WB (Western Blot)
(Detection of human PLK1 by western blot and immunoprecipitation. Samples: Whole cell lysate (50 ug for A; 600 ug for B) from HeLa cells. Antibodies: A. Affinity purified rabbit anti-PLK1 antibody AAA210843 used at the indicated concentrations for WB. B. Affinity purified rabbit anti-PLK1 antibodies AAA210843, and BL978 used at 2 ug/600 ug lysate for IP. Immunoprecipitated PLK1 was detected using BL978 at 0.5 ug/ml. Detection: Chemiluminescence with exposure times of less than 5 minutes.)
Rabbit anti-Human PLK1 Polyclonal Antibody | anti-PLK1 antibody
Rabbit anti-PLK1 Antibody, Affinity Purified
Gene Names
PLK1; PLK; STPK13
Reactivity
Human
Applications
Immunocytochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
PLK1, Antibody; Rabbit anti-PLK1 Antibody, Affinity Purified; PLK; serine/threonine-protein kinase 13; STPK13; serine/threonine-protein kinase PLK1; cell cycle regulated protein kinase; PLK-1; Polo-like kinase 1; polo (Drosophia)-like kinase; Serine/threonine-protein kinase PLK1; polo like kinase 1; anti-PLK1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-PLK1 antibody
ICC (Immunocytochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 325 and 375
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human PLK1 by western blot and immunoprecipitation. Samples: Whole cell lysate (50 ug for A; 600 ug for B) from HeLa cells. Antibodies: A. Affinity purified rabbit anti-PLK1 antibody AAA210843 used at the indicated concentrations for WB. B. Affinity purified rabbit anti-PLK1 antibodies AAA210843, and BL978 used at 2 ug/600 ug lysate for IP. Immunoprecipitated PLK1 was detected using BL978 at 0.5 ug/ml. Detection: Chemiluminescence with exposure times of less than 5 minutes.)
WB (Western Blot)
(Detection of human PLK1 by western blot and immunoprecipitation. Samples: Whole cell lysate (50 ug for A; 600 ug for B) from HeLa cells. Antibodies: A. Affinity purified rabbit anti-PLK1 antibody AAA210843 used at the indicated concentrations for WB. B. Affinity purified rabbit anti-PLK1 antibodies AAA210843, and BL978 used at 2 ug/600 ug lysate for IP. Immunoprecipitated PLK1 was detected using BL978 at 0.5 ug/ml. Detection: Chemiluminescence with exposure times of less than 5 minutes.)
WB (Western Blot)
(Detection of human PLK1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PLK1 antibody AAA210843 (lot AAA210843-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of human PLK1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PLK1 antibody AAA210843 (lot AAA210843-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
ICC (Immunocytochemistry)
(Detection of human PLK1 by immunocytochemistry. Sample: Formaldehyde-fixed asynchronous HeLa cells. Antibody: Affinity purified rabbit anti-PLK1 (Cat. No.AAA210843 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: Green fluorescent goat anti-rabbit IgG highly cross-adsorbed Antibody FITC conjugated (Cat. No. used at a dilution of 1:100.)
ICC (Immunocytochemistry)
(Detection of human PLK1 by immunocytochemistry. Sample: Formaldehyde-fixed asynchronous HeLa cells. Antibody: Affinity purified rabbit anti-PLK1 (Cat. No.AAA210843 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: Green fluorescent goat anti-rabbit IgG highly cross-adsorbed Antibody FITC conjugated (Cat. No. used at a dilution of 1:100.)
Related Product Information for anti-PLK1 antibody
Polo-like kinase 1 (PLK1) activity is central to the progression through mitosis. PLK1 plays an essential role in spindle formation and chromosome segregation. Recent studies suggest that PLK1 is specifically involved in anaphase spindle elongation, Rho localization to the contractile ring and cytokinesis.
Product Categories/Family for anti-PLK1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
68,255 Da
NCBI Official Full Name
serine/threonine-protein kinase PLK1
NCBI Official Synonym Full Names
polo-like kinase 1
NCBI Official Symbol
PLK1
NCBI Official Synonym Symbols
PLK; STPK13
NCBI Protein Information
serine/threonine-protein kinase PLK1; PLK-1; polo like kinase; polo (Drosophia)-like kinase; serine/threonine-protein kinase 13; cell cycle regulated protein kinase
UniProt Protein Name
Serine/threonine-protein kinase PLK1
UniProt Gene Name
PLK1
UniProt Synonym Gene Names
PLK; PLK-1; STPK13
UniProt Entry Name
PLK1_HUMAN
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Product Notes
The PLK1 plk1 (Catalog #AAA210843) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-PLK1 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PLK1 can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the PLK1 plk1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PLK1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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