WB (Western Blot)
(Detection of human and mouse UCH-L3 by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug) and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-UCH-L3 antibody AAA212079 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. UCH-L3 was also immunoprecipitated by rabbit anti-UCH-L3 antibody which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 3 seconds (B).)
Rabbit anti-Human, Mouse UCH-L3 Polyclonal Antibody | anti-UCH-L3 antibody
Rabbit anti-UCH-L3 Antibody, Affinity Purified
Gene Names
UCHL3; UCH-L3
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
UCH-L3, Antibody; Rabbit anti-UCH-L3 Antibody, Affinity Purified; UCH-L3; ubiquitin thiolesterase; ubiquitin thioesterase L3; ubiquitin carboxyl-terminal hydrolase isozyme L3; testicular tissue protein Li 221; ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase); Ubiquitin carboxyl-terminal hydrolase isozyme L3; ubiquitin C-terminal hydrolase L3; anti-UCH-L3 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-UCH-L3 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Rat, Bovine, Pig
Immunogen
Between 180 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human and mouse UCH-L3 by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug) and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-UCH-L3 antibody AAA212079 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. UCH-L3 was also immunoprecipitated by rabbit anti-UCH-L3 antibody which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 3 seconds (B).)
WB (Western Blot)
(Detection of human and mouse UCH-L3 by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug) and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-UCH-L3 antibody AAA212079 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. UCH-L3 was also immunoprecipitated by rabbit anti-UCH-L3 antibody which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 3 seconds (B).)
IHC (Immunohistochemistry)
(Detection of human UCH-L3 by immunohistochemistry. Sample: FFPE section of human testicular seminoma. Antibody: Affinity purified rabbit anti-UCH-L3 (Cat. No. AAA212079 Lot1) used at a dilution of 1:200 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human UCH-L3 by immunohistochemistry. Sample: FFPE section of human testicular seminoma. Antibody: Affinity purified rabbit anti-UCH-L3 (Cat. No. AAA212079 Lot1) used at a dilution of 1:200 (1ug/ml). Detection: DAB)
Related Product Information for anti-UCH-L3 antibody
Ubiquitin carboxyl-terminal hydrolase isoenzyme 3 (UCH-L3) is a deubiquitinating enzyme (DUB) and thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8. UCH-L3 deubiquitinates ENAC in apical compartments, thereby regulating apical membrane recycling [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P15374].
Product Categories/Family for anti-UCH-L3 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
ubiquitin carboxyl-terminal hydrolase isozyme L3 isoform 2
NCBI Official Synonym Full Names
ubiquitin C-terminal hydrolase L3
NCBI Official Symbol
UCHL3
NCBI Official Synonym Symbols
UCH-L3
NCBI Protein Information
ubiquitin carboxyl-terminal hydrolase isozyme L3
UniProt Protein Name
Ubiquitin carboxyl-terminal hydrolase isozyme L3
UniProt Gene Name
UCHL3
UniProt Synonym Gene Names
UCH-L3
UniProt Entry Name
UCHL3_HUMAN
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Product Notes
The UCH-L3 uchl3 (Catalog #AAA212079) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-UCH-L3 Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's UCH-L3 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the UCH-L3 uchl3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "UCH-L3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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