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FCM/FACS (Flow Cytometry)
(PCM-1 Antibody (Center) flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit anti-Human PCM-1 Polyclonal Antibody | anti-PCM1 antibody
PCM-1 Antibody (Center)
Gene Names
PCM1; PTC4; RET/PCM-1
Reactivity
Human
Applications
Immunofluorescence, Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
PCM-1, Antibody; PCM-1 Antibody (Center); Pericentriolar material 1 protein; PCM-1; hPCM-1; PCM1; anti-PCM1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This PCM-1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 632-661 amino acids from the Central region of human PCM-1.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Positions
632-661
Sequence Length
530
Applicable Applications for anti-PCM1 antibody
IF (Immunofluorescence), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Clone Names
RB18191
Antigen Source
HUMAN
Function
Required for centrosome assembly and function. Essential for the correct localization of several centrosomal proteins including CEP250, CETN3, PCNT and NEK2. Required to anchor microtubules to the centrosome. Involved in the biogenesis of cilia.
Cellular Location
Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome Cytoplasmic granule. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriolar satellite. Cytoplasm, cytoskeleton, cilium basal body. Note=Recruitement to the centrosome requires microtubules and dynein. The majority of the protein dissociates from the centrosome during metaphase and subsequently localizes to the cleavage site in telophase Displaced from centriolar satellites and centrosome in response to cellular stress, such as ultraviolet light (UV) radiation or heat shock, in a process that requires p38 MAP kinase signaling
Tissue Location
Expressed in blood, bone marrow, breast, lymph node, ovary and thyroid.
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(PCM-1 Antibody (Center) flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(PCM-1 Antibody (Center) flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohistochemisry)
(Formalin-fixed and paraffin-embedded human breast carcinoma reacted with PCM-1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
IHC (Immunohistochemisry)
(Formalin-fixed and paraffin-embedded human breast carcinoma reacted with PCM-1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
WB (Western Blot)
(Western blot analysis of PCM-1 antibody (Center) in Ramos cell line lysates (35ug/lane). PCM-1 (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of PCM-1 antibody (Center) in Ramos cell line lysates (35ug/lane). PCM-1 (arrow) was detected using the purified Pab.)
IF (Immunofluorescence)
(Fluorescent image of A549 cell stained with PCM-1 Antibody (Center). A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with PCM-1 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).PCM-1 immunoreactivity is localized to Centrosome significantly.)
IF (Immunofluorescence)
(Fluorescent image of A549 cell stained with PCM-1 Antibody (Center). A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with PCM-1 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).PCM-1 immunoreactivity is localized to Centrosome significantly.)
Related Product Information for anti-PCM1 antibody
PCM-1 is required for centrosome assembly and function. This protein is essential for the correct localization of several centrosomal proteins including CEP250, CETN3, PCNT and NEK2. The protein is required to anchor microtubules to the centrosome.
Product Categories/Family for anti-PCM1 antibody
References
Balczon R., Bao L.J. Cell Biol. 124:783-793(1994)
Corvi R., Berger N.Oncogene 19:4236-4242(2000)
Dammermann A., Merdes A.J. Cell Biol. 159:255-266(2002)
Reiter A., Walz C.Cancer Res. 65:2662-2667(2005)
Murati A., Gelsi-Boyer V.Leukemia 19:1692-1696(2005)
Hames R.S., Crookes R.E.Mol. Biol. Cell 16:1711-1724(2005)
Corvi R., Berger N.Oncogene 19:4236-4242(2000)
Dammermann A., Merdes A.J. Cell Biol. 159:255-266(2002)
Reiter A., Walz C.Cancer Res. 65:2662-2667(2005)
Murati A., Gelsi-Boyer V.Leukemia 19:1692-1696(2005)
Hames R.S., Crookes R.E.Mol. Biol. Cell 16:1711-1724(2005)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
228533
NCBI Official Full Name
pericentriolar material 1 protein
NCBI Official Synonym Full Names
pericentriolar material 1
NCBI Official Symbol
PCM1
NCBI Official Synonym Symbols
PTC4; RET/PCM-1
NCBI Protein Information
pericentriolar material 1 protein
UniProt Protein Name
Pericentriolar material 1 protein
UniProt Gene Name
PCM1
UniProt Synonym Gene Names
PCM-1; hPCM-1
UniProt Entry Name
PCM1_HUMAN
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Product Notes
The PCM1 pcm1 (Catalog #AAA287909) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 632-661. The PCM-1 Antibody (Center) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PCM-1 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the PCM1 pcm1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PCM-1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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