WB (Western Blot)
(Western blot analysis of lysates from U-87 MG, SH-SY5Y cell line, human brain, mouse brain, rat brain tissue lysate (from left to right), using STMN2 Antibody (N-term). AAA290010 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
Rabbit STMN2 Polyclonal Antibody | anti-STMN2 antibody
STMN2 Antibody (N-term)
Gene Names
STMN2; SCG10; SCGN10
Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Synonyms
STMN2, Antibody; STMN2 Antibody (N-term); Stathmin-2; Superior cervical ganglion-10 protein; Protein SCG10; STMN2; SCG10; SCGN10; anti-STMN2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This STMN2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 36-69 amino acids from the N-terminal region of human STMN2.
Purity/Purification
This antibody is purified through a protein A column, followed by peptide affinity purification.
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Applicable Applications for anti-STMN2 antibody
ELISA, WB (Western Blot), IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry)
Cellular Location
Cytoplasm. Cytoplasm, perinuclear region. Cell projection, growth cone Membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, axon. Golgi apparatus. Endosome. Cell projection, lamellipodium Note=Associated with punctate structures in the perinuclear cytoplasm, axons, and growth cones of developing neurons. SCG10 exists in both soluble and membrane-bound forms. Colocalized with CIB1 in neurites of developing hippocampal primary neurons (By similarity). Colocalized with CIB1 in the cell body, neuritis and growth cones of neurons. Colocalized with CIB1 to the leading edge of lamellipodia.
Tissue Location
Neuron specific.
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(Western blot analysis of lysates from U-87 MG, SH-SY5Y cell line, human brain, mouse brain, rat brain tissue lysate (from left to right), using STMN2 Antibody (N-term). AAA290010 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
WB (Western Blot)
(Western blot analysis of lysates from U-87 MG, SH-SY5Y cell line, human brain, mouse brain, rat brain tissue lysate (from left to right), using STMN2 Antibody (N-term). AAA290010 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of paraffin-embedded H. liver section using STMN2 Antibody (N-term). AAA290010 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of paraffin-embedded H. liver section using STMN2 Antibody (N-term). AAA290010 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of SH-SY5Y cells using STMN2 Antibody (N-term)(green) compared to an isotype control of rabbit IgG(blue). AAA290010 was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of SH-SY5Y cells using STMN2 Antibody (N-term)(green) compared to an isotype control of rabbit IgG(blue). AAA290010 was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.)
Related Product Information for anti-STMN2 antibody
Regulator of microtubule stability. When phosphorylated by MAPK8, stabilizes microtubules and consequently controls neurite length in cortical neurons. In the developing brain, negatively regulates the rate of exit from multipolar stage and retards radial migration from the ventricular zone (By similarity).
Product Categories/Family for anti-STMN2 antibody
References
Okazaki T.,et al.Neurobiol. Aging 16:883-894(1995).
Fujiwara T.,et al.Submitted (APR-1995) to the EMBL/GenBank/DDBJ databases.
Kalnine N.,et al.Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
Ebert L.,et al.Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Fujiwara T.,et al.Submitted (APR-1995) to the EMBL/GenBank/DDBJ databases.
Kalnine N.,et al.Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
Ebert L.,et al.Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
20828
NCBI Official Full Name
stathmin-2 isoform 1
NCBI Official Synonym Full Names
stathmin 2
NCBI Official Symbol
STMN2
NCBI Official Synonym Symbols
SCG10; SCGN10
NCBI Protein Information
stathmin-2
UniProt Protein Name
Stathmin-2
UniProt Gene Name
STMN2
UniProt Synonym Gene Names
SCG10; SCGN10; Protein SCG10
UniProt Entry Name
STMN2_HUMAN
Similar Products
Product Notes
The STMN2 stmn2 (Catalog #AAA290010) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The STMN2 Antibody (N-term) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's STMN2 can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot), IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the STMN2 stmn2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "STMN2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.