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IHC (Immunohistochemistry)
(Immunohistochemical analysis of AAA284335 on paraffin-embedded Human kidney tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.)
Rabbit Prohibitin (PHB1) Polyclonal Antibody | anti-PHB antibody
Prohibitin (PHB1) Antibody (N-term)
Gene Names
PHB; PHB1; HEL-215; HEL-S-54e
Reactivity
Human, MousePredicted Reactivity: Bovine, Chicken
Applications
Immunohistochemistry, Western Blot, ELISA, Flow Cytometry, Functional Assay
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
Prohibitin (PHB1), Antibody; Prohibitin (PHB1) Antibody (N-term); Prohibitin; PHB; anti-PHB antibody
Host
Rabbit
Reactivity
Human, Mouse
Predicted Reactivity: Bovine, Chicken
Predicted Reactivity: Bovine, Chicken
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This Prohibitin (PHB1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 92-121 amino acids from the N-terminal region of human Prohibitin (PHB1).
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Positions
92-121
Sequence Length
155
Applicable Applications for anti-PHB antibody
IHC (Immunohistochemistry), WB (Western Blot), ELISA, FCM/FACS (Flow Cytometry)
Antigen Type
Synthetic Peptide
Cellular Location
Mitochondrion inner membrane. Nucleus. Cytoplasm. Cell membrane
Tissue Location
Widely expressed in different tissues.
Antigen Source
HUMAN
Function
Protein with pleiotropic attributes mediated in a cell- compartment- and tissue-specific manner, which include the plasma membrane-associated cell signaling functions, mitochondrial chaperone, and transcriptional co-regulator of transcription factors in the nucleus (PubMed: , PubMed: , PubMed: , PubMed: ). Plays a role in adipose tissue and glucose Homeostasis in a sex-specific manner (By similarity). Contributes to pulmonary vascular remodeling by accelerating proliferation of pulmonary arterial smooth muscle cells (By similarity).
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks.
For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohistochemistry)
(Immunohistochemical analysis of AAA284335 on paraffin-embedded Human kidney tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of AAA284335 on paraffin-embedded Human kidney tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.)
IHC (Immunohistochemisry)
(Immunohistochemical analysis of AAA284335 on paraffin-embedded Human liver tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody (1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.)
IHC (Immunohistochemisry)
(Immunohistochemical analysis of AAA284335 on paraffin-embedded Human liver tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody (1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of ATDC5 cells using PHB1 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of ATDC5 cells using PHB1 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
WB (Western Blot)
(All lanes : Anti-Prohibitin (PHB1) Antibody (N-term) at 1:2000 dilution Lane 1: MCF-7 whole cell lysate Lane 2: HepG2 whole celllysate Lane 3: A431 whole cell lysate Lane 4: Jurkat whole cell lysate Lane 5: Hela whole cell lysate Lane 6: Mouse kidney tissue lysate Lane 7: NIH/3T3 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 30 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(All lanes : Anti-Prohibitin (PHB1) Antibody (N-term) at 1:2000 dilution Lane 1: MCF-7 whole cell lysate Lane 2: HepG2 whole celllysate Lane 3: A431 whole cell lysate Lane 4: Jurkat whole cell lysate Lane 5: Hela whole cell lysate Lane 6: Mouse kidney tissue lysate Lane 7: NIH/3T3 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 30 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-PHB antibody
Prohibitin is an evolutionarily conserved protein that is ubiquitously expressed. It is thought to be a negative regulator of cell proliferation and may be a tumor suppressor. Mutations have been linked to sporadic breast cancer. Prohibitin is expressed as two transcripts with varying lengths of 3' untranslated region.
Product Categories/Family for anti-PHB antibody
References
Gregory-Bass,R.C., Int. J. Cancer 122 (9), 1923-1930 (2008) Ross,J.A., J. Biol. Chem. 283 (8), 4699-4713 (2008) White,J.J., Genomics 11 (1), 228-230 (1991)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
29804
NCBI Official Full Name
prohibitin isoform 1
NCBI Official Synonym Full Names
prohibitin
NCBI Official Symbol
PHB
NCBI Official Synonym Symbols
PHB1; HEL-215; HEL-S-54e
NCBI Protein Information
prohibitin
UniProt Protein Name
Prohibitin
UniProt Gene Name
PHB
UniProt Entry Name
PHB_HUMAN
Similar Products
Product Notes
The PHB phb (Catalog #AAA284335) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 92-121. The Prohibitin (PHB1) Antibody (N-term) reacts with Human, Mouse Predicted Reactivity: Bovine, Chicken and may cross-react with other species as described in the data sheet. AAA Biotech's Prohibitin (PHB1) can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot), ELISA, FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the PHB phb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Prohibitin (PHB1), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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