Rabbit NAT8L Polyclonal Antibody | anti-NAT8L antibody

NAT8L Antibody (N-Term)

Cat. Num. AAA290623

• Gene Names: NAT8L; CML3; NACED; NAT8-LIKE
• Reactivity: Human; Predicted : Mouse, Rat
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Synonyms: NAT8L, Antibody; NAT8L Antibody (N-Term); Purified Rabbit Polyclonal Antibody (Pab); N-acetylaspartate synthetase; NAA synthetase; 2.3.1.17; Camello-like protein 3; N-acetyltransferase 8-like protein; NAT8L; CML3; anti-NAT8L antibody

• Host: Rabbit
• Reactivity: Human; Predicted : Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This NAT8L antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 59-93 amino acids from human NAT8L.
• Purity/Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Sequence Length: 302

• Applicable Applications for anti-NAT8L antibody: IHC (Immunohistochemistry), ELISA, WB (Western Blot)
• Function: Catalyzes the synthesis of N-acetylaspartate acid (NAA) from L-aspartate and acetyl-CoA (PubMed:19524112, PubMed:19807691, PubMed:20385109). Promotes dopamine uptake by regulating TNF-alpha expression (By similarity). Attenuates methamphetamine-induced inhibition of dopamine uptake (PubMed:20385109).
• Cellular Location: Cytoplasm. Microsome membrane {ECO:0000250|UniProtKB:D3ZVU9}; Single-pass membrane protein. Mitochondrion membrane; Single-pass membrane protein. Endoplasmic reticulum membrane {ECO:0000250|UniProtKB:Q3UGX3}; Single-pass membrane protein. Note=Its enzymatic activity contribution is quantitatively larger in mitochondrial compartment than in extramitochondrial compartment.
• Tissue Location: Expressed in brain
• Preparation and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

IHC (Immunohiostchemistry)

(Immunohistochemical analysis of AP21925A on paraffin-embedded human kidney tissue was performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:250) for 15min at room temperature. Leica Bond Polymer Refine Detection was used as the secondary antibody.)

WB (Western Blot)

(Anti-NAT8L Antibody (N-Term) at 1:1000 dilution + U-87 MG whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 33 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-NAT8L antibody

Plays a role in the regulation of lipogenesis by producing N-acetylaspartate acid (NAA), a brain-specific metabolite. NAA occurs in high concentration in brain and its hydrolysis plays a significant part in the maintenance of intact white matter. Promotes dopamine uptake by regulating TNF-alpha expression. Attenuates methamphetamine-induced inhibition of dopamine uptake.

Product Categories/Family for anti-NAT8L antibody

Metabolism; Signal Transduction

References

Hillier L.W.,et al.Nature 434:724-731(2005). Brandenberger R.,et al.Nat. Biotechnol. 22:707-716(2004). Ota T.,et al.Nat. Genet. 36:40-45(2004). Popsueva A.E.,et al.Dev. Biol. 234:483-496(2001). Arun P.,et al.Neurochem. Int. 55:219-225(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 263192221
• NCBI GeneID: 339983
• NCBI Accession #: NP_848652.2
• NCBI GenBank Nucleotide #: NM_178557.3
• UniProt Accession #: Q8N9F0; Q3UGX3; D3ZVU9
• NCBI Official Full Name: N-acetylaspartate synthetase
• NCBI Official Synonym Full Names: N-acetyltransferase 8-like (GCN5-related, putative)
• NCBI Official Symbol: NAT8L
• NCBI Official Synonym Symbols: CML3; NACED; NAT8-LIKE
• NCBI Protein Information: N-acetylaspartate synthetase
• UniProt Protein Name: N-acetylaspartate synthetase
• UniProt Gene Name: NAT8L
• UniProt Synonym Gene Names: CML3; NAA synthetase
• UniProt Entry Name: NAT8L_HUMAN