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product-image-AAA162225_FCM10.jpg FCM/FACS (Flow Cytometry) (Mouse Raf1 Antibody flow cytometry of Neuro-2a cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)

Rabbit anti-Mouse, Human RAF1 / RAF Polyclonal Antibody | anti-RAF1 / RAF antibody

Anti-RAF1 / RAF Antibody (aa85-113) IHC-plus

Gene Names
RAF1; NS5; CRAF; Raf-1; c-Raf; CMD1NN
Reactivity
Mouse, Human
Applications
Flow Cytometry, Functional Assay, Western Blot, Immunohistochemistry
Purity
Immunoaffinity purified
Synonyms
RAF1 / RAF, Antibody; Anti-RAF1 / RAF Antibody (aa85-113) IHC-plus; Rabbit Polyclonal to Mouse RAF1 / RAF; Mouse RAF1 / RAF; RAF1; C-Raf; C-raf-1; CRAF; NS5; Raf-1; Proto-oncogene c-RAF; Oncogene RAF1; RAF; Raf1 kinase; anti-RAF1 / RAF antibody
Ordering
Host
Rabbit
Reactivity
Mouse, Human
Clonality
Polyclonal
Specificity
Mouse RAF
Purity/Purification
Immunoaffinity purified
Form/Format
PBS, 0.09% sodium azide
Sequence Length
648
Applicable Applications for anti-RAF1 / RAF antibody
FCM/FACS (Flow Cytometry), WB (Western Blot), IHC (Immunohistochemistry)
Immunogen
RAF1 / RAF antibody was raised against kLH-conjugated synthetic peptide from N-terminal region of Mouse Raf1.
Immunogen Description
KLH-conjugated synthetic peptide from N-terminal region of Mouse Raf1.
Immunogen Type
Synthetic peptide
Antigen Modification
aa85-113
Target Species
Mouse
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months.
For long term storage store at -20 degree C.

FCM/FACS (Flow Cytometry)

(Mouse Raf1 Antibody flow cytometry of Neuro-2a cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)

product-image-AAA162225_FCM10.jpg FCM/FACS (Flow Cytometry) (Mouse Raf1 Antibody flow cytometry of Neuro-2a cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)

WB (Western Blot)

(Mouse Raf1 Antibody western blot of Jurkat cell line lysates (35 ug/lane). The Raf1 antibody detected the Raf1 protein (arrow).)

product-image-AAA162225_WB11.jpg WB (Western Blot) (Mouse Raf1 Antibody western blot of Jurkat cell line lysates (35 ug/lane). The Raf1 antibody detected the Raf1 protein (arrow).)

WB (Western Blot)

(Mouse Raf1 Antibody western blot of mouse Neuro-2a cell line lysates (35 ug/lane). The Raf1 antibody detected the Raf1 protein (arrow).)

product-image-AAA162225_WB13.jpg WB (Western Blot) (Mouse Raf1 Antibody western blot of mouse Neuro-2a cell line lysates (35 ug/lane). The Raf1 antibody detected the Raf1 protein (arrow).)

IHC (Immunohistochemistry)

(Human Skin: Formalin-Fixed, Paraffin-Embedded (FFPE))

product-image-AAA162225_IHC15.jpg IHC (Immunohistochemistry) (Human Skin: Formalin-Fixed, Paraffin-Embedded (FFPE))
Related Product Information for anti-RAF1 / RAF antibody
RAF1 Antibody, C-Raf Antibody, C-raf-1 Antibody, CRAF Antibody, NS5 Antibody, Raf-1 Antibody, Proto-oncogene c-RAF Antibody, Oncogene RAF1 Antibody, RAF Antibody, Raf1 kinase Antibody Description: Serine/threonine-protein kinase that acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions including proliferation, differentiation, apoptosis, survival and oncogenic transformation.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
75,395 Da
NCBI Official Full Name
RAF proto-oncogene serine/threonine-protein kinase
NCBI Official Synonym Full Names
Raf-1 proto-oncogene, serine/threonine kinase
NCBI Official Symbol
RAF1
NCBI Official Synonym Symbols
NS5; CRAF; Raf-1; c-Raf; CMD1NN
NCBI Protein Information
RAF proto-oncogene serine/threonine-protein kinase
UniProt Protein Name
RAF proto-oncogene serine/threonine-protein kinase
UniProt Gene Name
RAF1
UniProt Synonym Gene Names
RAF; cRaf
UniProt Entry Name
RAF1_HUMAN

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Product Notes

The RAF1 / RAF raf1 (Catalog #AAA162225) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-RAF1 / RAF Antibody (aa85-113) IHC-plus reacts with Mouse, Human and may cross-react with other species as described in the data sheet. AAA Biotech's RAF1 / RAF can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), WB (Western Blot), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the RAF1 / RAF raf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RAF1 / RAF, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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