IHC (Immunohiostchemistry)
(SIGLEC8 Antibody (N-term) (AAA289969)immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SIGLEC8 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Rabbit anti-Human SIGLEC8 Polyclonal Antibody | anti-SIGLEC8 antibody
SIGLEC8 Antibody (N-term)
Gene Names
SIGLEC8; SAF2; SIGLEC-8; SIGLEC8L
Reactivity
Human
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
SIGLEC8, Antibody; SIGLEC8 Antibody (N-term); Sialic acid-binding Ig-like lectin 8; Siglec-8; Sialoadhesin family member 2; SAF-2; SIGLEC8; SAF2; anti-SIGLEC8 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This SIGLEC8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 94-122 amino acids from the N-terminal region of human SIGLEC8.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
94-122
Sequence Length
431
Applicable Applications for anti-SIGLEC8 antibody
IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohiostchemistry)
(SIGLEC8 Antibody (N-term) (AAA289969)immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SIGLEC8 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(SIGLEC8 Antibody (N-term) (AAA289969)immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SIGLEC8 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(SIGLEC8 Antibody (N-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the SIGLEC8 antibody detected the SIGLEC8 protein (arrow).)
WB (Western Blot)
(SIGLEC8 Antibody (N-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the SIGLEC8 antibody detected the SIGLEC8 protein (arrow).)
Related Product Information for anti-SIGLEC8 antibody
Sialic acid-binding immunoglobulin (Ig)-like lectins, or
SIGLECs (e.g., CD33 (MIM 159590)), are a family of type 1
transmembrane proteins each having a unique expression pattern,
mostly in hemopoietic cells. SIGLEC8 is a member of the CD33-like
subgroup of SIGLECs, which are localized to 19q13.3-q13.4 and have
2 conserved cytoplasmic tyrosine-based motifs: an immunoreceptor
tyrosine-based inhibitory motif, or ITIM (see MIM 604964), and a
motif homologous to one identified in signaling lymphocyte
activation molecule (SLAM; MIM 603492) that mediates an association
with SLAM-associated protein (SAP; MIM 300490) (summarized by
Foussias et al., 2000 [PubMed 11095983]).
SIGLECs (e.g., CD33 (MIM 159590)), are a family of type 1
transmembrane proteins each having a unique expression pattern,
mostly in hemopoietic cells. SIGLEC8 is a member of the CD33-like
subgroup of SIGLECs, which are localized to 19q13.3-q13.4 and have
2 conserved cytoplasmic tyrosine-based motifs: an immunoreceptor
tyrosine-based inhibitory motif, or ITIM (see MIM 604964), and a
motif homologous to one identified in signaling lymphocyte
activation molecule (SLAM; MIM 603492) that mediates an association
with SLAM-associated protein (SAP; MIM 300490) (summarized by
Foussias et al., 2000 [PubMed 11095983]).
Product Categories/Family for anti-SIGLEC8 antibody
References
Gao, P.S., et al. Eur. J. Hum. Genet. 18(6):713-719(2010)
Davila, S., et al. Genes Immun. 11(3):232-238(2010)
Hudson, S.A., et al. J. Pharmacol. Exp. Ther. 330(2):608-612(2009)
Bochner, B.S. Clin. Exp. Allergy 39(3):317-324(2009)
von Gunten, S., et al. Autoimmun Rev 7(6):453-456(2008)
Davila, S., et al. Genes Immun. 11(3):232-238(2010)
Hudson, S.A., et al. J. Pharmacol. Exp. Ther. 330(2):608-612(2009)
Bochner, B.S. Clin. Exp. Allergy 39(3):317-324(2009)
von Gunten, S., et al. Autoimmun Rev 7(6):453-456(2008)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
54042
NCBI Official Full Name
sialic acid-binding Ig-like lectin 8
NCBI Official Synonym Full Names
sialic acid binding Ig-like lectin 8
NCBI Official Symbol
SIGLEC8
NCBI Official Synonym Symbols
SAF2; SIGLEC-8; SIGLEC8L
NCBI Protein Information
sialic acid-binding Ig-like lectin 8
UniProt Protein Name
Sialic acid-binding Ig-like lectin 8
UniProt Gene Name
SIGLEC8
UniProt Synonym Gene Names
SAF2; Siglec-8; SAF-2
UniProt Entry Name
SIGL8_HUMAN
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Product Notes
The SIGLEC8 siglec8 (Catalog #AAA289969) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 94-122. The SIGLEC8 Antibody (N-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SIGLEC8 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the SIGLEC8 siglec8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SIGLEC8, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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