WB (Western Blot)
(Detection of human SEMA4D by western blot. Samples: Whole cell lysate (50 ug) prepared using NETN or RIPA buffer from HeLa, HEK293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-SEMA4D antibody AAA212666 (lot AAA212666-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Rabbit anti-Human SEMA4D Polyclonal Antibody | anti-SEMA4D antibody
Rabbit anti-SEMA4D Antibody, Affinity Purified
Gene Names
SEMA4D; CD100; SEMAJ; coll-4; C9orf164; M-sema-G
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
SEMA4D, Antibody; Rabbit anti-SEMA4D Antibody, Affinity Purified; C9orf164; semaphorin-4D; SEMAJ; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, 4D; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D; M-sema-G; GR3; coll-4; COLL4; CD antigen CD100; BB18; A8; CD100; Semaphorin-4D; semaphorin 4D; anti-SEMA4D antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-SEMA4D antibody
IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Mouse
Immunogen
Between 812 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human SEMA4D by western blot. Samples: Whole cell lysate (50 ug) prepared using NETN or RIPA buffer from HeLa, HEK293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-SEMA4D antibody AAA212666 (lot AAA212666-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of human SEMA4D by western blot. Samples: Whole cell lysate (50 ug) prepared using NETN or RIPA buffer from HeLa, HEK293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-SEMA4D antibody AAA212666 (lot AAA212666-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human SEMA4D by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using RIPA buffer from Jurkat cells. Antibodies: Affinity purified rabbit anti-SEMA4D antibody AAA212666 (lot AAA212666-1) used for IP at 6 ug per reaction. SEMA4D was also immunoprecipitated by rabbit anti-SEMA4D antibody For blotting immunoprecipitated SEMA4D, AAA212666 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human SEMA4D by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using RIPA buffer from Jurkat cells. Antibodies: Affinity purified rabbit anti-SEMA4D antibody AAA212666 (lot AAA212666-1) used for IP at 6 ug per reaction. SEMA4D was also immunoprecipitated by rabbit anti-SEMA4D antibody For blotting immunoprecipitated SEMA4D, AAA212666 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Related Product Information for anti-SEMA4D antibody
Sema4D (Semaphorin-4D) is a cell surface receptor for PLXN1B and PLXNB2 that plays an important role in cell-cell signaling. Sema4D promotes reorganization of the actin cytoskeleton and plays a role in axonal growth cone guidance in the developing central nervous system. Sema4D regulates dendrite and axon branching and morphogenesis. It promotes the migration of cerebellar granule cells and of endothelial cells. In the immune system Sema4D induces B-cells to aggregate and improves their viability (in vitro). Sema4D also promotes signaling via SRC and PTK2B/PYK2, which then mediates activation of phosphatidylinositol 3-kinase and of the AKT1 signaling cascade [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q92854].
Product Categories/Family for anti-SEMA4D antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
96,150 Da
NCBI Official Full Name
semaphorin-4D isoform 1
NCBI Official Synonym Full Names
sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D
NCBI Official Symbol
SEMA4D
NCBI Official Synonym Symbols
CD100; SEMAJ; coll-4; C9orf164; M-sema-G
NCBI Protein Information
semaphorin-4D; A8; GR3; BB18; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, 4D
UniProt Protein Name
Semaphorin-4D
UniProt Gene Name
SEMA4D
UniProt Synonym Gene Names
C9orf164; CD100; SEMAJ
UniProt Entry Name
SEM4D_HUMAN
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Product Notes
The SEMA4D sema4d (Catalog #AAA212666) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-SEMA4D Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SEMA4D can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the SEMA4D sema4d for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SEMA4D, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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