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ICC (Immunocytochemistry)
(Anti-Paxillin antibody, AAA45436, ICCICC: HELA Cell)
Rabbit Paxillin Polyclonal Antibody | anti-PXN antibody
Anti-Paxillin antibody
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Immunohistochemistry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Synonyms
Paxillin, Antibody; Anti-Paxillin antibody; Paxillin; paxillin; FLJ16691 antibody; FLJ23042 antibody; Paired box protein Pax 1 antibody; PAX 1 antibody; PAX1 antibody; PAXI_HUMAN antibody; Paxillin alpha antibody; Paxillin antibody; PXN antibody; PXN protein antibody; anti-PXN antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Sequence Length
591
Applicable Applications for anti-PXN antibody
ICC (Immunocytochemistry), IHC (Immunohistochemistry), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminal of human Paxillin (456-472aa HEKDGKAYCRKDYFDMF), identical to the related mouse and rat sequences.
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
ICC (Immunocytochemistry)
(Anti-Paxillin antibody, AAA45436, ICCICC: HELA Cell)
ICC (Immunocytochemistry)
(Anti-Paxillin antibody, AAA45436, ICCICC: HELA Cell)
IHC (Immunohistochemisry)
(Anti-Paxillin antibody, AAA45436, IHC(F)IHC(F): Rat Kidney Tissue)
IHC (Immunohistochemisry)
(Anti-Paxillin antibody, AAA45436, IHC(F)IHC(F): Rat Kidney Tissue)
IHC (Immunohiostchemistry)
(Anti-Paxillin antibody, AAA45436, IHC(P)IHC(P): Human Lung Cancer Tissue)
IHC (Immunohiostchemistry)
(Anti-Paxillin antibody, AAA45436, IHC(P)IHC(P): Human Lung Cancer Tissue)
WB (Western Blot)
(Anti-Paxillin antibody, AAA45436, Western blottingLane 1: 293T Cell LysateLane 2: HELA Cell LysateLane 3: MCF-7 Cell LysateLane 4: MM231 Cell LysateLane 5: JUKAT Cell Lysate)
WB (Western Blot)
(Anti-Paxillin antibody, AAA45436, Western blottingLane 1: 293T Cell LysateLane 2: HELA Cell LysateLane 3: MCF-7 Cell LysateLane 4: MM231 Cell LysateLane 5: JUKAT Cell Lysate)
Related Product Information for anti-PXN antibody
Description: Rabbit IgG polyclonal antibody for Paxillin(PXN) detection. Tested with WB, IHC-P, IHC-F, ICC in Human, Mouse, Rat.
Background: PXN(Paxillin) is a signal transduction adaptor protein discovered in 1990 in the laboratory of Keith Burridge. Salgia et al.(1995) mapped the paxillin gene to 12q24 using fluorescence in situ hybridization.The C-terminal region of paxillin contains four LIM domains that target paxillin to focal adhesions, it is presumed through a direct association with the cytoplasmic tail of beta-integrin. The N-terminal region of paxillin is rich in protein-protein interaction sites. The proteins that bind to paxillin are diverse and include protein tyrosine kinases, such as Src and FAK, structural proteins, such as vinculin and actopaxin, and regulators of actin organization, such as COOL/PIX and PKL/GIT. Paxillin is tyrosine-phosphorylated by FAK and Src upon integrin engagement or growth factor stimulation, creating binding sites for the adapter protein Crk.The paxillin protein contains 4 LIM domains, a proline-rich domain containing a consensus SH3-binding site, and 3 potential SH2-binding sites. On Northern blots, paxillin was expressed as a 3.7-kb mRNA in all tissues tested.
Background: PXN(Paxillin) is a signal transduction adaptor protein discovered in 1990 in the laboratory of Keith Burridge. Salgia et al.(1995) mapped the paxillin gene to 12q24 using fluorescence in situ hybridization.The C-terminal region of paxillin contains four LIM domains that target paxillin to focal adhesions, it is presumed through a direct association with the cytoplasmic tail of beta-integrin. The N-terminal region of paxillin is rich in protein-protein interaction sites. The proteins that bind to paxillin are diverse and include protein tyrosine kinases, such as Src and FAK, structural proteins, such as vinculin and actopaxin, and regulators of actin organization, such as COOL/PIX and PKL/GIT. Paxillin is tyrosine-phosphorylated by FAK and Src upon integrin engagement or growth factor stimulation, creating binding sites for the adapter protein Crk.The paxillin protein contains 4 LIM domains, a proline-rich domain containing a consensus SH3-binding site, and 3 potential SH2-binding sites. On Northern blots, paxillin was expressed as a 3.7-kb mRNA in all tissues tested.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
46,604 Da
NCBI Official Full Name
Paxillin
NCBI Official Synonym Full Names
paxillin
NCBI Official Symbol
PXN
NCBI Protein Information
paxillin
UniProt Protein Name
Paxillin
UniProt Gene Name
PXN
UniProt Entry Name
PAXI_HUMAN
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Product Notes
The PXN pxn (Catalog #AAA45436) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Paxillin antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Paxillin can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), IHC (Immunohistochemistry), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the PXN pxn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Paxillin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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