Chicken MARCKS Polyclonal Antibody | anti-MARCKS antibody

Anti-MARCKS Antibody

Cat. Num. AAA72757

• Gene Names: MARCKS; MACS; 80K-L; PKCSL; PRKCSL
• Reactivity: Human; Predicted: Non-human primate
• Applications: Immunohistochemistry, Immunofluorescence, Western Blot
• Purity: Total IgY Fraction
• Synonyms: MARCKS, Antibody; Anti-MARCKS Antibody; anti-MARCKS antibody

• Host: Chicken
• Reactivity: Human; Predicted: Non-human primate
• Clonality: Polyclonal
• Specificity: Specific for endogenous levels of the ~80 kDa MARCKS protein.
• Purity/Purification: Total IgY Fraction
• Form/Format: Total IgY fraction in PBS + 10 mM NaN3.
• Sequence Length: 332

• Applicable Applications for anti-MARCKS antibody: IHC (Immunohistochemistry), IF (Immunofluorescence), WB (Western Blot)
• Immunogen Species: Human
• Immunogen Information: Recombinant human MARCKS expressed in and purified from E Coli.
• Antigen: Recombinant human MARCKS expressed in and purified from E Coli.
• Quality Control Tests: Western blots performed on each lot.
• Preparation and Storage: Store at -20 degree C in undiluted aliquots; stable for at least 1 year. Avoid freeze/thaw cycles.

IS (Immunostaining)

(Immunostaining of HeLa cells showing specific labeling of the MARCKS protein in red and B-tubulin in green.)

WB (Western Blot)

(Western blot of HeLa lysate showing specific immunolabeling of the ~80 kDa MARCKS protein.)

Related Product Information for anti-MARCKS antibody

Myristoylated Alanine-Rich C Kinase Substrate (MARCKS) is a major substrate for phosphorylation by protein kinase C (PKC) (Ouimet et al.,1990) and is concentrated in synapses of neurons. It appears to function in synaptic vesicle cycling and has been shown to bind both actin and calmodulin in vitro (Hartwig et al. 1992). Deletion of the MARCKS gene in mice results in embryonic brain defects and death (Stumpo et al. 1995). MARCKS is a member of a family of calmodulin binding proteins and phosphorylation of Ser152/156 modulates the binding of MARCKS to calmodulin (Verghese et al., 1994).

References

1. Ouimet CC, Wang JKT, Walaas SI, Albert KA, Greengard P (1990) Localization of the MARCKS (87 kDa) protein, a major specific substrate for protein kinase C, in rat brain. J Neurosci 10:1683-1698. 2. Hartwig JH, Thelen M, Rosen A, Janmey PA, Nairn AC and Aderem A. (1992) MARCKS is an actinfilament crosslinking protein regulated by protein kinase C and calcium-calmodulin. Nature 356: 618-622. 3. Stumpo DJ, Bock CB, Tuttle JS and Blackshear PJ. (1995) MARCKS deficiency in mice leads to abnormal brain development and perinatal death. Proc. Nat. Acad. Sci. 92; 944-948. 4. Verghese GM, Johnson JD, Vasulka C, Haupt DM, Stumpo DJ, Blackshear PJ (1994) Protein kinase C-mediated phosphorylation and calmodulin binding of recombinant myristoylated alanine-rich C kinase substrate (MARCKS) and MARCKS-related protein. J Biol Chem 269:9361-9367.

NCBI and Uniprot Product Information

• NCBI GI #: 153070260
• NCBI GeneID: 4082
• NCBI Accession #: NP_002347.5
• NCBI GenBank Nucleotide #: NM_002356.7
• UniProt Accession #: P29966
• Molecular Weight: ~80 kDa
• NCBI Official Full Name: myristoylated alanine-rich C-kinase substrate
• NCBI Official Synonym Full Names: myristoylated alanine rich protein kinase C substrate
• NCBI Official Symbol: MARCKS
• NCBI Official Synonym Symbols: MACS; 80K-L; PKCSL; PRKCSL
• NCBI Protein Information: myristoylated alanine-rich C-kinase substrate
• UniProt Protein Name: Myristoylated alanine-rich C-kinase substrate
• UniProt Gene Name: MARCKS
• UniProt Synonym Gene Names: MACS; PRKCSL; MARCKS; 80K-L protein; PKCSL
• UniProt Entry Name: MARCS_HUMAN