Rabbit PRKAR1A Polyclonal Antibody | anti-PRKAR1A antibody

PRKAR1A Antibody

Cat. Num. AAA306543

• Gene Names: PRKAR1A; CAR; CNC; CNC1; PKR1; TSE1; ADOHR; PPNAD1; PRKAR1; ACRDYS1
• Reactivity: Human, Mouse, Rat
• Applications: Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: Antibodies were purified by affinity purification using immunogen.
• Synonyms: PRKAR1A, Antibody; PRKAR1A Antibody; PRKAR1A; CAR; CNC; CNC1; DKFZp779L0468; anti-PRKAR1A antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total PRKAR1A protein.
• Purity/Purification: Antibodies were purified by affinity purification using immunogen.
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 381

• Applicable Applications for anti-PRKAR1A antibody: IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
• Immunogen Type: Recombinant Protein
• Immunogen Description: Recombinant protein of human PRKAR1A.
• Target Name: PRKAR1A
• Preparation and Storage: Store at -20 degree C

IF (Immunofluorescence)

(Immunofluorescence analysis of HeLa cell using PRKAR1A antibody. Blue: DAPI for nuclear staining.)

IHC (Immunohistochemisry)

(Immunohistochemical analysis of paraffin-embedded human esophagus using PRKAR1A antibody at dilution of 1:100 (200x lens).)

IHC (Immunohiostchemistry)

(Immunohistochemical analysis of paraffin-embedded human esophageal cancer using PRKAR1A antibody at dilution of 1:100 (200x lens).)

WB (Western Blot)

(Western blot analysis of extracts of various cell lines using PRKAR1A antibody.)

Related Product Information for anti-PRKAR1A antibody

The second messenger cyclic AMP (cAMP) activates cAMP-dependent protein kinase (PKA or cAPK) in mammalian cells and controls many cellular mechanisms such as gene transcription, ion transport, and protein phosphorylation (1). Inactive PKA is a heterotetramer composed of a regulatory subunit (R) dimer and a catalytic subunit (C) dimer. In this inactive state, the pseudosubstrate sequences on the R subunits block the active sites on the C subunits. Three C subunit isoforms (C-a, C-b, and C-g) and two families of regulatory subunits (RI and RII) with distinct cAMP binding properties have been identified. The two R families exist in two isoforms, a and b (RI-a, RI-b, RII-a, and RII-b). Upon binding of cAMP to the R subunits, the autoinhibitory contact is eased and active monomeric C subunits are released. PKA shares substrate specificity with Akt (PKB) and PKC, which are characterized by an arginine at position -3 relative to the phosphorylated serine or threonine residue (2). Substrates that present this consensus sequence and have been shown to be phosphorylated by PKA are Bad (Ser155), CREB (Ser133), and GSK-3 (GSK-3a Ser21 and GSK-3b Ser9) (3-5). In addition, combined knock-down of PKA C-a and -b blocks cAMP-mediated phosphorylation of Raf (Ser43 and Ser259) (6). Autophosphorylation and phosphorylation by PDK-1 are two known mechanisms responsible for phosphorylation of the C subunit at Thr197 (7).

Product Categories/Family for anti-PRKAR1A antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 443497964
• NCBI GeneID: 5573
• NCBI Accession #: NP_001263218.1
• NCBI GenBank Nucleotide #: NM_001276289.1
• UniProt Accession #: P10644; Q567S7; K7ER48
• Molecular Weight: 38,003 Da
• NCBI Official Full Name: cAMP-dependent protein kinase type I-alpha regulatory subunit isoform a
• NCBI Official Synonym Full Names: protein kinase, cAMP-dependent, regulatory, type I, alpha
• NCBI Official Symbol: PRKAR1A
• NCBI Official Synonym Symbols: CAR; CNC; CNC1; PKR1; TSE1; ADOHR; PPNAD1; PRKAR1; ACRDYS1
• NCBI Protein Information: cAMP-dependent protein kinase type I-alpha regulatory subunit
• UniProt Protein Name: cAMP-dependent protein kinase type I-alpha regulatory subunit
• UniProt Gene Name: PRKAR1A
• UniProt Synonym Gene Names: PKR1; PRKAR1; TSE1; TSE1
• UniProt Entry Name: KAP0_HUMAN