IHC (Immunohiostchemistry)
(Immunochemical staining of human PC in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
Rabbit anti-Human PC/pyruvate carboxylase Polyclonal Antibody | anti-PC antibody
Anti-PC/pyruvate carboxylase Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Immunohistochemistry
Purity
Protein A & Antigen Affinity
Synonyms
PC/pyruvate carboxylase, Antibody; Anti-PC/pyruvate carboxylase Antibody, Rabbit Polyclonal; PC/pyruvate carboxylase Antibody, Rabbit PAb, Antigen Affinity Purified; Anti-PCB Antibody; pyruvate carboxylase; anti-PC antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human PC/pyruvate carboxylase
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-PC antibody
IHC (Immunohistochemistry)
Conjugation
Unconjugated
Immunogen
E Coli-derived Human PC/pyruvate carboxylase fragment
Preparation
Produced in rabbits immunized with E Coli-derived Human PC/pyruvate carboxylase fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2 degree C-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
IHC (Immunohiostchemistry)
(Immunochemical staining of human PC in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohiostchemistry)
(Immunochemical staining of human PC in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohistochemistry)
(Immunochemical staining of human PC in human liver with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohistochemistry)
(Immunochemical staining of human PC in human liver with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
Related Product Information for anti-PC antibody
Pyruvate carboxylase (PC), an anaplerotic enzyme, plays an essential role in various cellular metabolic pathways including gluconeogenesis, de novo fatty acid synthesis, amino acid synthesis, and glucose-induced insulin secretion. Pyruvate carboxylase catalyzes the HCO(3)(-)- and MgATP-dependent carboxylation of pyruvate to form oxaloacetate. This is a very important anaplerotic reaction, replenishing oxaloacetate withdrawn from the tricarboxylic acid cycle for various pivotal biochemical pathways. PC is therefore considered as an enzyme that is crucial for intermediary metabolism, controlling fuel partitioning toward gluconeogenesis or lipogenesis and in insulin secretion. This large enzyme is multifunctional, and each subunit contains two active sites that catalyze two consecutive reactions that lead to the carboxylation of pyruvate into oxaloacetate, and a binding site for acetyl-CoA, an allosteric regulator of the enzyme.
References
Wyatt BN, et al. (2018) A high-throughput screening assay for pyruvate carboxylase. Anal Biochem 550 90-98.Valle M (2017) "Pyruvate carboxylase, structure and function". Subcell Biochem 83 291-322.Lao-On U, et al. (2018) Roles of pyruvate carboxylase in human diseases: From diabetes to cancers and infection. J Mol Med (Berl) 96 (3-4): 237-247.Jitrapakdee S, et al. (2008) Structure, mechanism and regulation of pyruvate carboxylase. Biochem J 413 (3): 369-387.
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Product Notes
The PC (Catalog #AAA258543) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-PC/pyruvate carboxylase Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PC/pyruvate carboxylase can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the PC for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PC/pyruvate carboxylase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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