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FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of N2A cells with MLCK antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the secondary antibody.)
Rabbit anti-Human, Mouse MLCK Polyclonal Antibody | anti-MLCK antibody
MLCK Antibody
Gene Names
MYLK; KRP; AAT7; MLCK; MLCK1; MYLK1; smMLCK; MLCK108; MLCK210; MSTP083
Reactivity
Human, Mouse
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, Immunocytochemistry
Purity
Peptide affinity purified
Synonyms
MLCK, Antibody; MLCK Antibody; deglutamylated form antibody; DKFZp686I10125 antibody; EC 2.7.11.18 antibody; FLJ12216 antibody; Kinase related protein antibody; Kinase-related protein antibody; KRP antibody; MLCK antibody; MLCK1 antibody; MLCK108 antibody; MLCK210 antibody; MSTP083 antibody; MYLK antibody; MYLK_HUMAN antibody; MYLK1 antibody; Myosin light chain kinase antibody; Myosin light polypeptide kinase antibody; OTTHUMP00000180642 antibody; OTTHUMP00000180643 antibody; smMLCK antibody; smooth muscle antibody; Smooth muscle myosin light chain kinase antibody; Telokin antibody; anti-MLCK antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Peptide affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-MLCK antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ICC (Immunocytochemistry)
Immunogen
Peptide
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of N2A cells with MLCK antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the secondary antibody.)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of N2A cells with MLCK antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry)
(Immunocytochemical staining of SHG-44 cells using anti-MLCK rabbit polyclonal antibody.)
ICC (Immunocytochemistry)
(Immunocytochemical staining of SHG-44 cells using anti-MLCK rabbit polyclonal antibody.)
ICC (Immunocytochemistry)
(Immunocytochemical staining of NIH/3T3 cells using anti-MLCK rabbit polyclonal antibody.)
ICC (Immunocytochemistry)
(Immunocytochemical staining of NIH/3T3 cells using anti-MLCK rabbit polyclonal antibody.)
ICC (Immunocytochemistry)
(Immunocytochemical staining of Hela cells using anti-MLCK rabbit polyclonal antibody.)
ICC (Immunocytochemistry)
(Immunocytochemical staining of Hela cells using anti-MLCK rabbit polyclonal antibody.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin- embedded mouse smooth muscle tissue using anti-MLCK rabbit polyclonal antibody.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin- embedded mouse smooth muscle tissue using anti-MLCK rabbit polyclonal antibody.)
Related Product Information for anti-MLCK antibody
The Ca2+/calmodulin-dependent protein kinases (CaM kinases) are a structurally related subfamily of serine/threonine kinases that includes CaMKI, CaMKII, CaMKIV and myosin light chain kinases (MYLK, also designated MLCK). The MYLK kinases phosphorylate myosin regulatory light chains to catalyze myosin interaction with actin filaments resulting in contractile activity. Non-muscle, smooth muscle and skeletal/cardiac muscle MYLK isoforms exist. The MYLK gene (also designated MYLK1) encodes both smooth muscle and non-muscle isoforms as well as telokin, a small C-terminal isoform expressed only in smooth muscle with the capacity to stabilize unphosphorylated myosin filaments. Multiple transcript variants are described for the MYLK gene. Smooth-muscle and non-muscle MYLK isoforms are expressed in a wide variety of adult and fetal tissues. The skeletal/cardiac muscle isoforms of MYLK are encoded by a separate gene, MYLK2 (also designated skMLCK). MYLK appears to be a target for PAKs (p21-activated kinases). PAK1 interaction with MYLK results in a decrease in MYLK activity and myosin light chain phosphorylation.
Product Categories/Family for anti-MLCK antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
210,715 Da
NCBI Official Full Name
myosin light chain kinase, smooth muscle isoform 1
NCBI Official Synonym Full Names
myosin light chain kinase
NCBI Official Symbol
MYLK
NCBI Official Synonym Symbols
KRP; AAT7; MLCK; MLCK1; MYLK1; smMLCK; MLCK108; MLCK210; MSTP083
NCBI Protein Information
myosin light chain kinase, smooth muscle; telokin; kinase-related protein; myosin, light polypeptide kinase; smooth muscle myosin light chain kinase
UniProt Protein Name
Myosin light chain kinase, smooth muscle
UniProt Gene Name
MYLK
UniProt Synonym Gene Names
MLCK; MLCK1; MYLK1; MLCK; smMLCK; KRP
UniProt Entry Name
MYLK_HUMAN
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Product Notes
The MLCK mylk (Catalog #AAA311963) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MLCK Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's MLCK can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ICC (Immunocytochemistry). Researchers should empirically determine the suitability of the MLCK mylk for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MLCK, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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