Rabbit RUVBL1 (TIP49) Polyclonal Antibody | anti-RUVBL1 antibody

RUVBL1 (TIP49) Antibody (Center)

Cat. Num. AAA287661

• Gene Names: RUVBL1; RVB1; TIH1; ECP54; TIP49; INO80H; NMP238; PONTIN; TIP49A; Pontin52
• Reactivity: Human (Predicted Reactivity: Zebrafish, Mouse, Rat)
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: RUVBL1 (TIP49), Antibody; RUVBL1 (TIP49) Antibody (Center); RuvB-like 1; 49 kDa TATA box-binding protein-interacting protein; 49 kDa TBP-interacting protein; 54 kDa erythrocyte cytosolic protein; ECP-54; INO80 complex subunit H; Nuclear matrix protein 238; NMP 238; Pontin 52; TIP49a; TIP60-associated protein 54-alpha; TAP54-alpha; RUVBL1; INO80H; NMP238; TIP49; TIP49A; anti-RUVBL1 antibody

• Host: Rabbit
• Reactivity: Human (Predicted Reactivity: Zebrafish, Mouse, Rat)
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This RUVBL1 (TIP49) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 247-280 amino acids from the Central region of human RUVBL1 (TIP49).
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Concentration: Vial Concentration: 0.35 (varies by lot)
• Sequence Positions: 247-280
• Sequence Length: 386

• Applicable Applications for anti-RUVBL1 antibody: FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

FCM/FACS (Flow Cytometry)

(RUVBL1 (TIP49) Antibody (Center) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

IHC (Immunohiostchemistry)

(RUVBL1 (TIP49) Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RUVBL1 (TIP49) Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

WB (Western Blot)

(Western blot analysis of RUVBL1 (arrow) using rabbit polyclonal RUVBL1 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the RUVBL1 gene.)

Related Product Information for anti-RUVBL1 antibody

RUVBL1 possesses single-stranded DNA-stimulated ATPase and ATP-dependent DNA helicase (3' to 5') activity. It is a component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histone H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. RUVBL1 plays an essential role in oncogenic transformation by MYC and also modulates transcriptional activation by the LEF1/TCF1 -CTNNB1 complex. High levels of autoantibodies against RUVBL1 are detected in sera of patients with autoimmune diseases such as polymyositis/dermatomyosistis and autoimmune hepatitis.

References

Feng, Y., et al., Cancer Res. 63(24):8726-8734 (2003).
Hawley, S.B., et al., J. Biol. Chem. 276(1):179-186 (2001).
Ikura, T., et al., Cell 102(4):463-473 (2000).
Salzer, U., et al., Biochim. Biophys. Acta 1446(3):365-370 (1999).
Makino, Y., et al., Biochem. Biophys. Res. Commun. 245(3):819-823 (1998).

NCBI and Uniprot Product Information

• NCBI GI #: 4506753
• NCBI GeneID: 8607
• NCBI Accession #: NP_003698.1
• NCBI GenBank Nucleotide #: NM_003707.2
• UniProt Accession #: Q9Y265; P82276; Q1KMR0; Q53HK5; Q53HL7; Q53Y27; Q9BSX9; B2R5S0
• Molecular Weight: 50228
• NCBI Official Full Name: ruvB-like 1
• NCBI Official Synonym Full Names: RuvB-like AAA ATPase 1
• NCBI Official Symbol: RUVBL1
• NCBI Official Synonym Symbols: RVB1; TIH1; ECP54; TIP49; INO80H; NMP238; PONTIN; TIP49A; Pontin52
• NCBI Protein Information: ruvB-like 1
• UniProt Protein Name: RuvB-like 1
• UniProt Gene Name: RUVBL1
• UniProt Synonym Gene Names: INO80H; NMP238; TIP49; TIP49A; 49 kDa TBP-interacting protein; ECP-54; NMP 238; TAP54-alpha
• UniProt Entry Name: RUVB1_HUMAN