WB (Western Blot)
(Anti-POLK Antibody - Western Blot. Western blot of Affinity Purified anti-POLK antibody shows detection of a band ~98 kD corresponding to human POLK (arrowhead). Approximately 35 ug of a HeLa whole cell lysate was separated by 4-20% SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1000. Reaction occurred overnight at 4? followed by washes and reaction with a 1:20000 dilution of IRDye800 conjugated Rb-a-Goat IgG [H&L] MXHu (for 45 min at room temperature. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.)
Goat anti-Human POLK Polyclonal Antibody | anti-POLK antibody
Goat Polyclonal to Human POLK
Gene Names
POLK; DINP; POLQ; DINB1
Reactivity
Human
Applications
ELISA, Western Blot, Immunofluorescence, Immunohistochemistry
Purity
Immunoaffinity Purified
Synonyms
POLK, Antibody; Goat Polyclonal to Human POLK; Anti-POLK Antibody (aa817-830) IHC-plus; POLK; DINP; DNA polymerase kappa; DINB1; DINB protein; Human POLK; anti-POLK antibody
Host
Goat
Reactivity
Human
Clonality
Polyclonal
Specificity
Amino acids 817-830 of Human POLK (polymerase (DNA directed) kappa).
Purity/Purification
Immunoaffinity Purified
Form/Format
0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Concentration
1 mg/ml (varies by lot)
Sequence Length
870
Applicable Applications for anti-POLK antibody
ELISA, WB (Western Blot), IF (Immunofluorescence), IHC (Immunohistochemistry)
Target Species
Human
Immunogen Description
Synthetic peptide from human POLK.
Immunogen Type
Synthetic peptide
Immunogen
POLK antibody was raised against synthetic peptide from human POLK.
Antigen Modification
aa817-830
Preparation and Storage
Long term: -20 degree C; Short term: +4 degree C; Avoid freeze-thaw cycles.
WB (Western Blot)
(Anti-POLK Antibody - Western Blot. Western blot of Affinity Purified anti-POLK antibody shows detection of a band ~98 kD corresponding to human POLK (arrowhead). Approximately 35 ug of a HeLa whole cell lysate was separated by 4-20% SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1000. Reaction occurred overnight at 4? followed by washes and reaction with a 1:20000 dilution of IRDye800 conjugated Rb-a-Goat IgG [H&L] MXHu (for 45 min at room temperature. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.)
WB (Western Blot)
(Anti-POLK Antibody - Western Blot. Western blot of Affinity Purified anti-POLK antibody shows detection of a band ~98 kD corresponding to human POLK (arrowhead). Approximately 35 ug of a HeLa whole cell lysate was separated by 4-20% SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1000. Reaction occurred overnight at 4? followed by washes and reaction with a 1:20000 dilution of IRDye800 conjugated Rb-a-Goat IgG [H&L] MXHu (for 45 min at room temperature. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.)
IHC (Immunohistochemistry)
(Anti-POLK antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
IHC (Immunohistochemistry)
(Anti-POLK antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
88,342 Da
NCBI Official Full Name
DNA polymerase kappa
NCBI Official Synonym Full Names
polymerase (DNA directed) kappa
NCBI Official Symbol
POLK
NCBI Official Synonym Symbols
DINP; POLQ; DINB1
NCBI Protein Information
DNA polymerase kappa
UniProt Protein Name
DNA polymerase kappa
UniProt Gene Name
POLK
UniProt Synonym Gene Names
DINB1; DINP
UniProt Entry Name
POLK_HUMAN
Similar Products
Product Notes
The POLK polk (Catalog #AAA50739) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat Polyclonal to Human POLK reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's POLK can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot), IF (Immunofluorescence), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the POLK polk for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "POLK, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.