Rabbit SFN Polyclonal Antibody | anti-SFN antibody

SFN Antibody

Cat. Num. AAA304947

• Gene Names: SFN; YWHAS
• Reactivity: Human, Mouse, Rat
• Applications: Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: Antibodies were purified by affinity purification using immunogen.
• Synonyms: SFN, Antibody; SFN Antibody; SFN; YWHAS; anti-SFN antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total SFN protein.
• Purity/Purification: Antibodies were purified by affinity purification using immunogen.
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 248

• Applicable Applications for anti-SFN antibody: IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
• Immunogen Type: Recombinant Protein
• Immunogen Description: Recombinant protein of human SFN.
• Target Name: SFN
• Preparation and Storage: Store at -20 degree C

IF (Immunofluorescence)

(Immunofluorescence analysis of MCF7 cell using SFN antibody. Blue: DAPI for nuclear staining.)

IHC (Immunohistochemisry)

(Immunohistochemical analysis of paraffin-embedded rat kidney using SFN antibody at dilution of 1:100 (400x lens).)

IHC (Immunohiostchemistry)

(Immunohistochemical analysis of paraffin-embedded mouse kidney using SFN antibody at dilution of 1:100 (400x lens).)

WB (Western Blot)

(Western blot analysis of extracts of various cell lines, using SFN antibody.)

Related Product Information for anti-SFN antibody

14-3-3 proteins regulate many cellular processes relevant to cancer biology, notably apoptosis, mitogenic signaling and cell-cycle checkpoints. Seven isoforms, denoted 14-3-3 b, g, e, z, h, q and s, comprise this family of signaling intermediates. 14-3-3 s, also known as SFN, stratifin, HME1 or YWHAS, is a secreted adaptor protein that is involved in regulating both general and specific signaling pathways. Expressed predominately in stratified squamous keratinising epithelium, 14-3-3 s is able to bind and modify the activity of a large number of proteins, such as KRT17 (Keratin 17), through recognition of a phosphothreonine or phosphoserine motif. When bound to Keratin 17, for example, 14-3-3 s acts to stimulate the Akt/mTOR signaling pathway by upregulating protein synthesis and cell growth. 14-3-3 s also functions to positively mediate IGF-I-induced cell cycle progression and can bind to a variety of translation initiation factors, thus controlling mitotic translation. In response to tumor growth, 14-3-3 s positively regulates the tumor suppressor p53 and increases the rate of p53-regulated inhibition of G2/M cell cycle progression. Multiple isoforms of 14-3-3 s exist due to alternative splicing events.

Product Categories/Family for anti-SFN antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 5454052
• NCBI GeneID: 2810
• NCBI Accession #: NP_006133.1
• NCBI GenBank Nucleotide #: NM_006142.3
• UniProt Accession #: P31947; Q6FH30; Q6FH51; Q96DH0
• Molecular Weight: 24,336 Da
• NCBI Official Full Name: 14-3-3 protein sigma
• NCBI Official Synonym Full Names: stratifin
• NCBI Official Symbol: SFN
• NCBI Official Synonym Symbols: YWHAS
• NCBI Protein Information: 14-3-3 protein sigma
• UniProt Protein Name: 14-3-3 protein sigma
• UniProt Gene Name: SFN
• UniProt Synonym Gene Names: HME1
• UniProt Entry Name: 1433S_HUMAN