WB (Western Blot)
(Western blot analysis of PAF1 (arrow) using rabbit polyclonal PAF1 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PAF1 gene.)
Rabbit PAF1 Polyclonal Antibody | anti-PAF1 antibody
PAF1 Antibody (C-term)
Gene Names
PAF1; PD2; F23149_1
Reactivity
Human (Predicted Reactivity: Bovine, Mouse, Rat)
Applications
ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
PAF1, Antibody; PAF1 Antibody (C-term); RNA polymerase II-associated factor 1 homolog; hPAF1; Pancreatic differentiation protein 2; PAF1; PD2; anti-PAF1 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Bovine, Mouse, Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This PAF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 439-467 amino acids from the C-terminal region of human PAF1.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.4 (varies by lot)
Sequence Positions
439-467
Sequence Length
485
Applicable Applications for anti-PAF1 antibody
ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(Western blot analysis of PAF1 (arrow) using rabbit polyclonal PAF1 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PAF1 gene.)
WB (Western Blot)
(Western blot analysis of PAF1 (arrow) using rabbit polyclonal PAF1 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PAF1 gene.)
WB (Western Blot)
(PAF1 Antibody (C-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the PAF1 antibody detected the PAF1 protein (arrow).)
WB (Western Blot)
(PAF1 Antibody (C-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the PAF1 antibody detected the PAF1 protein (arrow).)
Related Product Information for anti-PAF1 antibody
PAF1, parafibromin (CDC73; MIM 607393), LEO1 (MIM 610507),
and CTR9 (MIM 609366) form the PAF protein complex that associates
with the RNA polymerase II subunit POLR2A (MIM 180660) and with a
histone methyltransferase complex (Rozenblatt-Rosen et al., 2005
[PubMed 15632063]). The PAF complex also has a role in histone
monoubiquitination (Zhu et al., 2005 [PubMed 16307923]).[supplied
by OMIM].
and CTR9 (MIM 609366) form the PAF protein complex that associates
with the RNA polymerase II subunit POLR2A (MIM 180660) and with a
histone methyltransferase complex (Rozenblatt-Rosen et al., 2005
[PubMed 15632063]). The PAF complex also has a role in histone
monoubiquitination (Zhu et al., 2005 [PubMed 16307923]).[supplied
by OMIM].
References
Muntean, A.G., et al. Cancer Cell 17(6):609-621(2010)
Kim, J., et al. Cell 140(4):491-503(2010)
Moniaux, N., et al. PLoS ONE 4 (9), E7077 (2009) :
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Moniaux, N., et al. Oncogene 25(23):3247-3257(2006)
Kim, J., et al. Cell 140(4):491-503(2010)
Moniaux, N., et al. PLoS ONE 4 (9), E7077 (2009) :
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Moniaux, N., et al. Oncogene 25(23):3247-3257(2006)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
59976
NCBI Official Full Name
RNA polymerase II-associated factor 1 homolog isoform 2
NCBI Official Synonym Full Names
Paf1, RNA polymerase II associated factor, homolog (S. cerevisiae)
NCBI Official Symbol
PAF1
NCBI Official Synonym Symbols
PD2; F23149_1
NCBI Protein Information
RNA polymerase II-associated factor 1 homolog
UniProt Protein Name
RNA polymerase II-associated factor 1 homolog
UniProt Gene Name
PAF1
UniProt Synonym Gene Names
PD2; hPAF1
UniProt Entry Name
PAF1_HUMAN
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Product Notes
The PAF1 paf1 (Catalog #AAA289752) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 439-467. The PAF1 Antibody (C-term) reacts with Human (Predicted Reactivity: Bovine, Mouse, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's PAF1 can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the PAF1 paf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PAF1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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