WB (Western Blot)
(Detection of human SARDH by western blot. Samples: Whole cell lysate (50 ug) from Hep-G2, K-562, and LNCaP cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SARDH antibody (AAA213456 lot 1) used for WB at 0.04 mg/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
Rabbit anti-Human SARDH Polyclonal Antibody | anti-SARDH antibody
Rabbit anti-SARDH Antibody, Affinity Purified
Gene Names
SARDH; SAR; SDH; SARD; BPR-2; DMGDHL1
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
SARDH, Antibody; Rabbit anti-SARDH Antibody, Affinity Purified; BPR-2; SarDH; SARD; sarcosine dehydrogenase, mitochondrial; SAR; dimethylglycine dehydrogenase-like 1; SDH; DMGDHL1; Sarcosine dehydrogenase, mitochondrial; sarcosine dehydrogenase; anti-SARDH antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-SARDH antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 225 and 275
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human SARDH by western blot. Samples: Whole cell lysate (50 ug) from Hep-G2, K-562, and LNCaP cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SARDH antibody (AAA213456 lot 1) used for WB at 0.04 mg/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
WB (Western Blot)
(Detection of human SARDH by western blot. Samples: Whole cell lysate (50 ug) from Hep-G2, K-562, and LNCaP cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SARDH antibody (AAA213456 lot 1) used for WB at 0.04 mg/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
IP (Immunoprecipitation)
(Detection of human SARDH by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Hep-G2 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SARDH antibody (AAA213456 lot 1) used for IP at 1 ug per reaction. SARDH was also immunoprecipitated by rabbit anti-SARDH antibody For blotting immunoprecipitated SARDH, AAA213456 was used at 0.04 mg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human SARDH by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Hep-G2 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SARDH antibody (AAA213456 lot 1) used for IP at 1 ug per reaction. SARDH was also immunoprecipitated by rabbit anti-SARDH antibody For blotting immunoprecipitated SARDH, AAA213456 was used at 0.04 mg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Related Product Information for anti-SARDH antibody
SARDH is an enzyme localized to the mitochondrial matrix which catalyzes the oxidative demethylation of sarcosine. This enzyme is distinct from another mitochondrial matrix enzyme, dimethylglycine dehydrogenase, which catalyzes a reaction resulting in the formation of sarcosine. Mutations in this gene are associated with sarcosinemia. Alternatively spliced transcript variants have been described. [taken from NCBI Entrez Gene (Gene ID: 1757)].
Product Categories/Family for anti-SARDH antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
101,037 Da
NCBI Official Full Name
sarcosine dehydrogenase, mitochondrial
NCBI Official Synonym Full Names
sarcosine dehydrogenase
NCBI Official Symbol
SARDH
NCBI Official Synonym Symbols
SAR; SDH; SARD; BPR-2; DMGDHL1
NCBI Protein Information
sarcosine dehydrogenase, mitochondrial; dimethylglycine dehydrogenase-like 1
UniProt Protein Name
Sarcosine dehydrogenase, mitochondrial
UniProt Gene Name
SARDH
UniProt Synonym Gene Names
DMGDHL1; SarDH
UniProt Entry Name
SARDH_HUMAN
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Product Notes
The SARDH sardh (Catalog #AAA213456) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-SARDH Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SARDH can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the SARDH sardh for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SARDH, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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