Rabbit anti-Human PIGR Polyclonal Antibody | anti-PIGR antibody

PIGR Antibody (C-term)

Cat. Num. AAA287810

• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry, ELISA, Western Blot
• Purity: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Synonyms: PIGR, Antibody; PIGR Antibody (C-term); Polymeric immunoglobulin receptor; PIgR; Poly-Ig receptor; Hepatocellular carcinoma-associated protein TB6; Secretory component; PIGR; anti-PIGR antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: This PIGR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 646-672 amino acids from the C-terminal region of human PIGR.
• Purity/Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Sequence Positions: 646-672
• Sequence Length: 764

• Applicable Applications for anti-PIGR antibody: FCM/FACS (Flow Cytometry), IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
• Function: [Polymeric immunoglobulin receptor]: Mediates selective transcytosis of polymeric IgA and IgM across mucosal epithelial cells. Binds polymeric IgA and IgM at the basolateral surface of epithelial cells. The complex is then transported across the cell to be secreted at the apical surface. During this process, a cleavage occurs that separates the extracellular (known as the secretory component) from the transmembrane segment.
• Cellular Location: [Polymeric immunoglobulin receptor]: Cell membrane; Single-pass type I membrane protein
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

WB (Western Blot)

(Anti-PIGR Antibody (C-term) at 1:1000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 83 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)

FCM/FACS (Flow Cytometry)

(Flow cytometric analysis of HepG2 cells using PIGR Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

IF (Immunofluorescence)

(Confocal immunofluorescent analysis of PIGR Antibody (C-term) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

IHC (Immunohistochemisry)

(PIGR Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PIGR Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

IHC (Immunohiostchemistry)

(Formalin-fixed and paraffin-embedded human hepatocarcinoma with PIGR Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

WB (Western Blot)

(Western blot analysis of PIGR Antibody (C-term) in HepG2(lane 1),K562(lane 2),HL-60(lane 3) cell line lysates (35ug/lane).PIGR (arrow) was detected using the purified Pab.)

Related Product Information for anti-PIGR antibody

PIGR binds polymeric IgA and IgM at the basolateral surface of epithelial cells. The complex is then transported across the cell to be secreted at the apical surface. During this process a cleavage occurs that separates the extracellular (known as the secretory component) from the transmembrane segment.

Product Categories/Family for anti-PIGR antibody

Immunology

References

Ewing,R.M., et.al., Mol. Syst. Biol. 3, 89 (2007)
Orzech,E., Cohen,S., et.al., J. Biol. Chem. 275 (20), 15207-15219 (2000)

NCBI and Uniprot Product Information

• NCBI GI #: 31377806
• NCBI GeneID: 5284
• NCBI Accession #: NP_002635.2
• NCBI GenBank Nucleotide #: NM_002644.3
• UniProt Accession #: P01833; Q68D81; Q8IZY7
• Molecular Weight: 83284
• NCBI Official Full Name: polymeric immunoglobulin receptor
• NCBI Official Synonym Full Names: polymeric immunoglobulin receptor
• NCBI Official Symbol: PIGR
• NCBI Protein Information: polymeric immunoglobulin receptor
• UniProt Protein Name: Polymeric immunoglobulin receptor
• UniProt Gene Name: PIGR
• UniProt Synonym Gene Names: PIgR; Poly-Ig receptor
• UniProt Entry Name: PIGR_HUMAN