IHC (Immunohiostchemistry)
(NXF1 Antibody (N-term) (AAA290232)immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NXF1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Rabbit anti-Human NXF1 Polyclonal Antibody | anti-NXF1 antibody
NXF1 Antibody (N-term)
Gene Names
NXF1; TAP; MEX67
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
NXF1, Antibody; NXF1 Antibody (N-term); Nuclear RNA export factor 1; Tip-associated protein; Tip-associating protein; mRNA export factor TAP; NXF1; TAP; anti-NXF1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This NXF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human NXF1.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
1-30
Sequence Length
356
Applicable Applications for anti-NXF1 antibody
WB (Western Blot), IHC (Immunohistochemistry), ELISA
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohiostchemistry)
(NXF1 Antibody (N-term) (AAA290232)immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NXF1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(NXF1 Antibody (N-term) (AAA290232)immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NXF1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(NXF1 Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the NXF1 antibody detected the NXF1 protein (arrow).)
WB (Western Blot)
(NXF1 Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the NXF1 antibody detected the NXF1 protein (arrow).)
Related Product Information for anti-NXF1 antibody
This gene is one member of a family of nuclear RNA export
factor genes. Common domain features of this family are a
noncanonical RNP-type RNA-binding domain (RBD), 4 leucine-rich
repeats (LRRs), a nuclear transport factor 2 (NTF2)-like domain
that allows heterodimerization with NTF2-related export protein-1
(NXT1), and a ubiquitin-associated domain that mediates
interactions with nucleoporins. The LRRs and NTF2-like domains are
required for export activity. Alternative splicing seems to be a
common mechanism in this gene family. The encoded protein of this
gene shuttles between the nucleus and the cytoplasm and binds in
vivo to poly(A)+ RNA. It is the vertebrate homologue of the yeast
protein Mex67p. The encoded protein overcomes the mRNA export block
caused by the presence of saturating amounts of CTE (constitutive
transport element) RNA of type D retroviruses. Alternative splicing
results in multiple transcript variants.
factor genes. Common domain features of this family are a
noncanonical RNP-type RNA-binding domain (RBD), 4 leucine-rich
repeats (LRRs), a nuclear transport factor 2 (NTF2)-like domain
that allows heterodimerization with NTF2-related export protein-1
(NXT1), and a ubiquitin-associated domain that mediates
interactions with nucleoporins. The LRRs and NTF2-like domains are
required for export activity. Alternative splicing seems to be a
common mechanism in this gene family. The encoded protein of this
gene shuttles between the nucleus and the cytoplasm and binds in
vivo to poly(A)+ RNA. It is the vertebrate homologue of the yeast
protein Mex67p. The encoded protein overcomes the mRNA export block
caused by the presence of saturating amounts of CTE (constitutive
transport element) RNA of type D retroviruses. Alternative splicing
results in multiple transcript variants.
References
Read, E.K., et al. J. Gen. Virol. 91 (PT 5), 1290-1301 (2010) :
Corbin-Lickfett, K.A., et al. J. Virol. 84(5):2212-2222(2010)
Hautbergue, G.M., et al. Curr. Biol. 19(22):1918-1924(2009)
Uranishi, H., et al. J. Biol. Chem. 284(38):26106-26116(2009)
Ote, I., et al. PLoS ONE 4 (11), E7882 (2009) :
Corbin-Lickfett, K.A., et al. J. Virol. 84(5):2212-2222(2010)
Hautbergue, G.M., et al. Curr. Biol. 19(22):1918-1924(2009)
Uranishi, H., et al. J. Biol. Chem. 284(38):26106-26116(2009)
Ote, I., et al. PLoS ONE 4 (11), E7882 (2009) :
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
70182
NCBI Official Full Name
nuclear RNA export factor 1 isoform 2
NCBI Official Synonym Full Names
nuclear RNA export factor 1
NCBI Official Symbol
NXF1
NCBI Official Synonym Symbols
TAP; MEX67
NCBI Protein Information
nuclear RNA export factor 1
UniProt Protein Name
Nuclear RNA export factor 1
UniProt Gene Name
NXF1
UniProt Synonym Gene Names
TAP
UniProt Entry Name
NXF1_HUMAN
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Product Notes
The NXF1 nxf1 (Catalog #AAA290232) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1-30. The NXF1 Antibody (N-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NXF1 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry), ELISA. Researchers should empirically determine the suitability of the NXF1 nxf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NXF1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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