Rabbit NF-kB p65 (pS276) Polyclonal Antibody | anti-NF-kB p65 antibody

Rabbit anti NF-kB p65 (pS276) Polyclonal Antibody

Cat. Num. AAA71303

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunoprecipitation, ELISA
• Purity: Purified by site-modified Epitope Affinity Purification.
• Synonyms: NF-kB p65 (pS276), Antibody; Rabbit anti NF-kB p65 (pS276) Polyclonal Antibody; nuclear factor kappa B subunit p65; NFkB; anti-NF-kB p65 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This antibody recognizes NFkB p65 with the phosphorylation site Ser276. It does not cross-react with non-phosphospecific peptide.
• Purity/Purification: Purified by site-modified Epitope Affinity Purification.
• Form/Format: Supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer.

• Applicable Applications for anti-NF-kB p65 antibody: WB (Western Blot), IHC (Immunohistochemistry), IP (Immunoprecipitation), ELISA
• Antigen Preparation: A synthetic peptide corresponding to the epitope containing a phosphorylation site Ser 276 of human NFkB/p65 protein. This sequence is identical within human and mouse.
• Positive Control: Kidney Tissue
• Cellular Location: Cell Membrane
• Preparation and Storage: The antibodies are stable for 12 months from date of receipt when stored at -20°C to -70°C. The antibodies can be stored at 2-8°C for three months without detectable loss of activity. Avoid repeated freezeing-thawing cycles.

IHC (Immunohistochemisry)

(Immunohistochemistry: The whole cell pallet Hela (FFPE) stained with Rabbit anti-NFkBp65(pS276) (Cat# AAA71303) at 1:200 for 10 min @ RT. Staining of formalin-fixed tissue requires boiling tissue section in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.)

WB (Western Blot)

(Western Blot: The whole lysate derived from Hela (20ug/lane) immunoblotted by Rabbit anti – NFkBp65(pS276) (Cat# AAA71303) at 1:500. Observed a major immunoreactive band at molecular weight ~65kDa.)

WB (Western Blot)

Related Product Information for anti-NF-kB p65 antibody

NFkB is a heterodimer that consists of a 50 kDa DNA binding subunit (p50/NFkB1)) and a 65 kDa transactivation subunit (p65/RelA). Both of these subunits exhibit sequence homology to the proto-oncogene c-Rel. The p50 has an isoform called p49/p52, and both proteins are derived from the amino-terminal of precursor protein p105 and p100. The p50/p65 heterodimer remains in the cytosol in an inactive form as a complex with its inhibitor, IkB. Upon stimulation of cells by a wide variety of stimuli such as lipopolysaccharide (LPS), pro-inflammatory cytokines (IL-1 & TNF, etc.), and viral infection, IkB is phosphorylated and degraded by proteosome. The active NFkB heterodimer is translocated into the nucleus and induces gene expression. The inhibition of p53 activity is dependent upon phosphorylation of p65 (RelA) at S536 by the upstream kinase IKK beta.

References

Simon Morton, et al. A reinvestigation of the multisite phosphorylation of the transcription factor c-Jun. EMBO J. 2003 August 1; 22 (15): 3876 -3886.