WB (Western Blot)
(Anti-NEK2 Antibody - Western Blot. Western blot analysis is shown using Affinity Purified anti-NEK2 antibody to detect endogenous protein present in an unstimulated mouse A-20 whole cell lysate (arrowhead). Comparison to a molecular weight marker indicates a band of ~52 kD corresponding to NEK2 protein. The blot was incubated with a 1:500 dilution of the antibody at room temperature followed by detection using standard techniques. Personal communication Steven Pelech, Kinexus Inc.)
Rabbit anti-Human NEK2 Polyclonal Antibody | anti-NEK2 antibody
Rabbit Polyclonal to Human NEK2
Gene Names
NEK2; NLK1; RP67; NEK2A; HsPK21; PPP1R111
Reactivity
Human
Applications
ELISA, Western Blot, Immunofluorescence, Immunohistochemistry
Purity
Immunoaffinity Purified
Synonyms
NEK2, Antibody; Rabbit Polyclonal to Human NEK2; Anti-NEK2 Antibody (aa287-299) IHC-plus; NEK2; NIMA-related kinase 2; NEK2A; HsPK21; NimA-like protein kinase 1; NLK1; HSPK 21; NimA-related protein kinase 2; Human NEK2; anti-NEK2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
aa 287-299 of Human NEK2 protein.
Purity/Purification
Immunoaffinity Purified
Form/Format
0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Concentration
1 mg/ml (varies by lot)
Sequence Length
445
Applicable Applications for anti-NEK2 antibody
ELISA, WB (Western Blot), IF (Immunofluorescence), IHC (Immunohistochemistry)
Target Species
Human
Immunogen Description
Synthetic peptide from human NEK2.
Immunogen Type
Synthetic peptide
Immunogen
NEK2 antibody was raised against synthetic peptide from human NEK2.
Antigen Modification
aa287-299
Preparation and Storage
Store vial at -20 degree C or below prior to opening. Dilute only prior to immediate use. Aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing.
WB (Western Blot)
(Anti-NEK2 Antibody - Western Blot. Western blot analysis is shown using Affinity Purified anti-NEK2 antibody to detect endogenous protein present in an unstimulated mouse A-20 whole cell lysate (arrowhead). Comparison to a molecular weight marker indicates a band of ~52 kD corresponding to NEK2 protein. The blot was incubated with a 1:500 dilution of the antibody at room temperature followed by detection using standard techniques. Personal communication Steven Pelech, Kinexus Inc.)
WB (Western Blot)
(Anti-NEK2 Antibody - Western Blot. Western blot analysis is shown using Affinity Purified anti-NEK2 antibody to detect endogenous protein present in an unstimulated mouse A-20 whole cell lysate (arrowhead). Comparison to a molecular weight marker indicates a band of ~52 kD corresponding to NEK2 protein. The blot was incubated with a 1:500 dilution of the antibody at room temperature followed by detection using standard techniques. Personal communication Steven Pelech, Kinexus Inc.)
IHC (Immunohiostchemistry)
(Anti-NEK2 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.)
IHC (Immunohiostchemistry)
(Anti-NEK2 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.)
IHC (Immunohistochemistry)
(Anti-NEK2 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.)
IHC (Immunohistochemistry)
(Anti-NEK2 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.)
Product Categories/Family for anti-NEK2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
50,909 Da
NCBI Official Full Name
serine/threonine-protein kinase Nek2 isoform 1
NCBI Official Synonym Full Names
NIMA-related kinase 2
NCBI Official Symbol
NEK2
NCBI Official Synonym Symbols
NLK1; RP67; NEK2A; HsPK21; PPP1R111
NCBI Protein Information
serine/threonine-protein kinase Nek2; nimA-like protein kinase 1; nimA-related protein kinase 2; protein phosphatase 1, regulatory subunit 111; NIMA (never in mitosis gene a)-related kinase 2
UniProt Protein Name
Serine/threonine-protein kinase Nek2
UniProt Gene Name
NEK2
UniProt Synonym Gene Names
NEK2A; NLK1; NimA-related protein kinase 2
UniProt Entry Name
NEK2_HUMAN
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Product Notes
The NEK2 nek2 (Catalog #AAA50762) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit Polyclonal to Human NEK2 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NEK2 can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot), IF (Immunofluorescence), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the NEK2 nek2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NEK2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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