WB (Western Blot)
(Western Blot analysis of A549 cells using Phospho-Raf-1 (S301) Polyclonal Antibody diluted at 1:1000)
Rabbit Raf-1 Polyclonal Antibody | anti-Raf-1 antibody
Raf-1 (Phospho-Ser301) Rabbit Polyclonal Antibody
Gene Names
RAF1; NS5; CRAF; Raf-1; c-Raf
Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
Raf-1, Antibody; Raf-1 (Phospho-Ser301) Rabbit Polyclonal Antibody; RAF1; RAF; RAF proto-oncogene serine/threonine-protein kinase; Proto-oncogene c-RAF; cRaf; Raf-1; BH6842; anti-Raf-1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
Phospho-Raf-1 (S301) Polyclonal Antibody detects endogenous levels of Raf-1 protein only when phosphorylated at S301.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-Raf-1 antibody
ELISA, WB (Western Blot)
Immunogen
Synthesized phospho-peptide around the phosphorylation site of human Raf-1 (phospho Ser301)
Modification
Phospho Specific
Preparation and Storage
Store at -20 degree C/1 year
WB (Western Blot)
(Western Blot analysis of A549 cells using Phospho-Raf-1 (S301) Polyclonal Antibody diluted at 1:1000)
WB (Western Blot)
(Western Blot analysis of A549 cells using Phospho-Raf-1 (S301) Polyclonal Antibody diluted at 1:1000)
Application Data
(The picture was kindly provided by our customer,antibody was diluted at 1:500)
Application Data
(The picture was kindly provided by our customer,antibody was diluted at 1:500)
Related Product Information for anti-Raf-1 antibody
This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2. [provided by RefSeq, Jul 2008]
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
73,052 Da
NCBI Official Full Name
RAF proto-oncogene serine/threonine-protein kinase
NCBI Official Synonym Full Names
v-raf-1 murine leukemia viral oncogene homolog 1
NCBI Official Symbol
RAF1
NCBI Official Synonym Symbols
NS5; CRAF; Raf-1; c-Raf
NCBI Protein Information
RAF proto-oncogene serine/threonine-protein kinase; Oncogene RAF1; proto-oncogene c-RAF; raf proto-oncogene serine/threonine protein kinase
UniProt Protein Name
RAF proto-oncogene serine/threonine-protein kinase
UniProt Gene Name
RAF1
UniProt Synonym Gene Names
RAF; cRaf
UniProt Entry Name
RAF1_HUMAN
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Product Notes
The Raf-1 raf1 (Catalog #AAA318651) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Raf-1 (Phospho-Ser301) Rabbit Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Raf-1 can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the Raf-1 raf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Raf-1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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