WB (Western Blot)
(Detection of human and mouse SMYD2 by western blot. Samples: Whole cell lysate (50 & 15 ug) from HeLa, (50 ug) from HEK293T, Jurkat, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SMYD2 antibody AAA212095 (lot AAA212095-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Rabbit anti-Human, Mouse SMYD2 Polyclonal Antibody | anti-SMYD2 antibody
Rabbit anti-SMYD2 Antibody, Affinity Purified
Gene Names
SMYD2; KMT3C; HSKM-B; ZMYND14
Reactivity
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
SMYD2, Antibody; Rabbit anti-SMYD2 Antibody, Affinity Purified; ZMYND14; histone methyltransferase SMYD2; HSKM-B; KMT3C; lysine N-methyltransferase 3C; N-lysine methyltransferase SMYD2; SET and MYND domain-containing protein 2; zinc finger, MYND domain containing 14; SET and MYND domain containing 2; anti-SMYD2 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-SMYD2 antibody
IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Chicken, Bovine, Pig
Immunogen
Between 383 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human and mouse SMYD2 by western blot. Samples: Whole cell lysate (50 & 15 ug) from HeLa, (50 ug) from HEK293T, Jurkat, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SMYD2 antibody AAA212095 (lot AAA212095-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of human and mouse SMYD2 by western blot. Samples: Whole cell lysate (50 & 15 ug) from HeLa, (50 ug) from HEK293T, Jurkat, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SMYD2 antibody AAA212095 (lot AAA212095-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human SMYD2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SMYD2 antibody AAA212095 (lot AAA212095-2) used for IP at 6 ug per reaction. SMYD2 was also immunoprecipitated by a previous lot of this antibody (lot AAA212095-1) and rabbit anti-SMYD2 antibody BL10995. For blotting immunoprecipitated SMYD2, AAA212095 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human SMYD2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SMYD2 antibody AAA212095 (lot AAA212095-2) used for IP at 6 ug per reaction. SMYD2 was also immunoprecipitated by a previous lot of this antibody (lot AAA212095-1) and rabbit anti-SMYD2 antibody BL10995. For blotting immunoprecipitated SMYD2, AAA212095 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Related Product Information for anti-SMYD2 antibody
SET- and MYND-containing protein 2 (SMYD2) functions as a histone methyltransferase and has been reported to methylate p53 and histone H3.
Product Categories/Family for anti-SMYD2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
50
NCBI Official Full Name
N-lysine methyltransferase SMYD2
NCBI Official Synonym Full Names
SET and MYND domain containing 2
NCBI Official Symbol
SMYD2
NCBI Official Synonym Symbols
KMT3C; HSKM-B; ZMYND14
NCBI Protein Information
N-lysine methyltransferase SMYD2
UniProt Protein Name
N-lysine methyltransferase SMYD2
UniProt Gene Name
SMYD2
UniProt Synonym Gene Names
KMT3C
UniProt Entry Name
SMYD2_HUMAN
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Product Notes
The SMYD2 smyd2 (Catalog #AAA212095) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-SMYD2 Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's SMYD2 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the SMYD2 smyd2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SMYD2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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