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FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of WiDr cells using SCF (KITLG) Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit SCF (KITLG) Polyclonal Antibody | anti-KITLG antibody
SCF (KITLG) Antibody (C-term)
Gene Names
KITLG; SF; MGF; SCF; FPH2; FPHH; KL-1; Kitl; SHEP7
Reactivity
Human (Predicted Reactivity: Bovine, Pig)
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, ELISA, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
SCF (KITLG), Antibody; SCF (KITLG) Antibody (C-term); Kit ligand; Mast cell growth factor; MGF; Stem cell factor; SCF; c-Kit ligand; Soluble KIT ligand; sKITLG; KITLG; anti-KITLG antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Bovine, Pig)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This SCF (KITLG) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 244-273 amino acids from the C-terminal region of human SCF (KITLG).
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Positions
244-273
Sequence Length
238
Applicable Applications for anti-KITLG antibody
FCM/FACS (Flow Cytometry), IF (Immunofluorescence), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of WiDr cells using SCF (KITLG) Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of WiDr cells using SCF (KITLG) Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IF (Immunofluorescence)
(Immunofluorescence analysis of anti-KITLG Antibody (C-term) in HeLa cells. 0.025 mg/ml primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L). Alexa-Fluor-546 emits orange fluorescence. Blue counterstaining is DAPI.)
IF (Immunofluorescence)
(Immunofluorescence analysis of anti-KITLG Antibody (C-term) in HeLa cells. 0.025 mg/ml primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L). Alexa-Fluor-546 emits orange fluorescence. Blue counterstaining is DAPI.)
WB (Western Blot)
(Western blot analysis of KITLG(arrow) using rabbit polyclonal KITLG Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the KITLG gene (Lane 2) (Origene Technologies).)
WB (Western Blot)
(Western blot analysis of KITLG(arrow) using rabbit polyclonal KITLG Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the KITLG gene (Lane 2) (Origene Technologies).)
WB (Western Blot)
(Western blot analysis of KITLG Antibody (C-term) in 293 cell line lysates (35ug/lane). KITLG (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of KITLG Antibody (C-term) in 293 cell line lysates (35ug/lane). KITLG (arrow) was detected using the purified Pab.)
Related Product Information for anti-KITLG antibody
KITLG is the ligand of the tyrosine-kinase receptor encoded by the KIT locus. This ligand is a pleiotropic factor that acts in utero in germ cell and neural cell development, and hematopoiesis, all believed to reflect a role in cell migration. In adults, it functions pleiotropically, while mostly noted for its continued requirement in hematopoiesis.
Product Categories/Family for anti-KITLG antibody
References
Young,S.M., Cell. Signal. 19 (12), 2572-2581 (2007)
Pick,M., Stem Cells 25 (9), 2206-2214 (2007)
Yasuda,A., Dig. Dis. Sci. 52 (9), 2292-2300 (2007)
Pick,M., Stem Cells 25 (9), 2206-2214 (2007)
Yasuda,A., Dig. Dis. Sci. 52 (9), 2292-2300 (2007)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
30899
NCBI Official Full Name
kit ligand isoform b
NCBI Official Synonym Full Names
KIT ligand
NCBI Official Symbol
KITLG
NCBI Official Synonym Symbols
SF; MGF; SCF; FPH2; FPHH; KL-1; Kitl; SHEP7
NCBI Protein Information
kit ligand
UniProt Protein Name
Kit ligand
UniProt Gene Name
KITLG
UniProt Synonym Gene Names
MGF; SCF; MGF; SCF; sKITLG
UniProt Entry Name
SCF_HUMAN
Similar Products
Product Notes
The KITLG kitlg (Catalog #AAA284746) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 244-273. The SCF (KITLG) Antibody (C-term) reacts with Human (Predicted Reactivity: Bovine, Pig) and may cross-react with other species as described in the data sheet. AAA Biotech's SCF (KITLG) can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IF (Immunofluorescence), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the KITLG kitlg for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SCF (KITLG), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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