Rabbit SORBS1 Polyclonal Antibody | anti-SORBS1 antibody

SORBS1 Antibody (Center)

Cat. Num. AAA286213

• Gene Names: SORBS1; CAP; FLAF2; R85FL; SH3D5; SORB1; SH3P12
• Reactivity: Human, Hamster; (Predicted Reactivity: Mouse)
• Applications: Immunofluorescence, ELISA, Western Blot
• Purity: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Synonyms: SORBS1, Antibody; SORBS1 Antibody (Center); Sorbin and SH3 domain-containing protein 1; Ponsin; SH3 domain protein 5; SH3P12; c-Cbl-associated protein; CAP; SORBS1 HGNC:14565); anti-SORBS1 antibody

• Host: Rabbit
• Reactivity: Human, Hamster; (Predicted Reactivity: Mouse)
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This SORBS1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 866-892 amino acids from the Central region of human SORBS1.
• Purity/Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Sequence Positions: 866-892
• Sequence Length: 1,294

• Applicable Applications for anti-SORBS1 antibody: IF (Immunofluorescence), ELISA, WB (Western Blot)
• Function: Plays a role in tyrosine phosphorylation of CBL by linking CBL to the insulin receptor. Required for insulin-stimulated glucose transport. Involved in formation of actin stress fibers and focal adhesions (By similarity).
• Cellular Location: Cell junction, adherens junction. Cell membrane. Cytoplasm, cytoskeleton. Cell junction, focal adhesion. Nucleus. Nucleus matrix. Note=Colocalizes with the Ten-1 ICD form of TENM1 in the nucleus (By similarity). Colocalizes with actin stress fibers. Also detected at the plasma membrane and in neuronal intranuclear inclusions. Colocalized with PXN at focal adhesions during myogenic differentiation.
• Tissue Location: Detected in skeletal muscle (at protein level). Widely expressed with highest levels in heart and skeletal muscle
• Preparation and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

IF (Immunofluorescence)

(Confocal immunofluorescent analysis of SORBS1 Antibody (Center) with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

WB (Western Blot)

(SORBS1 Antibody (Center) western blot analysis in CHO cell line lysates (35ug/lane).This demonstrates the SORBS1 antibody detected the SORBS1 protein (arrow).)

WB (Western Blot)

(SORBS1 Antibody (Center) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the SORBS1 antibody detected the SORBS1 protein (arrow).)

Product Categories/Family for anti-SORBS1 antibody

Signal Transduction

References

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) : Joslyn, G., et al. Alcohol. Clin. Exp. Res. 34(5):800-812(2010) Gehmlich, K., et al. Eur. J. Cell Biol. 89(5):351-364(2010) Yamada, Y., et al. Hypertens. Res. 32(5):325-331(2009) Fernow, I., et al. BMC Cell Biol. 10, 80 (2009) :

NCBI and Uniprot Product Information

• NCBI GI #: 78000163
• NCBI GeneID: 10580
• NCBI Accession #: NP_001030126.1; NP_001030127.1; NP_001030128.1; NP_001030129.1; NP_006425.2; NP_056200.1; NP_079267.1
• NCBI GenBank Nucleotide #: NM_001034954.1
• UniProt Accession #: Q9BX66; O43857; Q5T923; Q5T924; Q5T927; Q5T928; A0AED4; A6NEK3; A6NID8; A6NJS4; A7MD40; D3DR42
• Molecular Weight: 142513
• NCBI Official Full Name: sorbin and SH3 domain-containing protein 1 isoform 3
• NCBI Official Synonym Full Names: sorbin and SH3 domain containing 1
• NCBI Official Symbol: SORBS1
• NCBI Official Synonym Symbols: CAP; FLAF2; R85FL; SH3D5; SORB1; SH3P12
• NCBI Protein Information: sorbin and SH3 domain-containing protein 1
• UniProt Protein Name: Sorbin and SH3 domain-containing protein 1
• UniProt Gene Name: SORBS1
• UniProt Synonym Gene Names: CAP
• UniProt Entry Name: SRBS1_HUMAN