Rabbit anti-Human ZAP70 Polyclonal Antibody | anti-ZAP70 antibody

ZAP70 Antibody (Center)

Cat. Num. AAA290712

• Gene Names: ZAP70; SRK; STD; TZK; STCD; ZAP-70
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry, Western Blot
• Synonyms: ZAP70, Antibody; ZAP70 Antibody (Center); Tyrosine-protein kinase ZAP-70; 70 kDa zeta-chain associated protein; Syk-related tyrosine kinase; ZAP70; SRK; anti-ZAP70 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Ig
• Specificity: This ZAP70 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 252-282 amino acids from the Central region of human ZAP70.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Sequence Length: 619

• Applicable Applications for anti-ZAP70 antibody: ELISA, IHC (Immunohistochemistry), WB (Western Blot)
• Subtitle: Purified Rabbit Polyclonal Antibody (Pab)
• Cellular Location: Cytoplasm. Cell membrane; Peripheral membrane protein. Note=In quiescent T-lymphocytes, it is cytoplasmic. Upon TCR activation, it is recruited at the plasma membrane by interacting with CD247/CD3Z. Colocalizes together with RHOH in the immunological synapse. RHOH is required for its proper localization to the cell membrane and cytoskeleton fractions in the thymocytes (By similarity).
• Tissue Location: Expressed in T- and natural killer cells. Also present in early thymocytes and pro/pre B-cells.
• Antigen Region: 252-282 aa
• Antigen Type: Synthetic Peptide
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

IHC (Immunohiostchemistry)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

WB (Western Blot)

(All lanes : Anti-ZAP70 Antibody (A267) at 1:1000 dilutionLane 1: Jurkat whole cell lysateLane 2: MOLT-4 whole cell lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 70 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-ZAP70 antibody

Description: This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene.

Background: Tyrosine kinase that plays an essential role in regulation of the adaptive immune response. Regulates motility, adhesion and cytokine expression of mature T-cells, as well as thymocyte development. Contributes also to the development and activation of primary B-lymphocytes. When antigen presenting cells (APC) activate T-cell receptor (TCR), a serie of phosphorylations lead to the recruitment of ZAP70 to the doubly phosphorylated TCR component CD247/CD3Z through ITAM motif at the plasma membrane. This recruitment serves to localization to the stimulated TCR and to relieve its autoinhibited conformation. Release of ZAP70 active conformation is further stabilized by phosphorylation mediated by LCK. Subsequently, ZAP70 phosphorylates at least 2 essential adapter proteins: LAT and LCP2. In turn, a large number of signaling molecules are recruited and ultimately lead to lymphokine production, T-cell proliferation and differentiation. Furthermore, ZAP70 controls cytoskeleton modifications, adhesion and mobility of T-lymphocytes, thus ensuring correct delivery of effectors to the APC. ZAP70 is also required for TCR-CD247/CD3Z internalization and degradation through interaction with the E3 ubiquitin-protein ligase CBL and adapter proteins SLA and SLA2. Thus, ZAP70 regulates both T-cell activation switch on and switch off by modulating TCR expression at the T-cell surface. During thymocyte development, ZAP70 promotes survival and cell-cycle progression of developing thymocytes before positive selection (when cells are still CD4/CD8 double negative). Additionally, ZAP70-dependent signaling pathway may also contribute to primary B-cells formation and activation through B-cell receptor (BCR).

Product Categories/Family for anti-ZAP70 antibody

Primary Antibodies; Immunology; Signal Transduction

References

Kuroyama, H., et al., Biochem. Biophys. Res. Commun. 315 (4):935-941 (2004). Yang, Y., et al., Mol. Cell. Biol. 23 (21):7667-7677 (2003). Kuhne, M.R., et al., J. Immunol. 171 (2):860-866 (2003). Kremer, K.N., et al., J. Immunol. 171 (1):360-367 (2003). Wiestner, A., et al., Blood 101 (12):4944-4951 (2003).

NCBI and Uniprot Product Information

• NCBI GI #: 31455611
• NCBI GeneID: 7535
• NCBI Accession #: NP_001070.2
• NCBI GenBank Nucleotide #: NM_001079.3
• UniProt Accession #: P43403; Q6PIA4; Q8IXD6; Q9UBS6; A6NFP4
• Molecular Weight: 69872 Da
• NCBI Official Full Name: tyrosine-protein kinase ZAP-70 isoform 1
• NCBI Official Synonym Full Names: zeta chain of T cell receptor associated protein kinase 70kDa
• NCBI Official Symbol: ZAP70
• NCBI Official Synonym Symbols: SRK; STD; TZK; STCD; ZAP-70
• NCBI Protein Information: tyrosine-protein kinase ZAP-70
• UniProt Protein Name: Tyrosine-protein kinase ZAP-70
• UniProt Gene Name: ZAP70
• UniProt Synonym Gene Names: SRK
• UniProt Entry Name: ZAP70_HUMAN