FCM/FACS (Flow Cytometry)
(MGAT5 Antibody (C-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit anti-Human MGAT5 Polyclonal Antibody | anti-MGAT5 antibody
MGAT5 Antibody (C-term)
Gene Names
MGAT5; GNT-V; GNT-VA
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
MGAT5, Antibody; MGAT5 Antibody (C-term); Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A; Alpha-mannoside beta-1,6-N-acetylglucosaminyltransferase; GlcNAc-T V; GNT-V; Mannoside acetylglucosaminyltransferase 5; N-acetylglucosaminyl-transferase V; MGAT5; GGNT5; anti-MGAT5 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This MGAT5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 652-680 amino acids from the C-terminal region of human MGAT5.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
652-680
Sequence Length
741
Applicable Applications for anti-MGAT5 antibody
FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(MGAT5 Antibody (C-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(MGAT5 Antibody (C-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)
WB (Western Blot)
(MGAT5 Antibody (C-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the MGAT5 antibody detected the MGAT5 protein (arrow).)
WB (Western Blot)
(MGAT5 Antibody (C-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the MGAT5 antibody detected the MGAT5 protein (arrow).)
Related Product Information for anti-MGAT5 antibody
This gene encodes mannosyl (alpha-1,6-)-glycoprotein
beta-1,6-N-acetyl-glucosaminyltransferase, a glycosyltransferase
involved in the synthesis of protein-bound and lipid-bound
oligosaccharides. Alterations of the oligosaccharides on cell
surface glycoproteins cause significant changes in the adhesive or
migratory behavior of a cell. Increase in the encoded protein's
activity may correlate with the progression of invasive
malignancies.
beta-1,6-N-acetyl-glucosaminyltransferase, a glycosyltransferase
involved in the synthesis of protein-bound and lipid-bound
oligosaccharides. Alterations of the oligosaccharides on cell
surface glycoproteins cause significant changes in the adhesive or
migratory behavior of a cell. Increase in the encoded protein's
activity may correlate with the progression of invasive
malignancies.
Product Categories/Family for anti-MGAT5 antibody
References
Dick, D.M., et al. Am. J. Med. Genet. B Neuropsychiatr. Genet. 153B (6), 1179-1188 (2010) :
Brynedal, B., et al. J. Neuroimmunol. 220 (1-2), 120-124 (2010) :
Benson, V., et al. Int. Immunol. 22(3):167-177(2010)
Wang, C., et al. J. Cell. Biochem. 109(1):113-123(2010)
Ding, H., et al. Stroke 41(1):177-180(2010)
Brynedal, B., et al. J. Neuroimmunol. 220 (1-2), 120-124 (2010) :
Benson, V., et al. Int. Immunol. 22(3):167-177(2010)
Wang, C., et al. J. Cell. Biochem. 109(1):113-123(2010)
Ding, H., et al. Stroke 41(1):177-180(2010)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
84543
NCBI Official Full Name
alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A
NCBI Official Synonym Full Names
mannosyl (alpha-1,6-)-glycoprotein beta-1,6-N-acetyl-glucosaminyltransferase
NCBI Official Symbol
MGAT5
NCBI Official Synonym Symbols
GNT-V; GNT-VA
NCBI Protein Information
alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A
UniProt Protein Name
Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A
UniProt Gene Name
MGAT5
UniProt Synonym Gene Names
GGNT5; GNT-V
UniProt Entry Name
MGT5A_HUMAN
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Product Notes
The MGAT5 mgat5 (Catalog #AAA285313) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 652-680. The MGAT5 Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MGAT5 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the MGAT5 mgat5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MGAT5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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