At AAA Biotech, we offer a comprehensive selection of high-quality recombinant proteins for use in a wide range of research areas, including immunology, neuroscience, stem cell research, cancer research and more. No matter whether you need recombinant proteins for cell expansion, polarization, differentiation, or cell processing applications, we have got you covered.
Our recombinant proteins undergo rigorous quality testing. So, you can rely on AAA Biotech for high-quality recombinant proteins to support your research. Explore our catalog to find the right protein for your research needs.
Application Data (Coomassiegelstainofhumandopamine-stabilizedalphasynucleinoligomers(AAA27710).Load:2ug.)
Application Data (Evaluationofa-syntoxicityonprimarymousecorticalneurons.Mitochondrialdehydrogenaseactivity,whichreducesyellowMTTtodarkblueformazancrystals,isareactionthatiscatalyzedinlivingcells.CellviabilitywasassessedwithanMTTassayanddisplayedas%ofvehiclecontrol(VC).Dataarepresentedasbargraphsandstandarddeviation.ForstatisticalanalysisOne-wayANOVAfollowedbyBonferronipost-hoctest(vsVC)wasused.**p)
Application Data (Evaluationofa-syntoxicityonprimarymousecorticalneurons.Lactatedehydrogenase(LDH)isasolubleenzymepresentinthecytosolthatisreleaseduponcelldeath.ToxicitywasassessedwithanLDHassayanddisplayedas%ofvehiclecontrol(VC).Dataarepresentedasbargraphsandstandarddeviation.ForstatisticalanalysisOne-wayANOVAfollowedbyBonferronipost-hoctest(vsVC)wasused.***p)
Application Data (HPLCofAlphaSynucleinProteinOligomers(AAA27710)randownasizeexclusioncolumn.Wavelength:280nm.)
Application Data (TEMofHumanRecombinantAlphaSynucleinOligomers(DopamineHCLStabilized)(AAA27710))
Application Data (TEMofHumanRecombinantAlphaSynucleinOligomers(DopamineHCLStabilized)(AAA27710))
SDS-PAGE (Purity Control in SDS-PAGE: 15%SDS-PAGE analysis (15%) of 10 ul of recombinant allergen. Purity is >95% as determined by gel electrophoresis.)
ELISA (Protein concentration determined espectrophotometricallyThis protein does not contain any Trp residues. Experience shows that this could result in more than 10% error in the computed extinction coefficient. Therefore, we have measured the protein concentration by using the colorimetric assay based on the interaction between Coomassie brilliant blue and the arginine and aromatic residues (Bradford Method) and its maximum absorption shifts from 470 nm to 595 nm. The standard curve was performed with the protein BSA.)
WB (Western Blot) (Western Blot analysis of 10 ng Lin28a protein (Prod. No. AAA14519) probed withLane 1: polyclonal anti-Lin28 antibodyLane 2: anti-6x His tag antibody.)
SDS-PAGE (SDS-PAGE Analysis: Lane 1: MW marker; Lane 2: 1.0 ?g of purified Lin28a protein)
ELISA (ELISA plate was coated by N protein, 100 uL/cell, at various concentrations. The direct ELISA analysis was performed by loading 100 uL per well of a anti C-ter His tag HRP conjugated mAb at a concentration of 1:2000. The plate was incubated for 1 hours at 37 degree C, then washed 5 time. Detection was performed using TMB substrate for 10 minutes at room temperature in the dark. The plate was stopped with 2M sulfuric acid. Absorbances were read on a spectrophotometer at 450 nm.)
Chemiluminescence Immunoassay, Lateral Flow, Western Blot
Purity
> 90% pure by SDS-PAGE Ligand affinity chromatography
Pricing
What Are Recombinant Proteins?
Recombinant proteins are purified laboratory reagents produced through genetic engineering. A specific gene of interest is inserted into a host organism, such as mammalian, bacterial, yeast, or insect cells, which then expresses the protein in a controlled environment.
The recombinant process utilized to generate the recombinant proteins in our catalog provides precise control over sequence modifications, expression levels, and large-scale production tailored to experimental needs. These recombinants are widely used in research to investigate protein-protein interactions, enzyme activities, receptor-ligand binding, and cellular responses. Additionally, recombinant proteins serve as standards or controls in immunostaining assays and support cell growth and differentiation in culture systems, particularly in immunology, oncology, and structural biology studies.
Common Applications of Recombinant Proteins
Studying protein-protein and protein-DNA interactions
Functional assays to study biological pathways
Standard curves in ELISA and other quantitative assays
Use as antigens for antibody production
Development and screening of therapeutic drugs
Biomarker discovery and validation
Cell signaling and immune response studies
Vaccine research and development
Key Features of AAA Biotech’s Recombinant Proteins
High Purity: Most proteins are purified to ≥95% using affinity chromatography and other validated techniques.
Biological Activity: If functional activity is tested and confirmed for a given protein, it will be noted directly on the product page.
Multiple Expression Systems: Available in E. coli, HEK293, CHO, yeast, and insect cells to match your assay requirements.
Custom Tags Available: His-tag, GST, FLAG, and Fc fusion options for easy purification and detection.
Wide Range of Targets: Cytokines, growth factors, enzymes, receptors, signaling proteins, and more.
Validated Consistency: Lot-to-lot consistency ensured through rigorous QC protocols.
Flexible Quantities: Available in various pack sizes to suit different experimental needs.
Ready-to-Use Format: Lyophilized or liquid formulations that are easy to reconstitute and use.
Why Buy Recombinant Proteins from AAA Biotech?
At AAA Biotech, we are committed to supporting the research community with recombinant proteins that offer exceptional performance and reliability. Our proteins are produced using industry-standard methods and are validated to meet the needs of academic, pharmaceutical, and biotechnology laboratories.
With competitive pricing, global shipping, and dedicated technical support, AAA Biotech makes it simple and convenient to source the high-quality recombinant proteins your work depends on.
FAQ
1. How are AAA Biotech recombinant proteins validated?
Each batch undergoes stringent quality control checks, including SDS-PAGE analysis, endotoxin testing (for select products), and activity assaying (for select products). Certificates of Analysis are provided with every product.
2. Are your proteins suitable for therapeutic development or only research?
AAA Biotech recombinant proteins are strictly for research-use only and are not intended for diagnostic or therapeutic purposes in humans or animals.
3. What types of expression systems do you use for recombinant protein production?
The production labs use a variety of expression platforms, including bacterial (E. coli), yeast, insect (baculovirus), and mammalian (HEK293, CHO) systems. The expression system used depends on the complexity and intended function/use of the protein.
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