At AAA Biotech, we offer a comprehensive selection of high-quality recombinant proteins for use in a wide range of research areas, including immunology, neuroscience, stem cell research, cancer research and more. No matter whether you need recombinant proteins for cell expansion, polarization, differentiation, or cell processing applications, we have got you covered.
Our recombinant proteins undergo rigorous quality testing. So, you can rely on AAA Biotech for high-quality recombinant proteins to support your research. Explore our catalog to find the right protein for your research needs.
Infected Trichoplusia ni cells are lysed and centrifuged and then the soluble extract containing the VLPs is dialyzed against Phosphate Buffered Saline.
Application Data (HTRF assay for JMJD1B / KDM3B activity 3.3 uM H3K9me2 peptide was incubated with different concentrations of JMJD1B / KDM3B protein in a 10 ul reaction system containing 50 mM HEPES pH 7.5, 1 mM TCEP, 0.02% Triton X100, 100 uM 2-oxoglutarate, 100 uM ascorbate, 50 ?M (NH4)2Fe(SO4)2·6H2O and for 1 hours at room temperature, then 10 ?l of H3K9me0 antibody and SA-XL665 mixture (1:100 dilution in the same buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Application Data (HTRF assay for JMJD1B / KDM3B activity 3.3 uM H3K9me2 peptide was incubated with different concentrations of JMJD1B / KDM3B protein in a 10 ul reaction system containing 50 mM HEPES pH 7.5, 1 mM TCEP, 0.02% Triton X100, 100 uM 2-oxoglutarate, 100 uM ascorbate, 50 ?M (NH4)2Fe(SO4)2·6H2O and for 1 hours at room temperature, then 10 ?l of H3K9me0 antibody and SA-XL665 mixture (1:100 dilution in the same buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
SDS-PAGE (Recombinant JMJD1B / KDM3B protein gel. JMJD1B / KDM3B protein was run on an 10% SDS-PAGE gel and stained with Coomassie blue. MW: 195 kDa Purity: >85%)
Application Data (Recombinant BRDT (257-382) HTRF activity assay. 3.3 uM histone peptide H4K5/8/12/16(4Ac) was incubated with BRDT (257-382) protein in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-GST antibody was used to detect reaction products.)
Application Data (Recombinant BRDT (257-382) HTRF activity assay. 3.3 uM histone peptide H4K5/8/12/16(4Ac) was incubated with BRDT (257-382) protein in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-GST antibody was used to detect reaction products.)
SDS-PAGE (Recombinant BRDT (257-382), GST-Tag protein gel. BRDT (257-382), GST-Tag protein was run on an SDS-PAGE gel and stained with Coomassie blue.)
Application Data (Recombinant KMT2D (MLL2) complex MALDI-TOF activity assay 3.3 uM H3K4me0 peptide was incubated with 10 nM KMT2D (MLL2) complex in reaction buffer for 3 hours at room temperature. The reaction product was detected by MALDI-TOF. Single 3.3 uM H3K4me0 peptide was used as negative control. Catalytic Ability: ~450 turnovers/ enzyme molecule)
Application Data (Recombinant KMT2D (MLL2) complex HTRF activity assay 3.3 uM H3K4me0 (1-21aa) peptide was incubated with KMT2D (MLL2) complex in reaction buffer for 3 hour at room temperature. KMT2D (MLL2) complex was used in a HTRF assay to determine enzyme linearity. Methylated peptide (H3K4me2) was measured using H3K4me2-specific antibody.)
SDS-PAGE (Recombinant KMT2D (MLL2) complex gel Recombinant KMT2D (MLL2) complex was run on an 10% SDS-PAGE gel and stained with Coomassie blue.)
Application Data (Recombinant HDAC6 (597-728, deleted mutant) protein activity assay. 3 ?M H3K9ac (1-21) peptides was incubated with different concentrations of HDAC6 (597-728, deleted mutant) in reaction buffer for 30 minutes at 37 degree C followed by developing for 30 minutes at room temperature.)
SDS-PAGE (Recombinant HDAC6 (597-728, deleted mutant) protein gel. HDAC6 protein was run on an 8% SDS-PAGE gel and stained with Coomassie blue.)
Application Data (MALDI-TOF for Recombinant PHD3 (EGLN3) protein, DYKDDDDK-Tag 3 ?M HIF1A peptide was incubated with 300 nM PHD3 protein in 30 ?l reaction system containing 20 mM Tris-HCl pH 7.5, 5 mM KCl, 1.5 mM MgCl2, 1 mM DTT, 100 ?M 2-oxoglutarate, 100 ?M ascorbate and 50 ?M (NH4)2Fe(SO4)2·6H2O for 2 hours at 30 degree C. MALDI-TOF was used for detection.)
Application Data (HTRF assay for JAK2 (532-1132, V617F) activity 1 uM TK substrate was incubated with different concentrations of JAK2 (532-1132, V617F) protein in 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT and 100 uM ATP for 1 hour. The detection reagents were added and incubated with the reactions for 1 hr. All the operations and reactions were performed at room temperature, and HTRF KinASE TK assay was used to detect the enzymatic activity.)
Application Data (HTRF assay for JAK2 (532-1132, V617F) activity 1 uM TK substrate was incubated with different concentrations of JAK2 (532-1132, V617F) protein in 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT and 100 uM ATP for 1 hour. The detection reagents were added and incubated with the reactions for 1 hr. All the operations and reactions were performed at room temperature, and HTRF KinASE TK assay was used to detect the enzymatic activity.)
SDS-PAGE (Recombinant JAK2 (532-1132, V617F) protein gel 10% SDS-PAGE gel with Coomassie blue staining MW: 71.1kDa Purity: >88%)
Application Data (HTRF assay for FAK protein activity 1 uM TK substrate was incubated with increasing concentrations of FAK protein and 100 uM ATP in reaction buffer for 1 hour. The detection reagents were added and incubated with the reactions for 1 hr. TK antibody was used to detect the products. All operations and reactions were performed at room temperature, and HTRF KinEASE TK assay was used to detect the enzymatic activity.)
Application Data (HTRF assay for FAK protein activity 1 uM TK substrate was incubated with increasing concentrations of FAK protein and 100 uM ATP in reaction buffer for 1 hour. The detection reagents were added and incubated with the reactions for 1 hr. TK antibody was used to detect the products. All operations and reactions were performed at room temperature, and HTRF KinEASE TK assay was used to detect the enzymatic activity. )
SDS-PAGE (Recombinant FAK protein gel 5% SDS-PAGE gel, stained with Coomassie Blue MW: 120.5 kDa Purity: >65% )
SDS-PAGE (Sortase A5 has increased labeling efficiency compared to the Wild-Type sortase. The H3K9Ac AbFlex antibody (67 ug) was labeled with 5 ug Biotin (1.28 mM) using 1 ug Sortase A5 or Wild-Type sortase at 30 degree C for 2 hr. with shaking. Top Panel: Following purification, 0.25 and 0.125 ug of labeled antibodies were run on an SDS-PAGE PAGE gel and labeling was detected using streptavidin-HRP. Lanes 1 & 2: 0.25 ug and 0.125 ug of H3K9Ac AbFlex Ab labeled with wild-type Sortase, respectively. Lanes 3 & 4: 0.25 ug and 0.125 ug of H3K9Ac AbFlex Ab labeled with Sortase A5, respectively. Bottom panel: The intensity of the labeling was quantified using BioRad’s Gel Doc imaging system, represented here as arbitrary units.)
SDS-PAGE (Sortase A5 protein gel. Sortase A5 run on an SDS-PAGE gel and stained with Coomassie Blue.)
Recombinant proteins are purified laboratory reagents produced through genetic engineering. A specific gene of interest is inserted into a host organism, such as mammalian, bacterial, yeast, or insect cells, which then expresses the protein in a controlled environment.
The recombinant process utilized to generate the recombinant proteins in our catalog provides precise control over sequence modifications, expression levels, and large-scale production tailored to experimental needs. These recombinants are widely used in research to investigate protein-protein interactions, enzyme activities, receptor-ligand binding, and cellular responses. Additionally, recombinant proteins serve as standards or controls in immunostaining assays and support cell growth and differentiation in culture systems, particularly in immunology, oncology, and structural biology studies.
Common Applications of Recombinant Proteins
Studying protein-protein and protein-DNA interactions
Functional assays to study biological pathways
Standard curves in ELISA and other quantitative assays
Use as antigens for antibody production
Development and screening of therapeutic drugs
Biomarker discovery and validation
Cell signaling and immune response studies
Vaccine research and development
Key Features of AAA Biotech’s Recombinant Proteins
High Purity: Most proteins are purified to ≥95% using affinity chromatography and other validated techniques.
Biological Activity: If functional activity is tested and confirmed for a given protein, it will be noted directly on the product page.
Multiple Expression Systems: Available in E. coli, HEK293, CHO, yeast, and insect cells to match your assay requirements.
Custom Tags Available: His-tag, GST, FLAG, and Fc fusion options for easy purification and detection.
Wide Range of Targets: Cytokines, growth factors, enzymes, receptors, signaling proteins, and more.
Validated Consistency: Lot-to-lot consistency ensured through rigorous QC protocols.
Flexible Quantities: Available in various pack sizes to suit different experimental needs.
Ready-to-Use Format: Lyophilized or liquid formulations that are easy to reconstitute and use.
Why Buy Recombinant Proteins from AAA Biotech?
At AAA Biotech, we are committed to supporting the research community with recombinant proteins that offer exceptional performance and reliability. Our proteins are produced using industry-standard methods and are validated to meet the needs of academic, pharmaceutical, and biotechnology laboratories.
With competitive pricing, global shipping, and dedicated technical support, AAA Biotech makes it simple and convenient to source the high-quality recombinant proteins your work depends on.
FAQ
1. How are AAA Biotech recombinant proteins validated?
Each batch undergoes stringent quality control checks, including SDS-PAGE analysis, endotoxin testing (for select products), and activity assaying (for select products). Certificates of Analysis are provided with every product.
2. Are your proteins suitable for therapeutic development or only research?
AAA Biotech recombinant proteins are strictly for research-use only and are not intended for diagnostic or therapeutic purposes in humans or animals.
3. What types of expression systems do you use for recombinant protein production?
The production labs use a variety of expression platforms, including bacterial (E. coli), yeast, insect (baculovirus), and mammalian (HEK293, CHO) systems. The expression system used depends on the complexity and intended function/use of the protein.
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