WB (Western Blot)
(Detection of human and mouse Myosin-10 by western blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug), HEK293T (T; 50 ug) and mouse NIH 3T3 (M; 50ug) cells. Antibodies: Affinity purified rabbit anti-Myosin-10 antibody AAA211735 (lot 302-043A-1) used for WB at 1 ug/ml. Detection: Chemiluminescence with exposure time of 10 seconds.)
Rabbit anti-Human, Mouse Myosin-10 Polyclonal Antibody | anti-MYH10 antibody
Rabbit anti-Myosin-10 Antibody, Affinity Purified
Reactivity
Human, Mouse
Applications
Western Blot
Purity
Antigen Affinity Purified
Synonyms
Myosin-10, Antibody; Rabbit anti-Myosin-10 Antibody, Affinity Purified; nonmuscle myosin II heavy chain-B; Non-muscle myosin heavy chain IIb; nonmuscle myosin heavy chain IIB; Non-muscle myosin heavy chain B; NMMHC-IIB; NMMHC-B; NMMHCB; myosin, heavy chain 10, non-muscle; myosin, heavy polypeptide 10, non-muscle; NMMHC II-b; myosin heavy chain, nonmuscle type B; Myosin heavy chain, non-muscle IIb; Myosin heavy chain 10; cellular myosin heavy chain, type B; myosin-10; Myosin-10; myosin heavy chain 10; anti-MYH10 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-MYH10 antibody
WB (Western Blot)
Predicted Reactivity
Rat, Rabbit
Immunogen
Between 1926 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human and mouse Myosin-10 by western blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug), HEK293T (T; 50 ug) and mouse NIH 3T3 (M; 50ug) cells. Antibodies: Affinity purified rabbit anti-Myosin-10 antibody AAA211735 (lot 302-043A-1) used for WB at 1 ug/ml. Detection: Chemiluminescence with exposure time of 10 seconds.)
WB (Western Blot)
(Detection of human and mouse Myosin-10 by western blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug), HEK293T (T; 50 ug) and mouse NIH 3T3 (M; 50ug) cells. Antibodies: Affinity purified rabbit anti-Myosin-10 antibody AAA211735 (lot 302-043A-1) used for WB at 1 ug/ml. Detection: Chemiluminescence with exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human Myosin-10 by western blot of immunoprecipitates. Samples: Whole cell lysate from HeLa (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Myosin-10 antibody AAA211735 (lot 302-043A-1) used for IP at 3 ug/mg lysate. Myosin-10 was also immunoprecipitated by rabbit anti-Myosin-10 antibody which recognizes an upstream epitope. For blotting immunoprecipitated Myosin-10, AAA211735 was used at 1.0 ug/ml. Detection: Chemiluminescence with exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human Myosin-10 by western blot of immunoprecipitates. Samples: Whole cell lysate from HeLa (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Myosin-10 antibody AAA211735 (lot 302-043A-1) used for IP at 3 ug/mg lysate. Myosin-10 was also immunoprecipitated by rabbit anti-Myosin-10 antibody which recognizes an upstream epitope. For blotting immunoprecipitated Myosin-10, AAA211735 was used at 1.0 ug/ml. Detection: Chemiluminescence with exposure time of 3 minutes.)
Related Product Information for anti-MYH10 antibody
Myosin II is a hexameric protein that consist of a pair of heavy chain subunits (MHC), a pair of essential light chain subunits (MLC), and a pair of regulatory light chain subunits (RLCs). Non-muscle myosin heavy chain II-B is encoded by the myosin heavy chain 10 gene (MYH10) and non-muscle myosin heavy chain II-A is encoded by the Myosin heavy chain 9 gene (MYH9). Myosin in non-muscle cells is involved in motile cellular processes which include cytokinesis, cell shape, secrection, and capping.
Product Categories/Family for anti-MYH10 antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The MYH10 myh10 (Catalog #AAA211735) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-Myosin-10 Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Myosin-10 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot). Researchers should empirically determine the suitability of the MYH10 myh10 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Myosin-10, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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