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WB (Western Blot)
(Western blot analysis of extracts from 293 cells (Lane 2), using p14 ARF antiobdy. The lane on the left is treated with synthesized peptide.)
Rabbit anti-Human p14 ARF Polyclonal Antibody | anti-p14 ARF antibody
p14 ARF Antibody
Gene Names
CDKN2A; ARF; MLM; P14; P16; P19; CMM2; INK4; MTS1; TP16; CDK4I; CDKN2; INK4A; MTS-1; P14ARF; P19ARF; P16INK4; P16INK4A; P16-INK4A
Reactivity
Human
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
p14 ARF, Antibody; p14 ARF Antibody; CDK4I; CMM2; MLM; MTS1; TP16; anti-p14 ARF antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody detects endogenous levels of total p14 ARF protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1.0 mg/ml (varies by lot)
Sequence Length
132
Applicable Applications for anti-p14 ARF antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen Type
Peptide
Immunogen Description
Synthesized peptide derived from human p14 ARF antibody.
Target Name
p14 ARF
Preparation and Storage
Store at -20 degree C
WB (Western Blot)
(Western blot analysis of extracts from 293 cells (Lane 2), using p14 ARF antiobdy. The lane on the left is treated with synthesized peptide.)
WB (Western Blot)
(Western blot analysis of extracts from 293 cells (Lane 2), using p14 ARF antiobdy. The lane on the left is treated with synthesized peptide.)
WB (Western Blot)
(Western blot analysis of extracts from HeLa cells, using p14 ARF antibody.)
WB (Western Blot)
(Western blot analysis of extracts from HeLa cells, using p14 ARF antibody.)
IF (Immunofluorescence)
(Immunofluorescence analysis of HeLa cells, using p14 ARF antibody.)
IF (Immunofluorescence)
(Immunofluorescence analysis of HeLa cells, using p14 ARF antibody.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human placenta tissue using p14 ARF antibody.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human placenta tissue using p14 ARF antibody.)
Related Product Information for anti-p14 ARF antibody
Capable of inducing cell cycle arrest in G1 and G2 phases. Acts as a tumor suppressor. Binds to MDM2 and blocks its nucleocytoplasmic shuttling by sequestering it in the nucleolus. This inhibits the oncogenic action of MDM2 by blocking MDM2-induced degradation of p53 and enhancing p53-dependent transactivation and apoptosis. Also induces G2 arrest and apoptosis in a p53-independent manner by preventing the activation of cyclin B1/CDC2 complexes. Binds to BCL6 and down-regulates BCL6-induced transcriptional repression. Binds to E2F1 and MYC and blocks their transcriptional activator activity but has no effect on MYC transcriptional repression. Binds to TOP1/TOPOI and stimulates its activity. This complex binds to rRNA gene promoters and may play a role in rRNA transcription and/or maturation. Interacts with NPM1/B23 and promotes its polyubiquitination and degradation, thus inhibiting rRNA processing. Interacts with COMMD1 and promotes its 'Lys63'-linked polyubiquitination. Interacts with UBE2I/UBC9 and enhances sumoylation of a number of its binding partners including MDM2 and E2F1. Binds to HUWE1 and represses its ubiquitin ligase activity. May play a role in controlling cell proliferation and apoptosis during mammary gland development. Isoform 6 may be involved in regulation of autophagy and caspase-independent cell death; the short-lived mitochondrial isoform isstabilized by C1QBP. UniProtKB Q64364B¨¦atrice Eymin, Mol. Cell. Biol., Jun 2006; 26: 4339 - 4350. Guillaume Normand, J. Biol. Chem., Feb 2005; 280: 7118 - 7130. Abel S¨¢nchez-Aguilera, Blood, Feb 2002; 99: 1411 - 1418. Manel Esteller, Cancer Res., Apr 2001; 61: 2816 - 2821.
Product Categories/Family for anti-p14 ARF antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
16,533 Da
NCBI Official Full Name
cyclin-dependent kinase inhibitor 2A isoform p14ARF
NCBI Official Synonym Full Names
cyclin-dependent kinase inhibitor 2A
NCBI Official Symbol
CDKN2A
NCBI Official Synonym Symbols
ARF; MLM; P14; P16; P19; CMM2; INK4; MTS1; TP16; CDK4I; CDKN2; INK4A; MTS-1; P14ARF; P19ARF; P16INK4; P16INK4A; P16-INK4A
NCBI Protein Information
cyclin-dependent kinase inhibitor 2A
UniProt Protein Name
Tumor suppressor ARF
UniProt Gene Name
CDKN2A
UniProt Synonym Gene Names
ARF
UniProt Entry Name
ARF_HUMAN
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Product Notes
The p14 ARF cdkn2a (Catalog #AAA306725) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The p14 ARF Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's p14 ARF can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the p14 ARF cdkn2a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "p14 ARF, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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