WB (Western Blot)
(IL12B Antibody (C-term) western blot analysis in CEM,MDA-MB453 cell line lysates (35ug/lane).This demonstrates the IL12B antibody detected the IL12B protein (arrow).)
Rabbit anti-Human IL12B Polyclonal Antibody | anti-IL12B antibody
IL12B Antibody (C-term)
Gene Names
IL12B; CLMF; NKSF; CLMF2; IMD28; IMD29; NKSF2; IL-12B
Reactivity
Human
Applications
Western Blot, Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
IL12B, Antibody; IL12B Antibody (C-term); Interleukin-12 subunit beta; IL-12B; Cytotoxic lymphocyte maturation factor 40 kDa subunit; CLMF p40; IL-12 subunit p40; NK cell stimulatory factor chain 2; NKSF2; IL12B; anti-IL12B antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This IL12B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 264-290 amino acids from the C-terminal region of human IL12B.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
264-290
Sequence Length
328
Applicable Applications for anti-IL12B antibody
WB (Western Blot), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(IL12B Antibody (C-term) western blot analysis in CEM,MDA-MB453 cell line lysates (35ug/lane).This demonstrates the IL12B antibody detected the IL12B protein (arrow).)
WB (Western Blot)
(IL12B Antibody (C-term) western blot analysis in CEM,MDA-MB453 cell line lysates (35ug/lane).This demonstrates the IL12B antibody detected the IL12B protein (arrow).)
FCM/FACS (Flow Cytometry)
(IL12B Antibody (C-term) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(IL12B Antibody (C-term) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohistochemistry)
(IL12B antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the IL12B antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohistochemistry)
(IL12B antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the IL12B antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Related Product Information for anti-IL12B antibody
IL12B is a subunit of interleukin 12, a cytokine
that acts on T and natural killer cells, and has a broad array of
biological activities. Interleukin 12 is a disulfide-linked
heterodimer composed of the 40 kD cytokine receptor like subunit
encoded by this gene, and a 35 kD subunit encoded by IL12A. This
cytokine is expressed by activated macrophages that serve as an
essential inducer of Th1 cells development. This cytokine has been
found to be important for sustaining a sufficient number of
memory/effector Th1 cells to mediate long-term protection to an
intracellular pathogen. Overexpression of this gene was observed in
the central nervous system of patients with multiple sclerosis
(MS), suggesting a role of this cytokine in the pathogenesis of the
disease. The promoter polymorphism of this gene has been reported
to be associated with the severity of atopic and non-atopic asthma
in children.
that acts on T and natural killer cells, and has a broad array of
biological activities. Interleukin 12 is a disulfide-linked
heterodimer composed of the 40 kD cytokine receptor like subunit
encoded by this gene, and a 35 kD subunit encoded by IL12A. This
cytokine is expressed by activated macrophages that serve as an
essential inducer of Th1 cells development. This cytokine has been
found to be important for sustaining a sufficient number of
memory/effector Th1 cells to mediate long-term protection to an
intracellular pathogen. Overexpression of this gene was observed in
the central nervous system of patients with multiple sclerosis
(MS), suggesting a role of this cytokine in the pathogenesis of the
disease. The promoter polymorphism of this gene has been reported
to be associated with the severity of atopic and non-atopic asthma
in children.
Product Categories/Family for anti-IL12B antibody
References
Romero, R., et al. Am. J. Obstet. Gynecol. 203 (4), 361 (2010) :
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Miteva, L.D., et al. Rheumatol. Int. (2010) In press :
de Heens, G.L., et al. J. Clin. Periodontol. 37(2):129-136(2010)
Sobti, R.C., et al. Folia Biol. (Praha) 56(3):110-115(2010)
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Miteva, L.D., et al. Rheumatol. Int. (2010) In press :
de Heens, G.L., et al. J. Clin. Periodontol. 37(2):129-136(2010)
Sobti, R.C., et al. Folia Biol. (Praha) 56(3):110-115(2010)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
37169
NCBI Official Full Name
interleukin-12 subunit beta
NCBI Official Synonym Full Names
interleukin 12B
NCBI Official Symbol
IL12B
NCBI Official Synonym Symbols
CLMF; NKSF; CLMF2; IMD28; IMD29; NKSF2; IL-12B
NCBI Protein Information
interleukin-12 subunit beta
UniProt Protein Name
Interleukin-12 subunit beta
UniProt Gene Name
IL12B
UniProt Synonym Gene Names
NKSF2; IL-12B; CLMF p40; NKSF2
UniProt Entry Name
IL12B_HUMAN
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Product Notes
The IL12B il12b (Catalog #AAA285888) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 264-290. The IL12B Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's IL12B can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA. Researchers should empirically determine the suitability of the IL12B il12b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IL12B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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