Search results for " Single pass type I membrane protein"

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WB (Western Blot) (Western blot analysis of RYK (arrow) using rabbit polyclonal RYK Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the RYK gene (Lane 2) (Origene Technologies).)

RYK, Polyclonal Antibody (Cat# AAA28767)

Full Name

RYK Antibody

Gene Names

RYK; JTK5; RYK1; JTK5A; D3S3195

Reactivity

Human, mouse, rat

Applications

Western Blot, Immunohistochemistry

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

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IHC (Immunohistchemistry) (At 1/100 staining Human colorectal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

E-cadherin, Polyclonal Antibody (Cat# AAA31444)

Full Name

Phospho-E-cadherin (Ser844) Antibody

Gene Names

CDH1; UVO; CDHE; ECAD; LCAM; Arc-1; CD324

Reactivity

Human, Mouse, Rat, Monkey
Predicted Reactivity: Pig (100%), Bovine (91%), Horse (91%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (83%), Xenopus (100%)

Applications

Western Blot, Immunohistochemistry, Peptide ELISA

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

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Application Data (At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

EGFR, Polyclonal Antibody (Cat# AAA31399)

Full Name

Phospho-EGFR (Tyr998) Antibody

Gene Names

EGFR; ERBB; HER1; mENA; ERBB1; PIG61

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Sheep (100%), Rabbit (100%)

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

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Application Data (At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

AXL, Polyclonal Antibody (Cat# AAA31469)

Full Name

Phospho-AXL (Tyr702) Antibody

Gene Names

AXL; UFO; JTK11

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Zebrafish (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Xenopus (100%)

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

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Application Data (At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)

FGFR2, Polyclonal Antibody (Cat# AAA31452)

Full Name

Phospho-FGFR2 (Ser782) Antibody

Gene Names

FGFR2; BEK; JWS; BBDS; CEK3; CFD1; ECT1; KGFR; TK14; TK25; BFR-1; CD332; K-SAM

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Zebrafish (88%), Bovine (100%), Horse (100%), Rabbit (83%), Dog (100%), Chicken (100%), Xenopus (88%)

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

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WB (Western Blot) (Western blot analysis of lysates from Hela, HepG2, MCF-7, SH-SY5Y, mouse NIH/3T3 cell line (from left to right), using FGFR1 Antibody (Center). AAA28687 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)

FGFR1, Polyclonal Antibody (Cat# AAA28687)

Full Name

FGFR1 Antibody (Center)

Gene Names

FGFR1; CEK; FLG; HH2; OGD; FLT2; KAL2; BFGFR; CD331; FGFBR; FLT-2; HBGFR; N-SAM; FGFR-1; HRTFDS; bFGF-R-1

Reactivity

Human, mouse (Predicted Reactivity: Chicken, Rat)

Applications

Flow Cytometry, Western Blot

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

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Integrin Beta 3 (ITGb3), Recombinant Protein (Cat# AAA20259)

Full Name

Recombinant Integrin Beta 3 (ITGb3)

Gene Names

ITGB3; GT; CD61; GP3A; BDPLT2; GPIIIa; BDPLT16

Reactivity

Human

Applications

Western Blot, Immunoprecipitation, Chromatin Immunoprecipitation

Purity

>98%

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 cells using HLA-DPB1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

HLA-DPB1, Polyclonal Antibody (Cat# AAA28352)

Full Name

HLA-DPB1 Rabbit pAb

Gene Names

HLA-DPB1; DPB1; HLA-DP; HLA-DPB; HLA-DP1B

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity purification

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using APP antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

APP, Polyclonal Antibody (Cat# AAA28326)

Full Name

APP Rabbit pAb

Gene Names

APP; AAA; AD1; PN2; ABPP; APPI; CVAP; ABETA; PN-II; CTFgamma

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity purification

Pricing

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Application Data (At 37 degree C. The primary antibody was diluted 1/200 and incubated with the sample for 1 hour at 37 degree C. A Alexa Fluor 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.)

N Cadherin, Polyclonal Antibody (Cat# AAA31337)

Full Name

N Cadherin Antibody

Gene Names

CDH2; CDHN; NCAD; CD325; CDw325

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Zebrafish (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (100%)

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA, Immunohistochemistry, Immunohistochemistry

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin

Pricing

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Application Data (At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

CD22, Polyclonal Antibody (Cat# AAA31418)

Full Name

Phospho-CD22 (Tyr842) Antibody

Gene Names

CD22; SIGLEC2; SIGLEC-2

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Horse (83%)

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

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IHC (Immunohistchemistry) (At 1/200 staining Human kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)

FGFR1/2/3/4, Polyclonal Antibody (Cat# AAA31417)

Full Name

Phospho-FGFR1/2/3/4 (Tyr653+Tyr654) Antibody

Gene Names

FGFR1; CEK; FLG; HH2; OGD; FLT2; KAL2; BFGFR; CD331; FGFBR; FLT-2; HBGFR; N-SAM; FGFR-1; bFGF-R-1

Reactivity

Human, Mouse, Rat, Monkey
Predicted Reactivity: Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (100%)

Applications

Western Blot, Immunohistochemistry, Peptide ELISA

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

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Interferon Gamma Receptor 1 (IFNgR1), Recombinant Protein (Cat# AAA20238)

Full Name

Recombinant Interferon Gamma Receptor 1 (IFNgR1)

Gene Names

Ifngr1; Ifgr; CD119; Ifngr; Nktar; IFN-gammaR

Reactivity

Mouse

Applications

Western Blot, Immunoprecipitation, Chromatin Immunoprecipitation

Purity

> 95%

Pricing

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Application Data (At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody(Red), diluted at 1/600, was used as secondary antibody.)

Ret, Polyclonal Antibody (Cat# AAA31389)

Full Name

Phospho-Ret (Tyr1096) Antibody

Gene Names

RET; PTC; MTC1; HSCR1; MEN2A; MEN2B; RET51; CDHF12; CDHR16; RET-ELE1

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Sheep (100%), Rabbit (100%), Dog (100%)

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

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IHC (Immunohistochemistry) (Formalin-fixed and paraffin-embedded human cerebellum reacted with GARP Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

GARP, Polyclonal Antibody (Cat# AAA28686)

Full Name

GARP Antibody (Center)

Gene Names

LRRC32; GARP; D11S833E

Reactivity

Human, mouse

Applications

Western Blot, Immunohistochemistry

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

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CD227/MUC1, Polyclonal Antibody (Cat# AAA31426)

Full Name

Phospho-CD227/MUC1 (Ser1227) Antibody

Gene Names

MUC1; EMA; PEM; PUM; KL-6; MAM6; PEMT; CD227; H23AG; MUC-1; CA 15-3; MUC-1/X; MUC1/ZD; MUC-1/SEC

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Peptide ELISA

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

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IRE1, Polyclonal Antibody (Cat# AAA28358)

Full Name

IRE1 Rabbit pAb

Gene Names

ERN1; IRE1; IRE1P; IRE1a; hIRE1p

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity purification

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of NIH-3T3 cells using IFNAR1 Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

IFNAR1, Polyclonal Antibody (Cat# AAA28371)

Full Name

IFNAR1 Rabbit pAb

Gene Names

IFNAR1; AVP; IFRC; IFNAR; IFNBR; IFN-alpha-REC

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF

Purity

Affinity purification

Pricing

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IHC (Immunohistchemistry) (Figure 6. IHC analysis of Integrin alpha 5 using anti-Integrin alpha 5 antibody (AAA19156).Integrin alpha 5 was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Integrin alpha 5 Antibody (AAA19156) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

Integrin alpha 5, Polyclonal Antibody (Cat# AAA19156)

Full Name

Anti-Integrin alpha 5 Picoband antibody

Gene Names

ITGA5; FNRA; CD49e; VLA-5; VLA5A

Reactivity

Human, Mouse, Rat
No cross reactivity with other proteins.

Applications

EIA, IHC, WB

Pricing

Coronavirus OC43 Spike Glycoprotein (S), Recombinant Protein (Cat# AAA27034)

Full Name

Recombinant Human coronavirus OC43 Spike glycoprotein (S), partial

Purity

Greater or equal to 85% purity as determined by SDS-PAGE.

Pricing

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Application Data (Analysis of Protein Array containing >19, 000 full-length human proteins using TACSTD2 Mouse Monoclonal Antibody (TACSTD2/2153) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)

TACSTD2/TROP2, Monoclonal Antibody (Cat# AAA23900)

Full Name

TACSTD2/TROP2 (Epithelial Marker)

Gene Names

TACSTD2; EGP1; GP50; M1S1; EGP-1; TROP2; GA7331; GA733-1

Reactivity

Human. Others not known.

Applications

Immunohistochemistry

Pricing

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IHC (Immunohistochemistry) (Figure 7. IHC analysis of CD105 using anti-CD105 antibody (AAA19165).CD105 was detected in paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CD105 Antibody (AAA19165) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

CD105, Polyclonal Antibody (Cat# AAA19165)

Full Name

Anti-CD105 Picoband Antibody

Gene Names

Eng; Endo; CD105; AI528660; AI662476; S-endoglin

Reactivity

Mouse, Rat
No cross reactivity with other proteins.

Applications

EIA, IHC, WB

Purity

Immunogen affinity purified

Pricing

PVRL4, Polyclonal Antibody (Cat# AAA28678)

Full Name

PVRL4 Antibody (Center)

Gene Names

PVRL4; LNIR; PRR4; EDSS1; nectin-4

Reactivity

Human

Applications

Western Blot, Dot Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, Flow Cytometry

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

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FCM (Flow Cytometry) (Staining of thrombin activated platelets with Mouse anti Human CD62P)

CD62P, Monoclonal Antibody (Cat# AAA12247)

Full Name

MOUSE ANTI HUMAN CD62P

Gene Names

SELP; CD62; GRMP; PSEL; CD62P; GMP140; LECAM3; PADGEM

Applications

Immunohistochemistry, Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot

Pricing

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IHC (Immunohistchemistry) (Formalin-fixed and paraffin-embedded human lymph reacted with CD19 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

CD19, Polyclonal Antibody (Cat# AAA28685)

Full Name

CD19 Antibody (N-term)

Gene Names

CD19; B4; CVID3

Reactivity

Human

Applications

Western Blot, Immunofluorescence, Flow Cytometry, Immunohistochemistry

Purity

This antibody is purified through a protein A column, followed by peptide affinity purification.

Pricing

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FCM (Flow Cytometry) (MUSK Antibody (AAA28697) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

MUSK, Polyclonal Antibody (Cat# AAA28697)

Full Name

MUSK Antibody

Gene Names

MUSK; CMS9

Reactivity

Human, mouse

Applications

Western Blot, Flow Cytometry, Immunofluorescence

Purity

Purified Rabbit Polyclonal Antibody (PAB)

Pricing

ERN2, Polyclonal Antibody (Cat# AAA28749)

Full Name

ERN2 Antibody (N-term)

Gene Names

ERN2; IRE1b; IRE2p; hIRE2p; IRE1-BETA

Reactivity

Human

Applications

Western Blot

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

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FCM (Flow Cytometry) (WB - MCSF Receptor (CSF1R) Antibody (C-term) AAA28757 detail IHC-P - MCSF Receptor (CSF1R) Antibody (C-term) AAA28757 detail IHC-P - MCSF Receptor (CSF1R) Antibody (C-term) AAA28757 detail FC - MCSF Receptor (CSF1R) Antibody (C-term) AAA28757 detail Overlay histogram showing HepG2 cells stained with AAA28757(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AAA28757, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(1583138) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)

MCSF Receptor (CSF1R), Polyclonal Antibody (Cat# AAA28757)

Full Name

MCSF Receptor (CSF1R) Antibody (C-term)

Gene Names

CSF1R; FMS; CSFR; FIM2; HDLS; C-FMS; CD115; CSF-1R; M-CSF-R

Reactivity

Human

Applications

Western Blot, Flow Cytometry, Immunohistochemistry

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

TIGIT, Polyclonal Antibody (Cat# AAA28803)

Full Name

TIGIT Antibody

Gene Names

TIGIT; VSIG9; VSTM3; WUCAM

Reactivity

Human, Mouse

Applications

Western Blot

Purity

This antibody is purified through a protein A column, followed by peptide affinity purification.

Pricing

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IHC (Immunohistchemistry) (At 1/100 staining Mouse heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

Ephrin B1/B2/B3, Polyclonal Antibody (Cat# AAA31292)

Full Name

Phospho-Ephrin B1/B2/B3 (Tyr324) Antibody

Gene Names

EFNB1; CFND; CFNS; EFL3; EPLG2; Elk-L; LERK2

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Peptide ELISA

Purity

Peptide affinity purification

Pricing

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WB (Western Blot) (HHLA2 Antibody (N-term) western blot analysis in MDA-MB453,CEM,HL-60,MDA-MB231 cell line lysates (35ug/lane).This demonstrates the HHLA2 antibody detected the HHLA2 protein (arrow).)