IF (Immunofluorescence)
(Immunofluorescent analysis of HepG2 cells using AAA232906 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Rabbit anti-Human LIN28B Polyclonal Antibody | anti-LIN28B antibody
LIN28B Antibody
Gene Names
LIN28B; CSDD2
Reactivity
Human
Applications
Immunofluorescence, Immunohistochemistry, ELISA
Purity
>95%, Protein G purified
Synonyms
LIN28B, Antibody; LIN28B Antibody; Protein lin-28 homolog BLin-28B; LIN28B; CSDD2; anti-LIN28B antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
>95%, Protein G purified
Form/Format
Liquid
Sequence Length
250
Applicable Applications for anti-LIN28B antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA
Immunogen
Recombinant Human Protein lin-28 homolog B protein (166-250AA)
Preservative
0.03% Proclin 300
Constituents
50% Glycerol, 0.01M PBS, PH 7.4
Conjugation
Non-conjugated
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-130802
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.
IF (Immunofluorescence)
(Immunofluorescent analysis of HepG2 cells using AAA232906 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
IF (Immunofluorescence)
(Immunofluorescent analysis of HepG2 cells using AAA232906 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human testis tissue using AAA232906 at dilution of 1:100)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human testis tissue using AAA232906 at dilution of 1:100)
Related Product Information for anti-LIN28B antibody
Suppressor of microRNA (miRNA) biogenesis, including that of let-7 and possibly of miR107, miR-143 and miR-200c. Binds primary let-7 transcripts (pri-let-7), including pri-let-7g and pri-let-7a-1, and sequester them in the nucleolus, away from the microprocessor complex, hence preventing their processing into mature miRNA (PubMed: 22118463). Does not act on pri-miR21 (PubMed: 22118463). The repression of let-7 expression is required for normal development and contributes to maintain the pluripotent state of embryonic stem cells by preventing let-7-mediated differentiation. When overexpressed, recruits ZCCHC11/TUT4 uridylyltransferase to pre-let-7 transcripts, leading to their terminal uridylation and degradation (PubMed: 19703396). This activity might not be relevant in vivo, as LIN28B-mediated inhibition of let-7 miRNA maturation appears to be ZCCHC11-independent (PubMed: 22118463). Interaction with target pre-miRNAs occurs via an 5'-GGAG-3' motif in the pre-miRNA terminal loop. Mediates MYC-induced let-7 repression (By similarity). When overexpressed, isoform 1 stimulates growth of the breast adenocarcinoma cell line MCF-7. Isoform 2 has no effect on cell growth.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
19,511 Da
NCBI Official Full Name
protein lin-28 homolog B
NCBI Official Synonym Full Names
lin-28 homolog B
NCBI Official Symbol
LIN28B
NCBI Official Synonym Symbols
CSDD2
NCBI Protein Information
protein lin-28 homolog B
UniProt Protein Name
Protein lin-28 homolog B
UniProt Gene Name
LIN28B
UniProt Synonym Gene Names
CSDD2; Lin-28B
UniProt Entry Name
LN28B_HUMAN
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Product Notes
The LIN28B lin28b (Catalog #AAA232906) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The LIN28B Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's LIN28B can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA. Researchers should empirically determine the suitability of the LIN28B lin28b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LIN28B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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