IF (Immunofluorescence)
(Immunofluorescence staining of PIWIL4 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human PIWIL4 polyclonal antibody (1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm and nucleus.)
Rabbit anti-Human PIWIL4 Polyclonal Antibody | anti-PIWIL4 antibody
Anti-PIWIL4 Antibody, Rabbit Polyclonal
Gene Names
PIWIL4; HIWI2; MIWI2
Reactivity
Human
Applications
Immunofluorescence, Immunocytochemistry, Immunohistochemistry
Purity
Protein A & Antigen Affinity
Synonyms
PIWIL4, Antibody; Anti-PIWIL4 Antibody, Rabbit Polyclonal; PIWIL4 Antibody, Rabbit PAb, Antigen Affinity Purified; piwi-like RNA-mediated gene silencing 4; Anti-HIWI2 Antibody; Anti-MIWI2 Antibody; anti-PIWIL4 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human PIWIL4
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-PIWIL4 antibody
IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry)
Immunogen
A synthetic peptide corresponding to the N-terminus of the Human PIWIL4
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human PIWIL4, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
IF (Immunofluorescence)
(Immunofluorescence staining of PIWIL4 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human PIWIL4 polyclonal antibody (1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm and nucleus.)
IF (Immunofluorescence)
(Immunofluorescence staining of PIWIL4 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human PIWIL4 polyclonal antibody (1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm and nucleus.)
IHC (Immunohistochemistry)
(Immunochemical staining of human PIWIL4 in human testis with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)
IHC (Immunohistochemistry)
(Immunochemical staining of human PIWIL4 in human testis with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)
Related Product Information for anti-PIWIL4 antibody
PIWIL4 has been identified as a functional protein involved in tumorigenesis. PIWIL4 was identified as a target of miR-384 and plays an oncogenic role in glioma. One of the four PIWI proteins in humans, PIWIL4, is highly expressed in both breast cancer tissues and the cytoplasm of MDA-MB-231 cells derived from breast cancer. Reducing PIWIL4 expression drastically impairs the migration ability of MDA-MB-231 cells, significantly increases their apoptosis, and mildly affects their proliferation. PIWIL4 plays important roles in the chromatin-modifying pathway in human somatic cells.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
852
NCBI Official Full Name
Piwi-like protein 4
NCBI Official Synonym Full Names
piwi-like RNA-mediated gene silencing 4
NCBI Official Symbol
PIWIL4
NCBI Official Synonym Symbols
HIWI2; MIWI2
NCBI Protein Information
piwi-like protein 4; piwi-like 4
UniProt Protein Name
Piwi-like protein 4
UniProt Gene Name
PIWIL4
UniProt Synonym Gene Names
HIWI2; PIWI
UniProt Entry Name
PIWL4_HUMAN
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Product Notes
The PIWIL4 piwil4 (Catalog #AAA254623) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-PIWIL4 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PIWIL4 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the PIWIL4 piwil4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PIWIL4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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