WB (Western Blot)
(Western blot analysis of UBE2M Antibody expression in 293T cells lysates.)
Rabbit UBE2M Polyclonal Antibody | anti-UBE2M antibody
UBE2M Antibody
Gene Names
UBE2M; UBC12; hUbc12; UBC-RS2
Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
UBE2M, Antibody; UBE2M Antibody; hUbc12; NEDD8 carrier protein; NEDD8 conjugating enzyme Ubc12; NEDD8 protein ligase; NEDD8-conjugating enzyme Ubc12; UBC-RS2; UBC12; UBC12_HUMAN; ube2m; ubiquitin carrier protein M; Ubiquitin conjugating enzyme E2 M; Ubiquitin-conjugating enzyme E2 M; ubiquitin-protein ligase M; yeast UBC12 homolog; anti-UBE2M antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
UBE2M antibody detects endogenous levels of total UBE2M
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
183
Applicable Applications for anti-UBE2M antibody
ELISA, IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide derived from human UBE2M
Predicted Cross Reactivity
Pig, Bovine, Horse, Rabbit, Dog
Similarity
Pig (100%), Bovine (100%), Horse (100%), Rabbit (100%), Dog (100%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
WB (Western Blot)
(Western blot analysis of UBE2M Antibody expression in 293T cells lysates.)
WB (Western Blot)
(Western blot analysis of UBE2M Antibody expression in 293T cells lysates.)
IHC (Immunohiostchemistry)
(AAA327430 at 1/100 staining Mouse lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IHC (Immunohiostchemistry)
(AAA327430 at 1/100 staining Mouse lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
WB (Western Blot)
(Western blot analysis UBE2M using COLO205 whole cell lysates)
WB (Western Blot)
(Western blot analysis UBE2M using COLO205 whole cell lysates)
Related Product Information for anti-UBE2M antibody
Function: Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX1, but not RBX2, suggests that the RBX1-UBE2M complex neddylates specific target proteins, such as CUL1, CUL2, CUL3 and CUL4. Involved in cell proliferation.
Subunit Structure: Interacts with UBA3 and RBX1. Interacts (acetylated at N-terminal methionine) with DCUN1D1 (via DCUN1 domain) (PubMed:28581483).
Post-translational Modifications: The acetylation of Met-1 increases affinity for DCUN1D1 by about 2 orders of magnitude and is crucial for NEDD8 transfer to cullins.
Similarity: Both the N-terminal docking peptide and the catalytic core domain must bind the UBA3-NAE1 complex simultaneously for optimal transfer of NEDD8. Belongs to the ubiquitin-conjugating enzyme family. UBC12 subfamily.
Subunit Structure: Interacts with UBA3 and RBX1. Interacts (acetylated at N-terminal methionine) with DCUN1D1 (via DCUN1 domain) (PubMed:28581483).
Post-translational Modifications: The acetylation of Met-1 increases affinity for DCUN1D1 by about 2 orders of magnitude and is crucial for NEDD8 transfer to cullins.
Similarity: Both the N-terminal docking peptide and the catalytic core domain must bind the UBA3-NAE1 complex simultaneously for optimal transfer of NEDD8. Belongs to the ubiquitin-conjugating enzyme family. UBC12 subfamily.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 21 kDa
Predicted: 21 kDa
Predicted: 21 kDa
NCBI Official Full Name
NEDD8-conjugating enzyme Ubc12
NCBI Official Synonym Full Names
ubiquitin conjugating enzyme E2 M
NCBI Official Symbol
UBE2M
NCBI Official Synonym Symbols
UBC12; hUbc12; UBC-RS2
NCBI Protein Information
NEDD8-conjugating enzyme Ubc12
UniProt Protein Name
NEDD8-conjugating enzyme Ubc12
UniProt Gene Name
UBE2M
UniProt Synonym Gene Names
UBC12
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Product Notes
The UBE2M ube2m (Catalog #AAA327430) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The UBE2M Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's UBE2M can be used in a range of immunoassay formats including, but not limited to, ELISA, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the UBE2M ube2m for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "UBE2M, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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