Rabbit anti-Human, mouse SIGLEC5 Polyclonal Antibody | anti-SIGLEC5 antibody

SIGLEC5 Antibody (N-term)

Cat. Num. AAA287431

• Gene Names: SIGLEC5; CD170; OBBP2; CD33L2; OB-BP2; SIGLEC-5
• Reactivity: Human, mouse
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: SIGLEC5, Antibody; SIGLEC5 Antibody (N-term); Sialic acid-binding Ig-like lectin 5; Siglec-5; CD33 antigen-like 2; Obesity-binding protein 2; OB-BP2; OB-binding protein 2; CD170; SIGLEC5; CD33L2; OBBP2; anti-SIGLEC5 antibody

• Host: Rabbit
• Reactivity: Human, mouse
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This SIGLEC5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 8-39 amino acids from the N-terminal region of human SIGLEC5.
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Concentration: Vial Concentration: 2 (varies by lot)
• Sequence Positions: 8-39
• Sequence Length: 551

• Applicable Applications for anti-SIGLEC5 antibody: IHC (Immunohistochemistry), ELISA, WB (Western Blot)
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

WB (Western Blot)

(SIGLEC5 Antibody (S23) western blot analysis in T47D cell line lysates (35ug/lane).This demonstrates the SIGLEC5 antibody detected the SIGLEC5 protein (arrow).)

IHC (Immunohistochemisry)

(Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with SIGLEC5 Antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

IHC (Immunohiostchemistry)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

WB (Western Blot)

(The anti-Siglec5 N-term Pab is used in Western blot to detect Siglec5 in mouse liver tissue lysate.)

Related Product Information for anti-SIGLEC5 antibody

SIGLEC5 is a putative adhesion molecule that mediates sialic-acid dependent binding to cells. This protein binds equally to alpha2,3-linked and alpha2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. This molecule is expressed by monocytic/myeloid lineage cells. SIGLEC5 is found at high levels in peripheral blood leukocytes, spleen, bone marrow and at lower levels in lymph node, lung, appendix, placenta, pancreas and thymus. It is expressed by monocytes and neutrophils but absent from leukemic cell lines representing early stages of myelomonocytic differenciation.

Product Categories/Family for anti-SIGLEC5 antibody

Immunology

References

Erickson-Miller, C.L., et al., Exp. Hematol. 31(5):382-388 (2003).
Patel, N., et al., J. Biol. Chem. 274(32):22729-22738 (1999).
Cornish, A.L., et al., Blood 92(6):2123-2132 (1998).
Kim, H.S., Cytogenet. Cell Genet. 84 (1-2), 96 (1999).

NCBI and Uniprot Product Information

• NCBI GI #: 4502659
• NCBI GeneID: 8778
• NCBI Accession #: NP_003821.1
• NCBI GenBank Nucleotide #: NM_003830.3
• UniProt Accession #: O15389
• Molecular Weight: 60715
• NCBI Official Full Name: sialic acid-binding Ig-like lectin 5
• NCBI Official Synonym Full Names: sialic acid binding Ig-like lectin 5
• NCBI Official Symbol: SIGLEC5
• NCBI Official Synonym Symbols: CD170; OBBP2; CD33L2; OB-BP2; SIGLEC-5
• NCBI Protein Information: sialic acid-binding Ig-like lectin 5
• UniProt Protein Name: Sialic acid-binding Ig-like lectin 5
• UniProt Gene Name: SIGLEC5
• UniProt Synonym Gene Names: CD33L2; OBBP2; Siglec-5; OB-BP2; OB-binding protein 2
• UniProt Entry Name: SIGL5_HUMAN