IHC (Immunohiostchemistry)
(Anti- Optineurin Picoband antibody, AAA46444, IHC(P)IHC(P): Human Placenta Tissue)
anti-Human Optineurin Polyclonal Antibody | anti-OPTN antibody
Anti-Optineurin Antibody
Gene Names
OPTN; NRP; FIP2; HIP7; HYPL; ALS12; GLC1E; TFIIIA-INTP
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Immunogen Affinity Purified
Synonyms
Optineurin, Antibody; Anti-Optineurin Antibody; Optineurin; 14.7K interacting protein; Ag9 C5; ALS12; E3 14.7K interacting protein; E3-14.7K-interacting protein; FIP 2; FIP-2; FIP2; Glaucoma 1 open angle E (adult onset); Glaucoma 1 open angle E; GLC1E; HIP 7; HIP-7; HIP7; Huntingtin interacting protein 7; Huntingtin interacting protein HYPL; Huntingtin interacting protein L; Huntingtin yeast partner L; Huntingtin-interacting protein 7; Huntingtin-interacting protein L; HYPL; Injury inducible protein I 55; NEMO related protein; NEMO-related protein; NRP; Optic neuropathy inducing protein; Optic neuropathy-inducing protein; OPTN; OPTN_HUMAN; TFIIIA IntP; TFIIIA-IntP; Transcription factor IIIA interacting protein; Transcription factor IIIA-interacting protein; Tumor necrosis factor alpha inducible cellular protein containing leucine zipper domains; optineurin; anti-OPTN antibody
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Immunogen Affinity Purified
Form/Format
Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Sequence Length
577
Applicable Applications for anti-OPTN antibody
IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
E Coli-derived human Optineurin recombinant protein (Position: R241-I577). Human Optineurin shares 82% amino acid (aa) sequence identity with both mouse and rat Optineurin.
Ig Type
Rabbit IgG
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
IHC (Immunohiostchemistry)
(Anti- Optineurin Picoband antibody, AAA46444, IHC(P)IHC(P): Human Placenta Tissue)
IHC (Immunohiostchemistry)
(Anti- Optineurin Picoband antibody, AAA46444, IHC(P)IHC(P): Human Placenta Tissue)
WB (Western Blot)
(Anti- Optineurin Picoband antibody, AAA46444, Western blottingAll lanes: Anti Optineurin (AAA46444) at 0.5ug/mlLane 1: HELA Whole Cell Lysate at 40ugLane 2: U87 Whole Cell Lysate at 40ugLane 3: SMMC Whole Cell Lysate at 40ugLane 4: HT1080 Whole Cell Lysate at 40ugPredicted bind size: 66KDObserved bind size: 66KD)
WB (Western Blot)
(Anti- Optineurin Picoband antibody, AAA46444, Western blottingAll lanes: Anti Optineurin (AAA46444) at 0.5ug/mlLane 1: HELA Whole Cell Lysate at 40ugLane 2: U87 Whole Cell Lysate at 40ugLane 3: SMMC Whole Cell Lysate at 40ugLane 4: HT1080 Whole Cell Lysate at 40ugPredicted bind size: 66KDObserved bind size: 66KD)
Related Product Information for anti-OPTN antibody
Description: Rabbit IgG polyclonal antibody for Optineurin(OPTN) detection. Tested with WB, IHC-P in Human.
Background: OPTN is also known as NRP, FIP2 or HYPL. This gene encodes the coiled-coil containing protein optineurin. Optineurin may play a role in normal-tension glaucoma and adult-onset primary open angle glaucoma. Optineurin interacts with adenovirus E3-14.7K protein and may utilize tumor necrosis factor-alpha or Fas-ligand pathways to mediate apoptosis, inflammation or vasoconstriction. Optineurin may also function in cellular morphogenesis and membrane trafficking, vesicle trafficking, and transcription activation through its interactions with the RAB8, huntingtin, and transcription factor IIIA proteins. Alternative splicing results in multiple transcript variants encoding the same protein.
Background: OPTN is also known as NRP, FIP2 or HYPL. This gene encodes the coiled-coil containing protein optineurin. Optineurin may play a role in normal-tension glaucoma and adult-onset primary open angle glaucoma. Optineurin interacts with adenovirus E3-14.7K protein and may utilize tumor necrosis factor-alpha or Fas-ligand pathways to mediate apoptosis, inflammation or vasoconstriction. Optineurin may also function in cellular morphogenesis and membrane trafficking, vesicle trafficking, and transcription activation through its interactions with the RAB8, huntingtin, and transcription factor IIIA proteins. Alternative splicing results in multiple transcript variants encoding the same protein.
References
1. Johnson L, et al. Screening for OPTN mutations in a cohort of British amyotrophic lateral sclerosis patients. Neurobiol Aging, 2012 Dec. 2. Michou L, et al. Genetic association study of UCMA/GRP and OPTN genes (PDB6 locus) with Paget's disease of bone. Bone, 2012 Oct. 3. Weishaupt JH, et al. A novel optineurin truncating mutation and three glaucoma-associated missense variants in patients with familial amyotrophic lateral sclerosis in Germany. Neurobiol Aging, 2013 May.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
59,559 Da
NCBI Official Full Name
optineurin
NCBI Official Synonym Full Names
optineurin
NCBI Official Symbol
OPTN
NCBI Official Synonym Symbols
NRP; FIP2; HIP7; HYPL; ALS12; GLC1E; TFIIIA-INTP
NCBI Protein Information
optineurin
UniProt Protein Name
Optineurin
UniProt Gene Name
OPTN
UniProt Synonym Gene Names
FIP2; GLC1E; HIP7; HYPL; NRP; HIP-7; TFIIIA-IntP
UniProt Entry Name
OPTN_HUMAN
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Product Notes
The OPTN optn (Catalog #AAA46444) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-Optineurin Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Optineurin can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the OPTN optn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Optineurin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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