Rat Procollagen I N-Terminal Propeptide (PINP) ELISA Kit | PINP elisa kit

Rat Procollagen I N-Terminal Propeptide (PINP) Instant ELISA Kit

Cat. Num. AAA188625

• Gene Names: Col1a1; COLIA1
• Reactivity: Rat
• Applications: ELISA
• Synonyms: Procollagen I N-Terminal Propeptide (PINP); N/A; Rat Procollagen I N-Terminal Propeptide (PINP) Instant ELISA Kit; Procollagen I N-Terminal Propeptide; P1NP; N-Propeptide Of Type I Procollagen; Procollagen I Amino Terminal Propeptide; PINP elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Instant Procollagen I N-Terminal Propeptide (PINP).; No significant cross-reactivity or interference between Instant Procollagen I N-Terminal Propeptide (PINP) and analogues was observed.
• Sequence Length: 1453
• Assay Type: Double-antibody Sandwich
• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Detection Range: 246.9-20000pg/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 92.4pg/mL

• Applicable Applications for PINP elisa kit: ELISA
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Instant Procollagen I N-Terminal Propeptide (PINP) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Instant Procollagen I N-Terminal Propeptide (PINP) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Test Principle: The test principle applied in this kit is enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Instant Procollagen I N-Terminal Propeptide (PINP). Standards or samples and HRP-labeled detection antibody specific to Instant Procollagen I N-Terminal Propeptide (PINP) (Detection Reagent A) are then added to the appropriate microtiter plate wells. Next, TMB substrate solution is added, only those wells that contain Instant Procollagen I N-Terminal Propeptide (PINP), and HRP-labeled detection antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Instant Procollagen I N-Terminal Propeptide (PINP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Standard Curve (Sample)

NCBI and Uniprot Product Information

• NCBI GI #: 158711704
• NCBI GeneID: 29393
• NCBI Accession #: NP_445756.1
• NCBI GenBank Nucleotide #: NP_445756.1
• UniProt Accession #: P02454
• NCBI Official Full Name: collagen alpha-1(I) chain
• NCBI Official Synonym Full Names: collagen type I alpha 1 chain
• NCBI Official Symbol: Col1a1
• NCBI Official Synonym Symbols: COLIA1
• NCBI Protein Information: collagen alpha-1(I) chain
• UniProt Protein Name: Collagen alpha-1(I) chain
• UniProt Gene Name: Col1a1
• UniProt Entry Name: CO1A1_RAT