IHC (Immunohiostchemistry)
(IHC Analysis: Human brain, cortex tissue stained with nNOS, pAb at 20µg/ml.)
Rabbit anti-Mouse, Rat nNOS Polyclonal Antibody | anti-nNOS antibody
nNOS polyclonal antibody
Gene Names
NOS1; NOS; bNOS; nNOS; IHPS1; N-NOS; NC-NOS
Reactivity
Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunohistochemistry
Purity
Protein A affinity purified.
Synonyms
nNOS, Antibody; nNOS polyclonal antibody; Nitric oxide synthase (neuronal); NOS I; nNOS, pAb; anti-nNOS antibody
Host
Rabbit
Reactivity
Mouse, Rat
Clonality
Polyclonal
Specificity
Recognizes mouse and rat nNOS. Detects a band of ~155kDa by Western blot.
Purity/Purification
Protein A affinity purified.
Form/Format
Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
Sequence Length
1434
Applicable Applications for anti-nNOS antibody
WB (Western Blot), IHC (Immunohistochemistry), IHC (Immunohistochemistry)
Immunogen
Synthetic peptide corresponding to the sequence near the C-terminus of human nNOS.
Preparation and Storage
Long Term: -20 degree C
Avoid freeze/thaw cycles.
Avoid freeze/thaw cycles.
IHC (Immunohiostchemistry)
(IHC Analysis: Human brain, cortex tissue stained with nNOS, pAb at 20µg/ml.)
IHC (Immunohiostchemistry)
(IHC Analysis: Human brain, cortex tissue stained with nNOS, pAb at 20µg/ml.)
WB (Western Blot)
(Western blot analysis: Lane 1: MW marker, Lane 2: Rat Brain, Lane 3: Mouse Brain.)
WB (Western Blot)
(Western blot analysis: Lane 1: MW marker, Lane 2: Rat Brain, Lane 3: Mouse Brain.)
Related Product Information for anti-nNOS antibody
The diffusible free radical gas nitric oxide (NO) affects a variety of physiological functions, and is a key regulator of the cardiovascular, nervous, and immune systems. NO is synthesized in many tissues from L-arginine, oxygen, and NADPH by three known isoforms of a heme-containing flavoprotein termed NO synthase (nNOS/NOS-I, iNOS/NOS-II, and eNOS/NOS-III). nNOS is a constitutively expressed neuronal NOS isoform that exits in its latent form until it is activated by the binding of calmodulin follo wing elevation of intracellular calcium levels. The C-terminus of nNOS contains a conserved serine residue, Ser1417, analogous to Ser1177 of the constitutively expressed endothelial NOS isoform (eNOS). Phosphorylation of Ser1417 is believed to regulate nNOS activation, particularly in glucose-sensing neurons, where inhibition of AMPK pathways by glucose and leptin serve to suppress nNOS activity, whereas activation of AMPK by insulin leads to nNOS activation.
Product Categories/Family for anti-nNOS antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
164,779 Da
NCBI Official Full Name
Nitric oxide synthase, brain
NCBI Official Synonym Full Names
nitric oxide synthase 1
NCBI Official Symbol
NOS1
NCBI Official Synonym Symbols
NOS; bNOS; nNOS; IHPS1; N-NOS; NC-NOS
NCBI Protein Information
nitric oxide synthase, brain
UniProt Protein Name
Nitric oxide synthase, brain
UniProt Gene Name
NOS1
UniProt Synonym Gene Names
N-NOS; nNOS
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Product Notes
The nNOS nos1 (Catalog #AAA77016) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The nNOS polyclonal antibody reacts with Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's nNOS can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the nNOS nos1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "nNOS, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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