IHC (Immunohistochemisry)
(TSN Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TSN Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Rabbit TSN Polyclonal Antibody | anti-TSN antibody
TSN Antibody (Center)
Gene Names
TSN; C3PO; RCHF1; TBRBP; TRSLN; BCLF-1; REHF-1
Reactivity
Human, mouse (Predicted Reactivity: Bovine, Hamster)
Applications
Western Blot, Immunofluorescence, Immunohistochemistry, ELISA
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
TSN, Antibody; TSN Antibody (Center); Translin; 31--; Component 3 of promoter of RISC; C3PO; TSN; anti-TSN antibody
Host
Rabbit
Reactivity
Human, mouse (Predicted Reactivity: Bovine, Hamster)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This TSN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 109-138 amino acids from the Central region of human TSN.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
109-138
Sequence Length
148
Applicable Applications for anti-TSN antibody
WB (Western Blot), IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA
Antigen Type
Synthetic Peptide
Crown Antibody
Yes
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohistochemisry)
(TSN Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TSN Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohistochemisry)
(TSN Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TSN Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
IF (Immunofluorescence)
(Fluorescent confocal image of Hela cell stained with TSN Antibody(Center). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TSN primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).TSN immunoreactivity is localized to Nucleus and Cytoplasm significantly.)
IF (Immunofluorescence)
(Fluorescent confocal image of Hela cell stained with TSN Antibody(Center). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TSN primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).TSN immunoreactivity is localized to Nucleus and Cytoplasm significantly.)
WB (Western Blot)
(TSN Antibody (Center) western blot analysis in HL-60,Jurkat,K562 cell line mouse spleen tissue lysates (35ug/lane).This demonstrates the TSN antibody detected the TSN protein (arrow).)
WB (Western Blot)
(TSN Antibody (Center) western blot analysis in HL-60,Jurkat,K562 cell line mouse spleen tissue lysates (35ug/lane).This demonstrates the TSN antibody detected the TSN protein (arrow).)
Related Product Information for anti-TSN antibody
This gene encodes a DNA-binding protein which specifically
recognizes conserved target sequences at the breakpoint junction of
chromosomal translocations. Translin polypeptides form a multimeric
structure that is responsible for its DNA-binding activity.
Recombination-associated motifs and translin-binding sites are
present at recombination hotspots and may serve as indicators of
breakpoints in genes which are fused by translocations. These
binding activities may play a crucial role in chromosomal
translocation in lymphoid neoplasms.
recognizes conserved target sequences at the breakpoint junction of
chromosomal translocations. Translin polypeptides form a multimeric
structure that is responsible for its DNA-binding activity.
Recombination-associated motifs and translin-binding sites are
present at recombination hotspots and may serve as indicators of
breakpoints in genes which are fused by translocations. These
binding activities may play a crucial role in chromosomal
translocation in lymphoid neoplasms.
Product Categories/Family for anti-TSN antibody
References
Chiaruttini, C., et al. Proc. Natl. Acad. Sci. U.S.A. 106(38):16481-16486(2009)
Lasky-Su, J., et al. Am. J. Med. Genet. B Neuropsychiatr. Genet. 147B (8), 1345-1354 (2008) :
Sengupta, K., et al. Biochemistry 45(3):861-870(2006)
Kaluzhny, D., et al. J. Biomol. Struct. Dyn. 23(3):257-265(2005)
Gupta, G.D., et al. FEBS Lett. 579(14):3141-3146(2005)
Lasky-Su, J., et al. Am. J. Med. Genet. B Neuropsychiatr. Genet. 147B (8), 1345-1354 (2008) :
Sengupta, K., et al. Biochemistry 45(3):861-870(2006)
Kaluzhny, D., et al. J. Biomol. Struct. Dyn. 23(3):257-265(2005)
Gupta, G.D., et al. FEBS Lett. 579(14):3141-3146(2005)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
26183
NCBI Official Full Name
translin isoform 2
NCBI Official Synonym Full Names
translin
NCBI Official Symbol
TSN
NCBI Official Synonym Symbols
C3PO; RCHF1; TBRBP; TRSLN; BCLF-1; REHF-1
NCBI Protein Information
translin
UniProt Protein Name
Translin
UniProt Gene Name
TSN
UniProt Synonym Gene Names
C3PO
UniProt Entry Name
TSN_HUMAN
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Product Notes
The TSN tsn (Catalog #AAA290131) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 109-138. The TSN Antibody (Center) reacts with Human, mouse (Predicted Reactivity: Bovine, Hamster) and may cross-react with other species as described in the data sheet. AAA Biotech's TSN can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA. Researchers should empirically determine the suitability of the TSN tsn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TSN, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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