WB (Western Blot)
(Detection of human PDZ-GEF1 by western blot. Samples:Whole cell lysate (50 ug) from HeLa, Hep-G2, Jurkat, and GaMG cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PDZ-GEF1 antibody AAA211700 (lot AAA211700-4) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Rabbit anti-Human PDZ-GEF1 Polyclonal Antibody | anti-PDZ-GEF1 antibody
Rabbit anti-PDZ-GEF1 Antibody, Affinity Purified
Gene Names
RAPGEF2; RA-GEF; NRAPGEP; PDZGEF1; Rap-GEP; CNrasGEF; PDZ-GEF1; RA-GEF-1; nRap GEP
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
PDZ-GEF1, Antibody; Rabbit anti-PDZ-GEF1 Antibody, Affinity Purified; RAGEF; RA-GEF; RA-GEF-1; Rap guanine nucleotide exchange factor (GEF) 2; rap guanine nucleotide exchange factor 2; Rap-GEP; RA(Ras/Rap1A-associating)-GEF; neural RAP guanine nucleotide exchange protein; ras/Rap1-associating GEF-1; PDZGEF1; PDZ domain-containing guanine nucleotide exchange factor 1; PDZ domain containing guanine nucleotide exchange factor (GEF) 1; CNrasGEF; nRap GEP; PDZ-GEF1; cyclic nucleotide ras GEF; NRAPGEP; Rap guanine nucleotide exchange factor 2; anti-PDZ-GEF1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-PDZ-GEF1 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Mouse, Dog
Immunogen
Between 1000 and 1050
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human PDZ-GEF1 by western blot. Samples:Whole cell lysate (50 ug) from HeLa, Hep-G2, Jurkat, and GaMG cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PDZ-GEF1 antibody AAA211700 (lot AAA211700-4) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of human PDZ-GEF1 by western blot. Samples:Whole cell lysate (50 ug) from HeLa, Hep-G2, Jurkat, and GaMG cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PDZ-GEF1 antibody AAA211700 (lot AAA211700-4) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human PDZ-GEF1 by western blot of immunoprecipitates. Samples:Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PDZ-GEF1 antibody AAA211700 (lot AAA211700-4) used for IP at 6 ug per reaction. For blotting immunoprecipitated PDZ-GEF1, AAA211700 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human PDZ-GEF1 by western blot of immunoprecipitates. Samples:Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PDZ-GEF1 antibody AAA211700 (lot AAA211700-4) used for IP at 6 ug per reaction. For blotting immunoprecipitated PDZ-GEF1, AAA211700 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IHC (Immunohistochemistry)
(Detection of human PDZ-GEF1 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-PDZ-GEF1 (Cat. No. AAA211700 lot 4) used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human PDZ-GEF1 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-PDZ-GEF1 (Cat. No. AAA211700 lot 4) used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB)
Related Product Information for anti-PDZ-GEF1 antibody
PDZ-GEF1 is a guanine nucleotide exchange factor (GEF) for Rap GTPases. It contains one PDZ-signaling domain, a Ras-association (RA) domain, and a region related to a cyclic nucleotide binding domain (RCBD). PDZ-GEF1 functions to catalyze the exchange of GDP for GTP and activate Rap1A, Rap1B, and Rap2B GTPase activity.
Product Categories/Family for anti-PDZ-GEF1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
167,417 Da
NCBI Official Full Name
rap guanine nucleotide exchange factor 2
NCBI Official Synonym Full Names
Rap guanine nucleotide exchange factor (GEF) 2
NCBI Official Symbol
RAPGEF2
NCBI Official Synonym Symbols
RA-GEF; NRAPGEP; PDZGEF1; Rap-GEP; CNrasGEF; PDZ-GEF1; RA-GEF-1; nRap GEP
NCBI Protein Information
rap guanine nucleotide exchange factor 2; cyclic nucleotide ras GEF; ras/Rap1-associating GEF-1; RA(Ras/Rap1A-associating)-GEF; neural RAP guanine nucleotide exchange protein; PDZ domain-containing guanine nucleotide exchange factor 1; PDZ domain containi
UniProt Protein Name
Rap guanine nucleotide exchange factor 2
UniProt Gene Name
RAPGEF2
UniProt Synonym Gene Names
KIAA0313; NRAPGEP; PDZGEF1; CNrasGEF; nRap GEP; PDZ-GEF1
UniProt Entry Name
RPGF2_HUMAN
Similar Products
Product Notes
The PDZ-GEF1 rapgef2 (Catalog #AAA211700) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-PDZ-GEF1 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PDZ-GEF1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the PDZ-GEF1 rapgef2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PDZ-GEF1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.