinity purified
Pricing
FCM (Flow Cytometry)
(Overlay histogram showing HepG2 cells stained with AAA28061 (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 1 h at 4 degree C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4 degree C. Isotype control antibody (green line) was mouse IgG2b (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
CD63, Monoclonal Antibody (Cat# AAA28061)
Full Name
CD63 Monoclonal Antibody
Gene Names
Cd63; ME491; C75951; Tspan30
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry
Purity
>95%, Protein A purified
Pricing
IHC (Immunohistochemistry)
(AAA31085 at 1/200 staining Human kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
C-Kit, Polyclonal Antibody (Cat# AAA31085)
Full Name
C-Kit Antibody
Gene Names
KIT; PBT; SCFR; C-Kit; CD117; MASTC
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
Application Data
(At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody(Red), diluted at 1/600, was used as secondary antibody.)
PKC theta, Polyclonal Antibody (Cat# AAA31410)
Full Name
Phospho-PKC theta (Tyr90) Antibody
Gene Names
PRKCQ; PRKCT; nPKC-theta
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (91%), Dog (91%)
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (91%), Dog (91%)
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
Application Data
(At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
FGFR2, Polyclonal Antibody (Cat# AAA31452)
Full Name
Phospho-FGFR2 (Ser782) Antibody
Gene Names
FGFR2; BEK; JWS; BBDS; CEK3; CFD1; ECT1; KGFR; TK14; TK25; BFR-1; CD332; K-SAM
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Zebrafish (88%), Bovine (100%), Horse (100%), Rabbit (83%), Dog (100%), Chicken (100%), Xenopus (88%)
Predicted Reactivity: Zebrafish (88%), Bovine (100%), Horse (100%), Rabbit (83%), Dog (100%), Chicken (100%), Xenopus (88%)
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
Application Data
(Immunoperoxidase staining of human tonsil cryosection using Mouse anti Human CD3 antibody, clone UCHT1 followed by horseradish peroxidase Goat anti Mouse IgG2a antibody as a detection reagent. Medium power)
CD3, Monoclonal Antibody (Cat# AAA11877)
Full Name
MOUSE ANTI HUMAN CD3:FITC
Gene Names
CD3G; T3G; IMD17; CD3-GAMMA
Applications
Flow Cytometry
Pricing
Application Data
(At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
HSF1, Polyclonal Antibody (Cat# AAA31391)
Full Name
Phospho-HSF1 (Ser307) Antibody
Gene Names
HSF1; HSTF1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
Application Data
(At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
Smad2, Polyclonal Antibody (Cat# AAA31433)
Full Name
Phospho-Smad2 (Ser465+Ser467) Antibody
Gene Names
SMAD2; JV18; MADH2; MADR2; JV18-1; hMAD-2; hSMAD2
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Zebrafish (100%), Bovine (100%), Sheep (100%), Rabbit (100%), Dog (100%), Xenopus (100%)
Predicted Reactivity: Pig (100%), Zebrafish (100%), Bovine (100%), Sheep (100%), Rabbit (100%), Dog (100%), Xenopus (100%)
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
WB (Western Blot)
(Western blot analysis of extracts from various samples, using Phospho-ICAM-1 (Tyr512) Antibody. Lane 1: VERO cells, treated with blocking peptideLane 2: VERO cellsLane 3: A2780 cells.)
ICAM-1, Polyclonal Antibody (Cat# AAA30979)
Full Name
Phospho-ICAM-1 (Tyr512) Antibody
Gene Names
ICAM1; BB2; CD54; P3.58
Reactivity
Human, Mouse, Monkey
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
Peptide affinity purification
Pricing
FCM (Flow Cytometry)
(Figure 6. Flow Cytometry analysis of HEPG2 cells using anti-Alkaline phosphatase/ALPL antibody (AAA19231).Overlay histogram showing HEPG2 cells stained with AAA19231 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Alkaline phosphatase/ALPL Antibody (AAA19231, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Alkaline phosphatase/ALPL, Polyclonal Antibody (Cat# AAA19231)
Full Name
Anti-Alkaline phosphatase/ALPL Antibody
Gene Names
ALPL; HOPS; TNAP; APTNAP; TNSALP; AP-TNAP
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistochemistry)
(Figure 8. IHC analysis of MEK2/MAP2K2 using anti-MEK2/MAP2K2 antibody (AAA19230).MEK2/MAP2K2 was detected in paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-MEK2/MAP2K2 Antibody (AAA19230) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)
MEK2/MAP2K2, Polyclonal Antibody (Cat# AAA19230)
Full Name
Anti-MEK2/MAP2K2 Antibody
Gene Names
MAP2K2; CFC4; MEK2; MKK2; MAPKK2; PRKMK2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Direct ELISA
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(Figure 1. Western blot analysis of Lyn using anti-Lyn antibody (AAA19150). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human 293T whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Lyn antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Lyn at approximately 59KD. The expected band size for Lyn is at 59KD.)
Lyn, Polyclonal Antibody (Cat# AAA19150)
Full Name
Anti-Lyn Picoband Antibody
Gene Names
LYN; JTK8; p53Lyn; p56Lyn
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
IHC, WB
Purity
Immunogen affinity purified
Pricing
FCM (Flow Cytometry)
(Figure 8. Flow Cytometry analysis of A549 cells using anti-MCM4 antibody (AAA19257).Overlay histogram showing A549 cells stained with AAA19257 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MCM4 Antibody (AAA19257, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
MCM4, Polyclonal Antibody (Cat# AAA19257)
Full Name
Anti-MCM4 Antibody
Gene Names
MCM4; NKCD; CDC21; CDC54; NKGCD; hCdc21; P1-CDC21
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Direct ELISA
Purity
Immunogen affinity purified.
Pricing
IP (Immunoprecipitation)
(MYEF2 was immunoprecipitated using: Lane A:0.5 mg SH-SY5Y Whole Cell Lysate 4 uL anti-MYEF2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-MYEF2 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 64 kDa Observed band size :64 kDa)
MYEF2, Polyclonal Antibody (Cat# AAA27756)
Full Name
Anti-MYEF2 Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
Purity
Protein A & Antigen Affinity
Pricing
FCM (Flow Cytometry)
(Figure 8. Flow Cytometry analysis of A549 cells using anti-Caveolin-2/CAV2 antibody (AAA19243).Overlay histogram showing A549 cells stained with AAA19243 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Caveolin-2/CAV2 Antibody (AAA19243,1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Caveolin-2/CAV2, Polyclonal Antibody (Cat# AAA19243)
Full Name
Anti-Caveolin-2/CAV2 Antibody
Gene Names
CAV2; CAV
Reactivity
Human, Mouse, Rat, Monkey
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Direct ELISA
Purity
Immunogen affinity purified.
Pricing
ICC (Immunocytochemistry)
(Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-TWIST mAb. [Lot No. Twist2C1a-1])
TWIST, Monoclonal Antibody (Cat# AAA10606)
Full Name
Mouse monoclonal antibody Anti-Human TWIST
Gene Names
TWIST1; CRS; CSO; SCS; ACS3; CRS1; BPES2; BPES3; TWIST; bHLHa38
Reactivity
Human
Applications
Dot Blot, Immunocytochemistry, Western Blot, Flow Cytometry
Pricing
ICC (Immunocytochemistry)
(Figure 10 Immunocytochemistry Validation of PD-L1Immunocytochemical analysis of 4% paraformaldehyde-fixed PD-L1 transfected 293 cells labeling PD-L1 with at 1 μg/ml, followed by Goat anti-mouse IgG secondary antibody at 1/250 dilution (red). Lower left: Use mouse IgG antibody at 1 μg/ml as control.)
PDL1, Monoclonal Antibody (Cat# AAA10992)
Full Name
PDL1 Antibody [6H10]
Gene Names
CD274; B7-H; B7H1; PDL1; PD-L1; PDCD1L1; PDCD1LG1
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence
Purity
Protein A purified
Pricing
FCM (Flow Cytometry)
(Figure 8. Flow Cytometry analysis of C6 cells using anti-non-muscle Myosin IIB/MYH10 antibody (AAA19262).Overlay histogram showing C6 cells stained with AAA19262 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-non-muscle Myosin IIB/MYH10 Antibody (AAA19262,1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
non-muscle Myosin IIB/MYH10, Polyclonal Antibody (Cat# AAA19262)
Full Name
Anti-non-muscle Myosin IIB/MYH10 Antibody
Gene Names
MYH10; NMMHCB; NMMHC-IIB
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistchemistry)
(At 1/200 staining Human breast tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)
KAT3B/p300, Polyclonal Antibody (Cat# AAA31393)
Full Name
Phospho-KAT3B/p300 (Ser1834) Antibody
Gene Names
EP300; p300; KAT3B; RSTS2
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Chicken (100%), Xenopus (100%)
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Chicken (100%), Xenopus (100%)
Applications
Western Blot, Immunohistochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistchemistry)
(AAA31092 at 1/200 staining human breast cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
STAT3, Polyclonal Antibody (Cat# AAA31092)
Full Name
STAT3 Antibody
Gene Names
STAT3; APRF; HIES; ADMIO; ADMIO1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
WB (Western Blot)
(Western blot analysis of p70 S6 Kinase expression in mouse muscle tissue lysates, The lane on the right is treated with the antigen-specific peptide.)
p70 S6 Kinase, Polyclonal Antibody (Cat# AAA31088)
Full Name
p70 S6 Kinase Antibody
Gene Names
RPS6KB1; S6K; PS6K; S6K1; STK14A; p70-S6K; p70 S6KA; p70-alpha; S6K-beta-1; p70(S6K)-alpha
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
FCM (Flow Cytometry)
(Figure 6. Flow Cytometry analysis of U-87 cells using anti-ATP citrate lyase antibody (AAA11689).Overlay histogram showing U-87 cells stained with AAA11689 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ATP citrate lyase Antibody (AAA11689,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
ATP citrate lyase, Polyclonal Antibody (Cat# AAA11689)
Full Name
Anti-ATP citrate lyase Antibody
Gene Names
ACLY; ACL; ATPCL; CLATP
Reactivity
Human, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen affinity purified.
Pricing
Application Data
(At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Tuberin/TSC2, Polyclonal Antibody (Cat# AAA31441)
Full Name
Phospho-Tuberin/TSC2 (Ser1254) Antibody
Gene Names
TSC2; LAM; TSC4
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%)
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%)
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing