WB (Western Blot)
(Detection of human PPP2R1A by western blot. Samples: Whole cell lysate (25 ug) from RKO, HEK293T, Jurkat, MCF-7, and U2OS cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PPP2R1A antibody (AAA211251 lot 3) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
Rabbit anti-Human, Mouse PPP2R1A Polyclonal Antibody | anti-PPP2R1A antibody
Rabbit anti-PPP2R1A Antibody, Affinity Purified
Gene Names
PPP2R1A; MRD36; PR65A; PP2AAALPHA; PP2A-Aalpha
Reactivity
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
PPP2R1A, Antibody; Rabbit anti-PPP2R1A Antibody, Affinity Purified; serine/threonine protein phosphatase 2A, 65 kDa regulatory subunit A, alpha isoform; serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform; protein phosphatase 2A structural subunit A, alpha isoform; protein phosphatase 2, regulatory subunit A, alpha; protein phosphatase 2 (formerly 2A), regulatory subunit A (PR 65), alpha isoform; PR65A; PP2AAALPHA; PP2AA; PP2A subunit A isoform R1-alpha; PP2A subunit A isoform PR65-alpha; MRD36; medium tumor antigen-associated 61 KDA protein; PP2A-Aalpha; testicular secretory protein Li 1; Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform; protein phosphatase 2 scaffold subunit Aalpha; anti-PPP2R1A antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-PPP2R1A antibody
IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Bovine, Pig
Immunogen
Between 539 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human PPP2R1A by western blot. Samples: Whole cell lysate (25 ug) from RKO, HEK293T, Jurkat, MCF-7, and U2OS cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PPP2R1A antibody (AAA211251 lot 3) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
WB (Western Blot)
(Detection of human PPP2R1A by western blot. Samples: Whole cell lysate (25 ug) from RKO, HEK293T, Jurkat, MCF-7, and U2OS cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PPP2R1A antibody (AAA211251 lot 3) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
WB (Western Blot)
(Detection of mouse PPP2R1A by western blot. Samples: Whole cell lysate (25 ug) from BW5147.3, CT26, TCMK-1, NIH 3T3, and CH27 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PPP2R1A antibody (AAA211251 lot 3) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
WB (Western Blot)
(Detection of mouse PPP2R1A by western blot. Samples: Whole cell lysate (25 ug) from BW5147.3, CT26, TCMK-1, NIH 3T3, and CH27 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PPP2R1A antibody (AAA211251 lot 3) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human PPP2R1A by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PPP2R1A antibody (AAA211251-3 lot 1) used for IP at 6 ug per reaction. PPP2R1A was also immunoprecipitated by PPP2R1A . For blotting immunoprecipitated PPP2R1A, AAA211251 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
IP (Immunoprecipitation)
(Detection of human PPP2R1A by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PPP2R1A antibody (AAA211251-3 lot 1) used for IP at 6 ug per reaction. PPP2R1A was also immunoprecipitated by PPP2R1A . For blotting immunoprecipitated PPP2R1A, AAA211251 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
Related Product Information for anti-PPP2R1A antibody
PP2A is a major serine/threonine phosphatase that is implicated in the negative control of cell cycle progression. It is a trimeric holoenzyme that is comprised of a catalytic, structural, and regulatory subunit. PPP2R1A is the alpha isoform of the 65kd structural subunit of PP2A. The PP2A catalytic subunit complexed with PPP2R1A can associate with various regulatory subunits to form trimeric holoenzymes with distinct activities.
Product Categories/Family for anti-PPP2R1A antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
65,309 Da
NCBI Official Full Name
serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform
NCBI Official Synonym Full Names
protein phosphatase 2 regulatory subunit A, alpha
NCBI Official Symbol
PPP2R1A
NCBI Official Synonym Symbols
MRD36; PR65A; PP2AAALPHA; PP2A-Aalpha
NCBI Protein Information
serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform
UniProt Protein Name
Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform
UniProt Gene Name
PPP2R1A
UniProt Entry Name
2AAA_HUMAN
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Product Notes
The PPP2R1A ppp2r1a (Catalog #AAA211251) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-PPP2R1A Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PPP2R1A can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the PPP2R1A ppp2r1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PPP2R1A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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