WB (Western Blot)
(Detection of human Periplakin/PPL by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibody: Affinity purified rabbit anti-Periplakin/PPL antibody AAA211273 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate (B). Periplakin/PPL was also immunoprecipitated by rabbit anti-Periplakin/PPL antibody BL4370, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A and B).)
Rabbit Periplakin/PPL Polyclonal Antibody | anti-PPL antibody
Rabbit anti-Periplakin/PPL Antibody, Affinity Purified
Reactivity
Human, Mouse, Rat
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
Periplakin/PPL, Antibody; Rabbit anti-Periplakin/PPL Antibody, Affinity Purified; 190 kDa paraneoplastic pemphigus antigen; 195 kDa cornified envelope precursor protein; periplakin; Periplakin; anti-PPL antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-PPL antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 1150 and 1200
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human Periplakin/PPL by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibody: Affinity purified rabbit anti-Periplakin/PPL antibody AAA211273 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate (B). Periplakin/PPL was also immunoprecipitated by rabbit anti-Periplakin/PPL antibody BL4370, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A and B).)
WB (Western Blot)
(Detection of human Periplakin/PPL by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibody: Affinity purified rabbit anti-Periplakin/PPL antibody AAA211273 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate (B). Periplakin/PPL was also immunoprecipitated by rabbit anti-Periplakin/PPL antibody BL4370, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A and B).)
WB (Western Blot)
(Detection of mouse and rat Periplakin/PPL by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells. Antibodies: Affinity purified rabbit anti-Periplakin/PPL antibody AAA211273 (lot AAA211273-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of mouse and rat Periplakin/PPL by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells. Antibodies: Affinity purified rabbit anti-Periplakin/PPL antibody AAA211273 (lot AAA211273-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Related Product Information for anti-PPL antibody
Periplakin is a protein that localizes to the desmosomes and interdesomosomal plasma membrane of keratinocytes in the cornified layer of the epidermis. Periplakin belongs to the plakin family of cytolinker proteins that function as a link between cytoskeletal networks and membrane-associated adhesive sites. Periplakin has been reported to interact with AKT1/PKB and may function in facilitating AKT1/PKB signaling.
Product Categories/Family for anti-PPL antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The PPL ppl (Catalog #AAA211273) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-Periplakin/PPL Antibody, Affinity Purified reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Periplakin/PPL can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the PPL ppl for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Periplakin/PPL, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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